scholarly journals Infectious Spondylitis-Associated <i>Staphylococcus aureus</i> with Virulence Gene <i>pvl</i> or <i>tst</i> Causes More Necrosis than Apoptosis in Human Alveolar Basal Epithelial Cell Line A549

2016 ◽  
Vol 06 (07) ◽  
pp. 479-488
Author(s):  
Tsung-Jen Huang ◽  
Chi-Han Lee ◽  
Meng-Huang Wu ◽  
Yen-Yao Li ◽  
Tsung Han Yang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Epithelial Cell ◽  
Cell Line ◽  
Virulence Gene ◽  
Epithelial Cell Line ◽  
Basal Epithelial Cell ◽  
Infectious Spondylitis

Differentiation potential of a basal epithelial cell line established from human bronchial explant

2007 ◽  
Vol 43 (8-9) ◽  
pp. 283-289 ◽  
Author(s):  
Skarphedinn Halldorsson ◽  
Valthor Asgrimsson ◽  
Ivar Axelsson ◽  
Gudmundur Hrafn Gudmundsson ◽  
Margret Steinarsdottir ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Differentiation Potential ◽  
Basal Epithelial Cell

scholarly journals Staphylococcus aureus Escapes More Efficiently from the Phagosome of a Cystic Fibrosis Bronchial Epithelial Cell Line than from Its Normal Counterpart

2006 ◽  
Vol 74 (5) ◽  
pp. 2568-2577 ◽  
Author(s):  
Todd M. Jarry ◽  
Ambrose L. Cheung
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Epithelial Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Microscopic Analysis ◽  
Bronchial Epithelial Cell ◽  
Epithelial Cell Line ◽  
Similar Degree ◽  
Electron Microscopic

ABSTRACTStaphylococcus aureusis frequently the initial bacterium isolated from young cystic fibrosis (CF) patients, and yet its role in CF disease progression has not been determined. Recent data from our lab demonstrates thatS. aureuscan invade and replicate within the CF tracheal epithelial cell line (CFT-1). Here we describe the finding that the fate of internalizedS. aureusin CFT-1 cells differs from its complemented counterpart (LCFSN).S. aureusstrain RN6390 was able to replicate within the mutant CFT-1 cells after invasion but not in the complemented LCFSN cells. At 1 h postinvasion,S. aureuscontaining vesicles within both cell lines acquired vacuolar-ATPase, lysosomal markers LAMP 1 and 2, and the lysomotrophic dye LysoTracker to a similar degree. However, at 4 h postinvasion, the percentage ofS. aureuswithin CFT-1 cells associated with these markers decreased significantly compared to LCFSN, where the association approached 100%. Transmission electron microscopic analysis revealed that the majority of bacteria within CFT-1 cells were free in the cytosol at 4 h after invasion, whereas mostS. aureusbacteria internalized by LCFSN cells remained within vesicles. These results demonstrate a fundamental difference in the fate of liveS. aureusafter invasion of CFT-1 versus LCFSN cell lines and may explain the propensity ofS. aureusto cause chronic lung infection in CF patients.

scholarly journals Staphylococcus aureus RN6390 Replicates and Induces Apoptosis in a Pulmonary Epithelial Cell Line

2000 ◽  
Vol 68 (9) ◽  
pp. 5385-5392 ◽  
Author(s):  
Barbara C. Kahl ◽  
Mark Goulian ◽  
Willem van Wamel ◽  
Mathias Herrmann ◽  
Sanford M. Simon ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Cell Line ◽  
Time Lapse ◽  
Host Cells ◽  
Epithelial Cell Line ◽  
Transmission Electron ◽  
Green Fluorescent ◽  
Respiratory Epithelial

ABSTRACT Staphylococcus aureus frequently colonizes the airways of patients with compromised airway defenses (e.g., cystic fibrosis [CF] patients) for extended periods. Persistent and relapsing infections may be related to live S. aureus bacteria actively residing inside epithelial cells. In this study, we infected a respiratory epithelial cell line, which was derived from a CF patient, with S. aureus RN6390. Internalization of S. aureus was found to be time and dose dependent and could be blocked by cytochalasin D. Transmission electron microscopy revealed that internalized bacteria resided within endocytic vacuoles without any evidence of lysosomal fusion in a 24-h period. The results of internalization experiments and time-lapse fluorescence microscopy of epithelial cells infected with green fluorescent S. aureusindicate that, after an initial lag period of 7 to 9 h, intracellular bacteria began to replicate, with three to five divisions in a 24-h period, leading to apoptosis of infected cells. Induction of apoptosis required bacterial internalization and is associated with intracellular replication. The slow and gradual replication of S. aureus inside epithelial cells hints at the role of host factors or signals in bacterial growth and further suggests possible cross talk between host cells and S. aureus.

An Established Epithelial Cell Line (NPC/HK1) from a Nasopharyngeal Carcinoma - II. A Sem Study

1982 ◽  
Vol 40 ◽  
pp. 224-225
Author(s):  
Li C.L. ◽  
Chew E.C. ◽  
Huang D.P. ◽  
Ho H.C. ◽  
Mak L.S. ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cell ◽  
Surface Morphology ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Cells In Culture ◽  
Sem Study ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been successfully established from a nasopharyngeal carcinoma of the moderately to well differentiated squamous type. The present communication reports on the surface morphology of the NPC/HK1 cells in culture.

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