scholarly journals PCR-based molecular markers linked to the leaf rust resistance gene Lr19 in different bread wheat cultivars

2013 ◽  
Vol 03 (01) ◽  
pp. 153-158 ◽  
Author(s):  
I. M. Huseynova ◽  
F. B. Guliyeva ◽  
S. M. Rustamova ◽  
Jalal A. Aliyev
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene ◽  
Wheat Cultivars

scholarly journals Map-Based Cloning of Leaf Rust Resistance Gene Lr21 From the Large and Polyploid Genome of Bread Wheat

Genetics ◽  
2003 ◽  
Vol 164 (2) ◽  
pp. 655-664 ◽  
Author(s):  
Li Huang ◽  
Steven A Brooks ◽  
Wanlong Li ◽  
John P Fellers ◽  
Harold N Trick ◽  
...  
Keyword(s):  
Amino Acid ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Agronomic Traits ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

Abstract We report the map-based cloning of the leaf rust resistance gene Lr21, previously mapped to a generich region at the distal end of chromosome arm 1DS of bread wheat (Triticum aestivum L.). Molecular cloning of Lr21 was facilitated by diploid/polyploid shuttle mapping strategy. Cloning of Lr21 was confirmed by genetic transformation and by a stably inherited resistance phenotype in transgenic plants. Lr21 spans 4318 bp and encodes a 1080-amino-acid protein containing a conserved nucleotide-binding site (NBS) domain, 13 imperfect leucine-rich repeats (LRRs), and a unique 151-amino-acid sequence missing from known NBS-LRR proteins at the N terminus. Fine-structure genetic analysis at the Lr21 locus detected a noncrossover (recombination without exchange of flanking markers) within a 1415-bp region resulting from either a gene conversion tract of at least 191 bp or a double crossover. The successful map-based cloning approach as demonstrated here now opens the door for cloning of many crop-specific agronomic traits located in the gene-rich regions of bread wheat.

Validation and identification of molecular markers linked to the leaf rust resistance gene Lr28 in wheat

2011 ◽  
Vol 52 (2) ◽  
pp. 171-175 ◽  
Author(s):  
Annapurnalilly Bipinraj ◽  
Balgounda Honrao ◽  
Mohinder Prashar ◽  
Subhash Bhardwaj ◽  
Suryaprakasa Rao ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

Introgression of a leaf rust resistance gene from Aegilops caudata to bread wheat

2012 ◽  
Vol 91 (2) ◽  
pp. 155-161 ◽  
Author(s):  
AMANDEEP KAUR RIAR ◽  
SATINDER KAUR ◽  
H. S. DHALIWAL ◽  
KULDEEP SINGH ◽  
PARVEEN CHHUNEJA
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene ◽  
Aegilops Caudata

Identification and validation of molecular markers linked to the leaf rust resistance gene Lr19 in wheat

2006 ◽  
Vol 113 (6) ◽  
pp. 1027-1036 ◽  
Author(s):  
Sudhir Kumar Gupta ◽  
Ashwini Charpe ◽  
Kumble Vinod Prabhu ◽  
Qazi Mohammad Rizwanul Haque
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

scholarly journals Genetic analysis of leaf rust resistance in three common wheat cultivars MP 3288, HI 784 and HI 1418 under field conditions

2019 ◽  
Vol 79 (01) ◽  
Author(s):  
T. L. Prakasha ◽  
S. Chand ◽  
A. N. Mishra ◽  
K. S. Solanki ◽  
J. B. Singh ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
The Other ◽  
Adult Plant ◽  
Wheat Cultivars ◽  
Seedling Resistance

This study aimed to investigate the genetic basis of leafrust resistance in three bread wheat cultivars viz., MP 3288, HI 1418 and HI 784 which have been maintaining high levels of resistance to leaf rust since their release in 2011, 2000, and 1983, respectively. These cultivars also possess leaf tip necrosis phenotype. These were crossed with a susceptible bread wheat cultivar Lal Bahadur and also among themselves in non-reciprocal manner.The F1 , F2 and F3 populations were raised and the inheritance of leaf rust resistance was studied using prevalent and highly virulent Puccinia triticina pathotype 77-5 (121R63-1) during 2014- 17. These studies showed that the field (adult-plant) resistance of these cultivars is governed by two dominant genes each. Closely linked molecular markers L34DINT9F and L34PLUSR revealed the presence of non-race specific adult-plant leaf rust resistance gene Lr34 in all cultivars of present study. Absence of the other documented race nonspecific APR genes viz., Lr46, Lr67 and Lr68 was indicated in all the three test cultivars based on genotyping with closely linked molecular markers WMC44, CFD71 and csgs, respectively. The other dominant gene appears to be an allstage resistance gene since all the three cultivars displayed high levels of seedling resistance to the test pathotype. Stable resistance of these cultivars could be due to synergistic/additive or complementary effects resulting from the combination of Lr34 and the all-stage resistance gene.

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