scholarly journals Application of Digital PCR in the Analysis of Transgenic Soybean Plants

2016 ◽  
Vol 07 (10) ◽  
pp. 403-417 ◽  
Author(s):  
Jinrong Wan ◽  
Li Song ◽  
Yalei Wu ◽  
Pius Brzoska ◽  
David Keys ◽  
...  
Keyword(s):  
Digital Pcr ◽  
Transgenic Soybean ◽  
Soybean Plants

Photosynthetic response of transgenic soybean plants, containing an Arabidopsis P5CR gene, during heat and drought stress

2004 ◽  
Vol 161 (11) ◽  
pp. 1211-1224 ◽  
Author(s):  
J.A. De Ronde ◽  
W.A. Cress ◽  
G.H.J. Krüger ◽  
R.J. Strasser ◽  
J. Van Staden
Keyword(s):  
Drought Stress ◽  
Photosynthetic Response ◽  
Transgenic Soybean ◽  
Soybean Plants

A universal quantification of transgenic soybean event DAS ‐68416‐4 using duplex digital PCR

2020 ◽  
Vol 101 (2) ◽  
pp. 624-630 ◽  
Author(s):  
Jin Liu ◽  
Zhi‐yong Li ◽  
Jie Dong ◽  
Dong‐wei Gao
Keyword(s):  
Digital Pcr ◽  
Transgenic Soybean ◽  
Universal Quantification

scholarly journals Mapping of transgenic alleles in soybean using a nanopore-based sequencing strategy

2019 ◽  
Vol 70 (15) ◽  
pp. 3825-3833 ◽  
Author(s):  
Shengjun Li ◽  
Shangang Jia ◽  
Lili Hou ◽  
Hanh Nguyen ◽  
Shirley Sato ◽  
...  
Keyword(s):  
High Throughput ◽  
Soybean Genome ◽  
Transgenic Technology ◽  
Transgenic Soybean ◽  
Pooled Dna ◽  
Sequencing Strategy ◽  
Ds Element ◽  
Soybean Plants ◽  
Cost Efficient ◽  
Transgene Insertion

Abstract Transgenic technology was developed to introduce transgenes into various organisms to validate gene function and add genetic variations >40 years ago. However, the identification of the transgene insertion position is still challenging in organisms with complex genomes. Here, we report a nanopore-based method to map the insertion position of a Ds transposable element originating in maize in the soybean genome. In this method, an oligo probe is used to capture the DNA fragments containing the Ds element from pooled DNA samples of transgenic soybean plants. The Ds element-enriched DNAs are then sequenced using the MinION-based platform of Nanopore. This method allowed us to rapidly map the Ds insertion positions in 51 transgenic soybean lines through a single sequencing run. This strategy is high throughput, convenient, reliable, and cost-efficient. The transgenic allele mapping protocol can be easily translated to other eukaryotes with complex genomes.

Gene silencing in transgenic soybean plants transformed via particle bombardment

2003 ◽  
Vol 21 (7) ◽  
pp. 676-683 ◽  
Author(s):  
M. S. Srinivasa Reddy ◽  
Randy D. Dinkins ◽  
Glenn B. Collins
Keyword(s):  
Gene Silencing ◽  
Particle Bombardment ◽  
Transgenic Soybean ◽  
Soybean Plants

Comparative studies focusing on transgenic through cp4EPSPS gene and non-transgenic soybean plants: An analysis of protein species and enzymes

2013 ◽  
Vol 93 ◽  
pp. 107-116 ◽  
Author(s):  
Sandra C.C. Arruda ◽  
Herbert S. Barbosa ◽  
Ricardo A. Azevedo ◽  
Marco A.Z. Arruda
Keyword(s):  
Comparative Studies ◽  
Protein Species ◽  
Transgenic Soybean ◽  
Soybean Plants

scholarly journals CRISPR/Cas9-mediated targeted mutagenesis of GmLCL genes alters plant height and internode length in soybean

2019 ◽  
Author(s):  
Fanjiang Kong ◽  
Qun Cheng ◽  
Lidong Dong ◽  
Tong Su ◽  
Zhuoran Gan ◽  
...  
Keyword(s):  
Plant Height ◽  
New Technology ◽  
Internode Length ◽  
Targeted Mutagenesis ◽  
Myb Transcription Factor ◽  
Soybean Plant ◽  
Transgenic Soybean ◽  
Plant Oil ◽  
Quadruple Mutant ◽  
Soybean Plants

Abstract Background: Soybean ( Glycine max ) is an important economically crops for plant oil and protein in the world. The plant height as a key trait has significant effects on yield of soybean , however, the research on molecular mechanism for soybean plant height is still unclear. Recently, CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (CRISPR-associated) system as a new technology for gene editing, has been rapidly utilized to edit the genomes of crop plants. Results: Here, we designed four gRNAs to mutate four LATE ELONGATED HYPOCOTYL ( LHY ) / CIRCADIAN CLOCK ASSOCIATED1 ( CCA1 )- LIKE ( LCL ) genes in soybean. In order to test whether the gRNAs could perform properly in transgenic soybean plants, we first tested the CRISPR construct in transgenic soybean hairy roots using Agrobacterium rhizogenesis strain K599. Once confirmed, we performed stable soybean transformation and obtained nineteen independent transgenic soybean plants. Subsequently, we obtained one T 1 transgene-free homozygous quadruple mutant of GmLCL by self-crossed. The phenotype of T 2 -generation transgene-free quadruple mutant plants were observed and the results showed that quadruple mutant of GmLCL displayed reduced plant height and shortened internodes. In addition, the relative expression levels of gibberellic acid (GA) metabolic pathway genes in the quadruple mutant of GmLCL were significantly decreased than wild type (WT). It suggests that GmLCLs encoding MYB transcription factor affect plant height through mediating the GA pathway in soybean. We also develop some genetic markers to identify mutant for assisting breeding studies. Conclusions: Our results indicate that CRISPR/Cas9-mediated targeted mutagenesis of four GmLCL genes reduce soybean plant height and shorten internodes. These findings suggest that manipulation of four GmLCL genes may improve yield by altered plant height and internode length in soybean.

scholarly journals GmNFYA13 Improves Salt and Drought Tolerance in Transgenic Soybean Plants

2020 ◽  
Vol 11 ◽  
Author(s):  
Xiao-Jun Ma ◽  
Jin-Dong Fu ◽  
Yi-Miao Tang ◽  
Tai-Fei Yu ◽  
Zhen-Gong Yin ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Transgenic Soybean ◽  
Salt And Drought Tolerance ◽  
Soybean Plants

scholarly journals Overexpression of the GmDREB2 gene increases proline accumulation and tolerance to drought stress in soybean plants

2020 ◽  
pp. 495-503
Author(s):  
Thi Thanh Nhan Pham ◽  
Huu Quan Nguyen ◽  
Thi Ngoc Lan Nguyen ◽  
Xuan Tan Dao ◽  
Danh Thuong Sy ◽  
...  
Keyword(s):  
Glycine Max ◽  
Drought Stress ◽  
Transgenic Plants ◽  
Proline Accumulation ◽  
Stress Conditions ◽  
Transgenic Soybean ◽  
Transcription Level ◽  
Drought Tolerant ◽  
Transgenic Lines ◽  
Soybean Plants

The dehydration responsive element binding (DREB) is a plant protein subfamily expressed when soybean plants face abiotic stresses. These DREB proteins are also considered to activate the transcription of drought-resistant genes. In this study, we present the determined results of relationships between overexpression of Glycine max DREB2 (GmDREB2) with the transcription level of Glycine max pyrroline-5-carboxylate synthetase (GmP5CS) gene, proline accumulation and drought tolerant ability transgenic soybean plants as the basis for selection of transgenic lines with high drought tolerance. GmDREB2 was inserted into a plant transgenic vector and the 35S-GmDREB2-cmyc construct was transferred into the soybean plants by Agrobacterium-mediated transformation. Recombinant GmDREB2 protein with a molecular weight of approximately 20 kDa was expressed in four transgenic soybean lines in the T1 generation. The GmP5CS gene was shown to have significantly (P<0.05) increased expression in the T2 transgenic soybean lines and higher than compared to non-transgenic plants with considering both in well watered condition and stressed conditions were from 1.06 to 1.31-fold and 1.37 to 1.95-fold, respectively. The proline content of four transgenic soybean lines increased 155.81% to 187.42 % after five days in drought-stress conditions and increased from 180.52 % to 233.74 % after nine days under drought-stress conditions (P<0.05). Therefore, the overexpression of GmDREB2 resulted in increasing transcription level of P5CS gene, proline accumulation and drought-stress tolerance of the transgenic soybean plants. The GmDREB2 transformation into soybean plants was confirmed by the results of genetically modified lines in the T2 generation (T2-1, T2-6, T2-7, and T2-8) with higher drought tolerant ability than those of non-transgenic plants.

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