scholarly journals Global Genome Nucleotide Excision Repair Proteins Rhp7p and Rhp41p Are Involved in Abasic Site Repair of <i>Schizosaccharomyces pombe</i>

2015 ◽  
Vol 06 (04) ◽  
pp. 265-274 ◽  
Author(s):  
Eiji Sakurai ◽  
Miyuki Susuki ◽  
Kyoichiro Kanamitsu ◽  
Shinji Kawano ◽  
Shogo Ikeda
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
Abasic Site ◽  
Nucleotide Excision

scholarly journals Contribution of Base Excision Repair, Nucleotide Excision Repair, and DNA Recombination to Alkylation Resistance of the Fission Yeast Schizosaccharomyces pombe

2000 ◽  
Vol 182 (8) ◽  
pp. 2104-2112 ◽  
Author(s):  
Asli Memisoglu ◽  
Leona Samson
Keyword(s):  
Dna Repair ◽  
Schizosaccharomyces Pombe ◽  
Nucleotide Excision Repair ◽  
Base Excision Repair ◽  
Excision Repair ◽  
Dna Recombination ◽  
Nucleotide Excision ◽  
Repair Pathways ◽  
Base Excision

ABSTRACT DNA damage is unavoidable, and organisms across the evolutionary spectrum possess DNA repair pathways that are critical for cell viability and genomic stability. To understand the role of base excision repair (BER) in protecting eukaryotic cells against alkylating agents, we generated Schizosaccharomyces pombe strains mutant for the mag1 3-methyladenine DNA glycosylase gene. We report that S. pombe mag1 mutants have only a slightly increased sensitivity to methylation damage, suggesting that Mag1-initiated BER plays a surprisingly minor role in alkylation resistance in this organism. We go on to show that other DNA repair pathways play a larger role than BER in alkylation resistance. Mutations in genes involved in nucleotide excision repair (rad13) and recombinational repair (rhp51) are much more alkylation sensitive thanmag1 mutants. In addition, S. pombe mutant for the flap endonuclease rad2 gene, whose precise function in DNA repair is unclear, were also more alkylation sensitive thanmag1 mutants. Further, mag1 andrad13 interact synergistically for alkylation resistance, and mag1 and rhp51 display a surprisingly complex genetic interaction. A model for the role of BER in the generation of alkylation-induced DNA strand breaks in S. pombe is discussed.

MITF controls the interface of nucleotide excision repair and transcription through direct regulation of GTF2H1

2015 ◽  
Vol 227 (03) ◽  
Author(s):  
M Seoane ◽  
J Strauss ◽  
AC Puller ◽  
M Noshiravani ◽  
S Feldhaus ◽  
...  
Keyword(s):  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
Nucleotide Excision ◽  
Direct Regulation
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