scholarly journals Hypoglycemia, Hyperglycemia and Astaxanthin: An <i>in Vitro</i> Alzheimer’s Disease Model

2019 ◽  
Vol 08 (03) ◽  
pp. 27-38
Author(s):  
Julie Griffith ◽  
Shauna Northrup ◽  
Emma Cieslik ◽  
Marie Kelly-Worden
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Disease Model

scholarly journals Arrayed CRISPR reveals genetic regulators of tau aggregation, autophagy and mitochondria in Alzheimer’s disease model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lishu Duan ◽  
Mufeng Hu ◽  
Joseph A. Tamm ◽  
Yelena Y. Grinberg ◽  
Fang Shen ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Drug Discovery ◽  
Disease Model ◽  
Mitochondrial Morphology ◽  
Individual Gene ◽  
Human Genes ◽  
Future Drug ◽  
Tau Aggregation

AbstractAlzheimer’s disease (AD) is a common neurodegenerative disease with poor prognosis. New options for drug discovery targets are needed. We developed an imaging based arrayed CRISPR method to interrogate the human genome for modulation of in vitro correlates of AD features, and used this to assess 1525 human genes related to tau aggregation, autophagy and mitochondria. This work revealed (I) a network of tau aggregation modulators including the NF-κB pathway and inflammatory signaling, (II) a correlation between mitochondrial morphology, respiratory function and transcriptomics, (III) machine learning predicted novel roles of genes and pathways in autophagic processes and (IV) individual gene function inferences and interactions among biological processes via multi-feature clustering. These studies provide a platform to interrogate underexplored aspects of AD biology and offer several specific hypotheses for future drug discovery efforts.

Angiotensin II triggers autophagy and apoptosis in PC12 cell line: An in vitro Alzheimer’s disease model

2019 ◽  
Vol 1718 ◽  
pp. 46-52 ◽  
Author(s):  
Minjie Tian ◽  
Xingjian Lin ◽  
Liang Wu ◽  
Jie Lu ◽  
Yingdong Zhang ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Angiotensin Ii ◽  
Cell Line ◽  
Pc12 Cell ◽  
Disease Model ◽  
Pc12 Cell Line

scholarly journals A General Approach to Convert Hemicyanine Dyes into Highly Optimized Photoacoustic Scaffolds for Analyte Sensing

2021 ◽  
Author(s):  
Sarah Garder ◽  
Catharine Brady ◽  
Cameron Keeton ◽  
Anuj K Yadav ◽  
Sharath C Mallojjala ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Photoacoustic Imaging ◽  
Disease Model ◽  
Photoacoustic Signal ◽  
Model Of Alzheimer’S Disease ◽  
5Xfad Mice ◽  
The Impact

<p>In the context of deep-tissue disease biomarker detection and analyte sensing of biologically relevant species, the impact of photoacoustic imaging has been profound. However, most photoacoustic imaging agents to date are based on the repurposing of existing fluorescent dye platforms that exhibit non-optimal properties for photoacoustic applications (e.g., high fluorescence quantum yield). Herein, we introduce two effective modifications to the hemicyanine dye to afford PA-HD, a new dye scaffold optimized for photoacoustic probe development. We observed a significant increase in the photoacoustic output, representing an increase in sensitivity of 4.8-fold and a red-shift of the λ<sub>abs</sub> from 690 nm to 745 nm to enable ratiometric imaging. Moreover, to demonstrate the generalizability and utility of our remodeling efforts, we developed three probes using common analyte-responsive triggers for beta-galactosidase activity (PA-HD-Gal), nitroreductase activity (PA-HD-NTR), and hydrogen peroxide (PA-HD-H<sub>2</sub>O<sub>2</sub>). The performance of each probe (responsiveness, selectivity) was evaluated <i>in vitro</i> and <i>in cellulo</i>. To showcase the enhance properties afforded by PA-HD for <i>in vivo</i> photoacoustic imaging, we employed an Alzheimer’s disease model to detect H<sub>2</sub>O<sub>2</sub>. In particular, the photoacoustic signal at 735 nm in the brains of 5xFAD mice (a murine model of Alzheimer’s disease) increased by 1.72 ± 0.20-fold relative to background indicating the presence of oxidative stress, whereas the change in wildtype mice was negligible (1.02 ± 0.14). These results were confirmed via ratiometric calibration which was not possible using the parent HD platform.</p>

scholarly journals Study of 3-(4’-Nitrobenzamido)coumarin using an Alzheimer’s Disease Model

2021 ◽  
Author(s):  
Fernanda Rodríguez-Enríquez ◽  
Dolores Viña ◽  
Eugenio Uriarte ◽  
José Ángel Fontenla ◽  
Maria João Matos
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Object Recognition ◽  
Disease Model ◽  
Pharmacokinetic Profile ◽  
Ache Inhibitor ◽  
Mice Model ◽  
Object Recognition Test

Abstract 3-(4’-Nitrobenzamido)coumarin (MJM255), a potent in vitro acetylcholinesterase (AChE) inhibitor, was selected as an in vivo candidate for the discovery of new therapeutic solutions for Alzheimer’s disease. Computational (in silico) studies showing the theoretical physicochemical properties indicate desirable a pharmacokinetic profile for this molecule to cross the blood-brain barrier (BBB). An in vivo study, using the object recognition test (ORT) mice model, was carried out. This compound exhibited a similar effect as eserine, a well-known AChE inhibitor able to cross the BBB.

Neuroprotective effects of PrxI over-expression in an in vitro human Alzheimer's disease model

2013 ◽  
Vol 114 (3) ◽  
pp. 708-715 ◽  
Author(s):  
Annamaria Cimini ◽  
Roberta Gentile ◽  
Francesco Angelucci ◽  
Elisabetta Benedetti ◽  
Giuseppina Pitari ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Disease Model ◽  
Neuroprotective Effects ◽  
Over Expression
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