scholarly journals In Vitro Maturation of Oocytes with Consecutive Clinical Pregnancy after Accidental Premature Ovulation Induction-A Rescue Strategy in ART

2021 ◽  
Vol 17 (1) ◽  
pp. 106-110
Author(s):  
S Findeklee
Keyword(s):  
Ovulation Induction ◽  
In Vitro Maturation ◽  
Clinical Pregnancy ◽  
Premature Ovulation

316 EVALUATION OF A HORMONAL PROTOCOL FOR LAPAROSCOPIC OVUM PICK-UP IN PREPUBERTAL EWES

2013 ◽  
Vol 25 (1) ◽  
pp. 305
Author(s):  
P. P. M. Teixeira ◽  
L. C. Padilha ◽  
A. S. L. da Silva ◽  
F. F. P. C. Barros ◽  
L. N. Coutinho ◽  
...  
Keyword(s):  
Ovarian Stimulation ◽  
Ovulation Induction ◽  
High Performance ◽  
In Vitro Maturation ◽  
Prostaglandin F2α ◽  
Hormonal Treatment ◽  
Ovarian Response ◽  
The Other ◽  
Genetic Value

In vitro embryo production is a cutting-edge technology in constant evolution and has been used routinely. With the objective of improving genetic value, recent studies have been focused on the production of high-performance descendants from early prepubertal animals. The aim of this study was to compare hormonal protocols of ovarian stimulation for laparoscopic ovum pick-up in prepubertal sheep, to determine ovarian response and number of oocytes. For this study, 36 Santa Ines sheep, aged between 4 and 8 weeks, were submitted to a progestogen-based, short-term ovulation induction protocol (without use of prostaglandin F2α), and following 36 h, ovarian stimulation (300 IU of eCG) associated with different regimens of administration of FSH, according to experimental group. The ewes were divided into 1 of 6 groups: 2 groups received 80 mg of FSH by either single administration in the first group (G80U) or multiple constant administrations at 12-h intervals in the second group (G80M); animals belonging to 2 other groups received 160 mg in the same fashion, in a single-dose regimen (group G160U) or in multiple administrations (group G160M). The other 2 groups constituted the control groups: one received no hormonal treatment for ovulation induction and ovarian stimulation (GCN); the other received the ovulation induction protocol, but no ovulation stimulation was carried out (GCI). The animals were submitted to laparoscopic ovum pick-up 48 h following the beginning of the ovarian stimulation. The number of follicles viewed (FV), aspirated (FA), and recovered (OR) laparoscopically was recorded. Data were assessed using the one-way ANOVA test, and the comparison among groups was carried out using the Tukey test. The mean of follicles visualised, aspirated, and oocytes recovered are shown in Table 1. Group G160U had a better result regarding OR compared with the other groups. Moreover, the current study highlights that laparoscopic ovum pick-up for in vitro maturation using prepubertal ewes is viable for commercial purposes. More studies are required in order to improve the quality of oocytes and successful in vitro maturation rates. Table 1.Results of visualised follicles (VF), aspirated follicles (AF), and oocytes retrieved (OR)

Clinical-pregnancy outcome after vitrification of blastocysts produced from in vitro maturation cycles

2007 ◽  
Vol 88 (5) ◽  
pp. 1449-1451 ◽  
Author(s):  
Seok-Yoon Lee ◽  
Weon-Young Son ◽  
San-Hyun Yoon ◽  
Jin-Ho Lim
Keyword(s):  
Pregnancy Outcome ◽  
In Vitro Maturation ◽  
Clinical Pregnancy

scholarly journals Enhancing the scope of in vitro maturation for fertility preservation: transvaginal retrieval of immature oocytes during endoscopic gynaecological procedures

2020 ◽  
Vol 35 (4) ◽  
pp. 837-846 ◽  
Author(s):  
Xue-Ling Song ◽  
Cui-Ling Lu ◽  
Xiao-Ying Zheng ◽  
Victoria Nisenblat ◽  
Xiu-Mei Zhen ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Fertility Preservation ◽  
In Vitro Maturation ◽  
Ovarian Function ◽  
Oocyte Retrieval ◽  
Clinical Pregnancy ◽  
Clinical Pregnancy Rate ◽  
Operative Complications ◽  
Transvaginal Oocyte Retrieval

Abstract STUDY QUESTION Could in vitro maturation (IVM) following transvaginal oocyte retrieval during gynaecological surgery (IVM-surgery) be an effective and safe strategy for fertility preservation? SUMMARY ANSWER IVM-surgery on unstimulated ovaries is a novel option that can be considered for fertility preservation for women requiring gynaecological surgery, but more research is needed to identify appropriate patients who may benefit and to determine the cost-effectiveness of such an approach. WHAT IS KNOWN ALREADY IVM followed by oocyte/embryo cryopreservation has been useful as a safe reproductive strategy for some infertile women. STUDY DESIGN, SIZE, DURATION This prospective cohort study comprised 158 consecutive women with polycystic ovary syndrome (PCOS) who underwent laparoscopy or hysteroscopy for other reasons and had concomitant transvaginal oocyte retrieval followed by IVM between 2014 and 2016. PARTICIPANTS/MATERIALS, SETTING, METHODS A total of 158 women with anovulatory PCOS who underwent IVM-surgery in our infertility centre were recruited for this study. Matured IVM oocytes obtained from these women were either freshly fertilized and subsequently frozen at the blastocyst stage (fresh oocyte group, n = 46) or the oocytes were frozen (frozen oocyte group, n = 112) for fertility preservation followed by later thawing for insemination and cleavage embryo transfer (ET) (n = 33). The following outcomes were then evaluated: embryological data, clinical pregnancy rate, live birth rate (LBR), neonatal outcomes, post-operative complications and post-operative ovarian function. MAIN RESULTS AND THE ROLE OF CHANCE Among all the women who underwent IVM-surgery, the clinical pregnancy rate and LBR per initiated IVM cycle were 9.5% (15/158) and 6.9% (11/158), respectively. Women (40.6%, 20/33) who underwent the procedure with frozen-thawed oocytes (oocyte survival rate, 83.0%) obtained a high quality of cleaved embryos. In the fresh oocyte group, the clinical pregnancy rate and LBR per ET cycle were 69.2 and 53.8%, respectively. In the frozen oocyte group, the clinical pregnancy rate and LBR per ET cycle were 28.6 and 19.1%, respectively. No adverse neonatal outcomes were recorded. IVM-surgery was not associated with post-operative complications, a longer hospital stay, or impaired ovarian function. LIMITATIONS, REASONS FOR CAUTION Because of the small sample size and the low utilization rate and cost-effectiveness per retrieval, the present findings should be interpreted with caution, and further studies are needed for the long-term follow-up of live births. WIDER IMPLICATIONS OF THE FINDINGS This strategy can also help patients with normal ovulation to obtain available oocytes and embryos for cryopreservation and subsequent use. STUDY FUNDING/COMPETING INTEREST(S) This research was supported by the Joint Research Fund for Overseas Natural Science of China (No. 31429004), the National Key Research and Development Program of China (No. 2017YFC1002000, 2017YFC1001504, 2016YFC1000302), the Ministry of Science and Technology of China Grants (No. 2014CB943203), the Chinese Society of Reproductive Medicine Fund (No. 16020400656) and the National Natural Science Foundation of China (No. 81300456). All the authors have nothing to disclose in terms of conflicts of interest. TRIAL REGISTRATION NUMBER chictr-ONC-17011861

scholarly journals Live births after oocyte in vitro maturation with a prematuration step in women with polycystic ovary syndrome

2020 ◽  
Vol 37 (2) ◽  
pp. 347-357 ◽  
Author(s):  
Lan N. Vuong ◽  
Anh H. Le ◽  
Vu N. A. Ho ◽  
Toan D. Pham ◽  
Flor Sanchez ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
In Vitro Maturation ◽  
Polycystic Ovary ◽  
Live Birth Rate ◽  
Pregnancy Rates ◽  
Clinical Pregnancy ◽  
Developmental Competence ◽  
Vitro Fertilization ◽  
Live Births

Abstract Purpose Standard oocyte in vitro maturation (IVM) usually results in lower pregnancy rates than in vitro fertilization (IVF). IVM preceded by a prematuration step improves the acquisition of oocyte developmental competence and can enhance embryo quality (EQ). This study evaluated the effectiveness of a biphasic culture system incorporating prematuration and IVM steps (CAPA-IVM) versus standard IVM in women with polycystic ovarian morphology (PCOM). Methods Eighty women (age < 38 years, ≥ 25 follicles of 2–9 mm in both ovaries, no major uterine abnormalities) were randomized to undergo CAPA-IVM (n = 40) or standard IVM (n = 40). CAPA-IVM uses two steps: a 24-h prematuration step with C-type natriuretic peptide-supplemented medium, then 30 h of culture in IVM media supplemented with follicle-stimulating hormone and amphiregulin. Standard IVM was performed using routine protocols. Results A significantly higher proportion of oocytes reached metaphase II at 30 h after CAPA-IVM versus standard IVM (63.6 vs 49.0; p < 0.001) and the number of good quality embryos per cumulus-oocyte complex tended to be higher (18.9 vs 12.7; p = 0.11). Clinical pregnancy rate per embryo transfer was 63.2% in the CAPA-IVM versus 38.5% in the standard IVM group (p = 0.04). Live birth rate per embryo transfer was not statistically different between the CAPA-IVM and standard IVM groups (50.0 vs 33.3% [p = 0.17]). No malformations were reported and birth weight was similar in the two treatment groups. Conclusions Use of the CAPA-IVM system significantly improved maturation and clinical pregnancy rates versus standard IVM in patients with PCOM. Furthermore, live births after CAPA-IVM are reported for the first time.

Effect of isoflavone compounds on the in vitro maturation of sheep oocytes

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
AA Amir ◽  
GB Martin ◽  
JM Kelly ◽  
DO Kleemann ◽  
Z Durmic ◽  
...  
Keyword(s):  
In Vitro Maturation
Sign in / Sign up

Export Citation Format

Share Document