scholarly journals The epidemiology of amoebiasis in Thi-Qar province, Iraq (2015-2020): Differentiation of Entamoeba histolytica/Entamoeba dispar using nested PCR and real-time PCR

2021 ◽  
pp. e2021034
Author(s):  
Mohammed Hassan Flaih ◽  
Ruaa M. Khazaal ◽  
Manar K. Kadhim ◽  
Khwam R. Hussien ◽  
Falah A. B. Alhamadani
Keyword(s):  
Real Time ◽  
Entamoeba Histolytica ◽  
Real Time Pcr ◽  
Nested Pcr ◽  
Entamoeba Dispar

Evaluation of a New Single-Tube Multiprobe Real-Time PCR for Diagnosis of Entamoeba histolytica and Entamoeba dispar

2010 ◽  
Vol 96 (4) ◽  
pp. 793-797 ◽  
Author(s):  
Shih-Yu Liang ◽  
Kan-Tai Hsia ◽  
Yun-Hsien Chan ◽  
Chia-Kwung Fan ◽  
Donald Dah-Shyong Jiang ◽  
...  
Keyword(s):  
Real Time ◽  
Entamoeba Histolytica ◽  
Real Time Pcr ◽  
Entamoeba Dispar ◽  
Single Tube

scholarly journals Evaluation of factors affecting real-time PCR performance for diagnosis of Entamoeba histolytica and Entamoeba dispar in clinical stool samples

2015 ◽  
Vol 64 (9) ◽  
pp. 1053-1062 ◽  
Author(s):  
Joakim Forsell ◽  
Satu Koskiniemi ◽  
Ida Hedberg ◽  
Helén Edebro ◽  
Birgitta Evengård ◽  
...  
Keyword(s):  
Real Time ◽  
Entamoeba Histolytica ◽  
Real Time Pcr ◽  
Entamoeba Dispar ◽  
Factors Affecting ◽  
Stool Samples

scholarly journals Real-Time PCR for Detection and Differentiation of Entamoeba histolytica and Entamoeba dispar in Fecal Samples

2002 ◽  
Vol 40 (12) ◽  
pp. 4413-4417 ◽  
Author(s):  
J. Blessmann ◽  
H. Buss ◽  
P. A. T. Nu ◽  
B. T. Dinh ◽  
Q. T. V. Ngo ◽  
...  
Keyword(s):  
Real Time ◽  
Entamoeba Histolytica ◽  
Real Time Pcr ◽  
Entamoeba Dispar ◽  
Fecal Samples

Identifying the etiologic role of Parvovirus B19 in non-immune hydrops fetalis by histopathology, immunohistochemistry and nucleic acid testing: a retrospective study

Open Medicine ◽  
2007 ◽  
Vol 2 (3) ◽  
pp. 271-279 ◽  
Author(s):  
Koray Ergunay ◽  
Gulcin Altinok ◽  
Bora Gurel ◽  
Ahmet Pinar ◽  
Arzu Sungur ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
San Francisco ◽  
Real Time Pcr ◽  
Nested Pcr ◽  
Parvovirus B19 ◽  
Etiologic Agent ◽  
Hydrops Fetalis ◽  
Nucleic Acid Detection

AbstractIntrauterine Parvovirus B19 infections may cause fetal anemia, non-immune hydrops fetalis or abortion. This study focuses on the pathogenic role of Parvovirus B19 in non-immune hydrops fetalis at Hacettepe University, a major reference hospital in Turkey. Twenty-two cases of non-immune hydrops fetalis were retrospectively selected out of a total of 431 hydrops fetalis specimens from the Department of Pathology archieves. Paraffine embedded tissue sections from placental and liver tissues from each case were evaluated by histopathology, immunohistochemistry, nested PCR and commercial quantitative Real-time PCR. Viral DNA was detected in placental tissues by Real-time PCR in 2 cases (2/22, 9.1%) where histopathology also revealed changes suggestive of Parvovirus B19 infection. No significant histopathologic changes were observed for the remaining sections. Nested PCR that targets the VP1 region of the viral genome and immunohistochemistry for viral capsid antigens were negative for all cases. As a result, Parvovirus B19 is identified as the etiologic agent for the development of non-immune hydrops fetalis for 9.1% of the cases in Hacettepe University, Turkey. Real-time PCR is observed to be an effective diagnostic tool for nucleic acid detection from paraffine embedded tissues. Part of this study was presented as a poster at XIIIth International Congress of Virology, San Francisco, USA (Abstract V-572).

Detection and Quantification of Human Adenoviruses in Surface Waters by Nested PCR, TaqMan Real-Time PCR and Cell Culture Assays

2007 ◽  
Vol 191 (1-4) ◽  
pp. 83-93 ◽  
Author(s):  
M. Muscillo ◽  
M. Pourshaban ◽  
M. Iaconelli ◽  
S. Fontana ◽  
A. Di Grazia ◽  
...  
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
Surface Waters ◽  
Real Time Pcr ◽  
Nested Pcr ◽  
Human Adenoviruses ◽  
Detection And Quantification

scholarly journals Efficient nested-PCR-based method development for detection and genotype identification of Acanthamoeba from a small volume of aquatic environmental sample

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tsui-Kang Hsu ◽  
Jung-Sheng Chen ◽  
Hsin-Chi Tsai ◽  
Chi-Wei Tao ◽  
Yu-Yin Yang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Nested Pcr ◽  
In Silico ◽  
Environmental Samples ◽  
Method Development ◽  
Small Volume ◽  
Detection Efficiency ◽  
Genotyping Method ◽  
Pcr Method

AbstractAcanthamoeba spp. are opportunistic human pathogens that cause granulomatous amoebic encephalitis and keratitis, and their accurate detection and enumeration in environmental samples is a challenge. In addition, information regarding the genotyping of Acanthamoeba spp. using various PCR methods is equally critical. Therefore, considering the diverse niches of habitats, it is necessary to develop an even more efficient genotyping method for Acanthamoeba spp. detection. This study improved the sensitivity of detection to avoid underestimation of Acanthamoeba spp. occurrence in aquatic environmental samples, and to accurately define the pathogenic risk by developing an efficient PCR method. In this study, a new nested genotyping method was established and compared with various PCR-based methods using in silico, lab, and empirical tests. The in silico test showed that many PCR-based methods could not successfully align specific genotypes of Acanthamoeba, except for the newly designed nested PCR and real-time PCR method. Furthermore, 52 water samples from rivers, reservoirs, and a river basin in Taiwan were analysed by six different PCR methods and compared for genotyping and detection efficiency of Acanthamoeba. The newly developed nested-PCR-based method of genotyping was found to be significantly sensitive as it could effectively detect the occurrence of Acanthamoeba spp., which was underestimated by the JDP-PCR method. Additionally, the present results are consistent with previous studies indicating that the high prevalence of Acanthamoeba in the aquatic environment of Taiwan is attributed to the commonly found T4 genotype. Ultimately, we report the development of a small volume procedure, which is a combination of recent genotyping PCR and conventional real-time PCR for enumeration of aquatic Acanthamoeba and acquirement of biologically meaningful genotyping information. We anticipate that the newly developed detection method will contribute to the precise estimation, evaluation, and reduction of the contamination risk of pathogenic Acanthamoeba spp., which is regularly found in the water resources utilised for domestic purposes.

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