Structural Model of TaNAM-B1 Transcription Factor from Wheat (Triticum aestivum) Insight into the Nutritional Grain Quality

2017 ◽  
Vol 10 (8) ◽  
Author(s):  
Murtaza Malik ◽  
Saurabh Pandey ◽  
Kaushlendra Tripathi ◽  
Rashmi Jain ◽  
Tanushri Kaul
Keyword(s):  
Triticum Aestivum ◽  
Transcription Factor ◽  
Grain Quality ◽  
Structural Model ◽  
Insight Into

Effect of algae Ecklonia maxima extract (Kelpak SL) on yields of common wheat, durum wheat and spelt wheat

2019 ◽  
Vol 74 (1) ◽  
pp. 5-14
Author(s):  
GRZEGORZ SZUMIŁO ◽  
LESZEK RACHOŃ ◽  
BARBARA KROCHMAL-MARCZAK
Keyword(s):  
Triticum Aestivum ◽  
Durum Wheat ◽  
Common Wheat ◽  
Yield Components ◽  
Grain Quality ◽  
Foliar Application ◽  
Control Treatment ◽  
Favourable Effect ◽  
Ecklonia Maxima ◽  
Spelt Wheat

The 3-year experiment was concerned with the response of spring forms of common wheat (Triticum aestivum L. subsp. aestivum), durum wheat (Triticum durum Desf.) and spelt wheat (Triticum aestivum subsp. spelta L. em. Thell.) to the foliar application of a plant growth stimulant (extract from marine algae Ecklonia maxima), with the commercial name of Kelpak SL (GS), as compared to control treatment (C). The following parameters were analysed: yield of grain, yield components (number of ears, weight of 1000 kernels, number and weight of kernels per ear) and physical indicators of grain quality (test weight, uniformity and vitreosity of grain). The study showed that the level of yielding and the yield components were related primarily with the wheat genotype, but they depended also on the agro-climatic conditions and on the algae extract and control experimental treatments. The application of algae extract, compared to the control, caused a significant increase in the yields of the spring wheat species under study, on average by 7.0%. Canopy spraying with algae extract had a favourable effect on the number of ears, on he number and weight of kernels per ear, but it had no effect on the weight of 1000 kernels. The grain quality of durum wheat, spelt wheat and common wheat was affected more strongly by the weather conditions in the successive years of the study and by the genotype than by the foliar application of algae extract. The spelt genotypes were characterised by lower yields and lower grain quality than common wheat and the durum wheat genotypes.

scholarly journals The apple C2H2-type zinc finger transcription factor MdZAT10 positively regulates JA-induced leaf senescence by interacting with MdBT2

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Kuo Yang ◽  
Jian-Ping An ◽  
Chong-Yang Li ◽  
Xue-Na Shen ◽  
Ya-Jing Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Transcription Factor ◽  
Liquid Chromatography ◽  
Zinc Finger ◽  
Leaf Senescence ◽  
Transcriptional Activity ◽  
Molecular Mechanisms ◽  
Liquid Chromatography Mass Spectrometry ◽  
Zinc Finger Transcription Factor ◽  
Insight Into

AbstractJasmonic acid (JA) plays an important role in regulating leaf senescence. However, the molecular mechanisms of leaf senescence in apple (Malus domestica) remain elusive. In this study, we found that MdZAT10, a C2H2-type zinc finger transcription factor (TF) in apple, markedly accelerates leaf senescence and increases the expression of senescence-related genes. To explore how MdZAT10 promotes leaf senescence, we carried out liquid chromatography/mass spectrometry screening. We found that MdABI5 physically interacts with MdZAT10. MdABI5, an important positive regulator of leaf senescence, significantly accelerated leaf senescence in apple. MdZAT10 was found to enhance the transcriptional activity of MdABI5 for MdNYC1 and MdNYE1, thus accelerating leaf senescence. In addition, we found that MdZAT10 expression was induced by methyl jasmonate (MeJA), which accelerated JA-induced leaf senescence. We also found that the JA-responsive protein MdBT2 directly interacts with MdZAT10 and reduces its protein stability through ubiquitination and degradation, thereby delaying MdZAT10-mediated leaf senescence. Taken together, our results provide new insight into the mechanisms by which MdZAT10 positively regulates JA-induced leaf senescence in apple.

A comparative study of RFID adoption patterns in retail

2021 ◽  
pp. 1-20
Author(s):  
Narges Kasiri ◽  
G. Scott Erickson ◽  
Gerd Wolfram
Keyword(s):  
Comparative Study ◽  
Radio Frequency ◽  
Radio Frequency Identification ◽  
Structural Model ◽  
Slow Pace ◽  
The Us ◽  
Frequency Identification ◽  
Insight Into ◽  
Current Stage ◽  
Over Time

Radio frequency identification (RFID) has been viewed as a promising technology for quite some time. Initially developed a couple of decades ago, the technology has been accompanied by predictions of imminent widespread adoption since its beginnings. A majority of retailers and other users are now using or planning to use the technology. This paper employs a combination of the technology-organization-environment (TOE) model and the 3-S (substitution, scale, structural) model to analyze the long journey of RFID adoption in retail. Top retail executives in the US and Europe were interviewed to investigate RFID adoption patterns based on differences in technological, organizational, and environmental circumstances. As the retail industry is moving into a post-adoption era, these results demonstrate the current stage of retail RFID adoption, identify factors playing important roles over time as motivators or impediments, and provide some insight into the slow pace of adoption.

scholarly journals The role of phagocytosis-dependent activation of TFEB in the clearance of salmonella

2021 ◽  
Author(s):  
Erika Ospina Escobar
Keyword(s):  
Transcription Factor ◽  
Salmonella Typhimurium ◽  
Nuclear Translocation ◽  
Steady Increase ◽  
Phagosome Maturation ◽  
Salmonella Infection ◽  
Complex Interplay ◽  
Transcription Factor Eb ◽  
Insight Into

During phagocytosis, macrophages engulf and sequester pathogens into phagosomes. Phagosomes then fuse with acidic and degradative lysosomes to degrade the internalized pathogen. We previously demonstrated that phagocytosis of IgG-opsonized particles and non-opsonized E.coli causes activation of the Transcription Factor EB (TFEB), which enhances the expression of lysosomal genes, increases the degradative capacity of lysosomes and boosts bactericidal activity. However, pathogens like Salmonella typhimurium have evolved mechanisms to evade and/or alter phagosome maturation to promote their own survival. We investigated: i) whether pathogens like Salmonella can alter TFEB activation and ii) whether phagocytosis-dependent activation of TFEB can counteract the pathogenicity of microorganisms. Here, we show that non-viable (heat-killed) S. typhimurium, pathogenic (EHEC and UPEC) and non-pathogenic E.coli (DH5α) all caused TFEB nuclear translocation in RAW macrophages, while strikingly live S. typhimurium maintained TFEB in the cytosol in the first hours post-infection. By contrast, Salmonella mutants for ΔsifA, ΔsopD2, ΔphoP all triggered TFEB activation in the first hour of infection. However, Salmonella infection eventually triggered a steady increase in nuclear TFEB after 4 h of infection, suggesting a more complex interplay between TFEB and Salmonella infection. We dissected the importance of TFEB activation towards Salmonella survivability by pre-activating TFEB before infection within WT macrophages and macrophages with a CRISPR-based deletion of TFEB. Our work suggests that Salmonella actively interferes with TFEB signaling in order to enhance its own survival. These results could provide insight into using TFEB as a target for the clearance of infections.

scholarly journals Molecular characterization of a complex of apoptosis-inducing factor 1 with cytochrome c oxidase of the mitochondrial respiratory chain

2021 ◽  
Vol 118 (39) ◽  
pp. e2106950118
Author(s):  
Johannes F. Hevler ◽  
Riccardo Zenezeni Chiozzi ◽  
Alfredo Cabrera-Orefice ◽  
Ulrich Brandt ◽  
Susanne Arnold ◽  
...  
Keyword(s):  
Oxidative Phosphorylation ◽  
Respiratory Chain ◽  
Structural Model ◽  
Direct Electron Transfer ◽  
Mitochondrial Respiratory Chain ◽  
Structural Restraints ◽  
Apoptosis Inducing Factor ◽  
Insight Into ◽  
Macromolecular Organization

Combining mass spectrometry–based chemical cross-linking and complexome profiling, we analyzed the interactome of heart mitochondria. We focused on complexes of oxidative phosphorylation and found that dimeric apoptosis-inducing factor 1 (AIFM1) forms a defined complex with ∼10% of monomeric cytochrome c oxidase (COX) but hardly interacts with respiratory chain supercomplexes. Multiple AIFM1 intercross-links engaging six different COX subunits provided structural restraints to build a detailed atomic model of the COX-AIFM12 complex (PDBDEV_00000092). An application of two complementary proteomic approaches thus provided unexpected insight into the macromolecular organization of the mitochondrial complexome. Our structural model excludes direct electron transfer between AIFM1 and COX. Notably, however, the binding site of cytochrome c remains accessible, allowing formation of a ternary complex. The discovery of the previously overlooked COX-AIFM12 complex and clues provided by the structural model hint at potential roles of AIFM1 in oxidative phosphorylation biogenesis and in programmed cell death.

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