scholarly journals Multidimensional Separation Using HILIC and SCX Pre-fractionation for RP LC-MS/MS Platform with Automated Exclusion List-based MS Data Acquisition with Increased Protein Quantification

2015 ◽  
Vol 8 (11) ◽  
Author(s):  
Yu Zhou ◽  
Zhen Meng ◽  
Maria Edman-Woolcott
2018 ◽  
Author(s):  
Cheng Chang ◽  
Zhiqiang Gao ◽  
Wantao Ying ◽  
Yan Zhao ◽  
Yan Fu ◽  
...  

AbstractMass spectrometry (MS) has become a prominent choice for large-scale absolute protein quantification, but its quantification accuracy still has substantial room for improvement. A crucial issue is the bias between the peptide MS intensity and the actual peptide abundance, i.e., the fact that peptides with equal abundance may have different MS intensities. This bias is mainly caused by the diverse physicochemical properties of peptides. Here, we propose a novel algorithm for label-free absolute protein quantification, LFAQ, which can correct the biased MS intensities by using the predicted peptide quantitative factors for all identified peptides. When validated on datasets produced by different MS instruments and data acquisition modes, LFAQ presented accuracy and precision superior to those of existing methods. In particular, it reduced the quantification error by an average of 46% for low-abundance proteins.


1990 ◽  
Vol 51 (C2) ◽  
pp. C2-939-C2-942 ◽  
Author(s):  
N. DINER ◽  
A. WEILL ◽  
J. Y. COAIL ◽  
J. M. COUDEVILLE

2010 ◽  
Vol 6 (2) ◽  
pp. 43 ◽  
Author(s):  
Andreas H Mahnken ◽  

Over the last decade, cardiac computed tomography (CT) technology has experienced revolutionary changes and gained broad clinical acceptance in the work-up of patients suffering from coronary artery disease (CAD). Since cardiac multidetector-row CT (MDCT) was introduced in 1998, acquisition time, number of detector rows and spatial and temporal resolution have improved tremendously. Current developments in cardiac CT are focusing on low-dose cardiac scanning at ultra-high temporal resolution. Technically, there are two major approaches to achieving these goals: rapid data acquisition using dual-source CT scanners with high temporal resolution or volumetric data acquisition with 256/320-slice CT scanners. While each approach has specific advantages and disadvantages, both technologies foster the extension of cardiac MDCT beyond morphological imaging towards the functional assessment of CAD. This article examines current trends in the development of cardiac MDCT.


2020 ◽  
Vol E103.C (8) ◽  
pp. 345-352
Author(s):  
Zhongyuan ZHOU ◽  
Mingjie SHENG ◽  
Peng LI ◽  
Peng HU ◽  
Qi ZHOU

2020 ◽  
Vol 65 (1) ◽  
pp. 179-186
Author(s):  
Mihaela Dorica Stroia

Current software development directions open up a world of possibilities, especially in the engineering field. Present paper is meant to highlight the advantages and in particular the ease of using virtual instrumentation facilities, with a proper and adequate design and implementation of desired instrument. In this idea we bring into discussion a design for virtual instrument which can be used for data acquisition that can be stored for further simulations according to the needs required by the process in discussion.


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