scholarly journals Systematic Analysis of Yeast Proteome Reveals Peptide Detectability Factors for Mass Spectrometry

2015 ◽  
Vol 8 (10) ◽  
Author(s):  
Sunhee Jung Samuel A ◽  
Danziger Alexandre ◽  
Panchaud Priska von Haller
Keyword(s):  
Mass Spectrometry ◽  
Systematic Analysis ◽  
Yeast Proteome ◽  
Peptide Detectability

Advance of Peptide Detectability Prediction on Mass Spectrometry Platform in Proteomics

2010 ◽  
Vol 38 (2) ◽  
pp. 286-292 ◽  
Author(s):  
Chang-Ming XU ◽  
Ji-Yang ZHANG ◽  
Hui LIU ◽  
Han-Chang SUN ◽  
Yun-Ping ZHU ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Peptide Detectability

scholarly journals Development of a Clinical MALDI-ToF Mass Spectrometry Assay for SARS-CoV-2: Rational Design and Multi-Disciplinary Team Work

Diagnostics ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 746 ◽  
Author(s):  
Ray K. Iles ◽  
Raminta Zmuidinaite ◽  
Jason K. Iles ◽  
George Carnell ◽  
Alex Sampson ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Immune Response ◽  
Rational Design ◽  
Viral Envelope ◽  
Team Work ◽  
Diagnostic Process ◽  
Team Approach ◽  
Systematic Analysis ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

The COVID-19 pandemic caused by the SARS-CoV-2 coronavirus has stretched national testing capacities to breaking points in almost all countries of the world. The need to rapidly screen vast numbers of a country’s population in order to control the spread of the infection is paramount. However, the logistical requirement for reagent supply (and associated cost) of RT-PCR based testing (the current front-line test) have been hugely problematic. Mass spectrometry-based methods using swab and gargle samples have been reported with promise, but have not approached the task from a systematic analysis of the entire diagnostic process. Here, the pipeline from sample processing, the biological characteristics of the pathogen in human biofluid, the downstream bio- and physical-chemistry and the all-important data processing with clinical interpretation and reporting, are carefully compiled into a single high-throughput and reproducible rapid process. Utilizing MALDI-ToF mass spectrometric detection to viral envelope glycoproteins in a systems biology-multidisciplinary team approach, we have achieved a multifaceted clinical MALDI ToF MS screening test, primarily (but not limited to) SARS-CoV-2, with direct application to other future epidemics/pandemics that may arise. The clinical information generated not only includes SARS-CoV-2 coronavirus detection–(Spike protein fragments S1, S2b, S2a peaks), but other respiratory viral infections detected as well as an assessment of generalised oral upper respiratory immune response (elevated total Ig light chain peak) and a measure of the viral immune response (elevated intensity of IgA heavy chain peak). The advantages of the method include; (1) ease of sampling, (2) speed of analysis, and much reduced cost of testing. These features reveal the diagnostic utility of MALDI-ToF mass spectrometry as a powerful and economically attractive global solution

scholarly journals Robust Analysis of the Yeast Proteome under 50 kDa by Molecular-Mass-Based Fractionation and Top-Down Mass Spectrometry

2011 ◽  
Vol 84 (1) ◽  
pp. 209-215 ◽  
Author(s):  
John F. Kellie ◽  
Adam D. Catherman ◽  
Kenneth R. Durbin ◽  
John C. Tran ◽  
Jeremiah D. Tipton ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Mass ◽  
Top Down ◽  
Robust Analysis ◽  
Yeast Proteome ◽  
Top Down Mass Spectrometry
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