Combining Proteomic Strategies and Molecular Display Technology for Development of Vaccines against Candida albicans

2014 ◽  
Vol 07 (06) ◽  
Author(s):  
Seiji Shibasaki Miki Karasaki
Keyword(s):  
Candida Albicans ◽  
Molecular Display ◽  
Display Technology

scholarly journals Bioadsorption Strategies with Yeast Molecular Display Technology

2014 ◽  
Vol 19 (4) ◽  
pp. 157-164 ◽  
Author(s):  
SEIJI SHIBASAKI ◽  
MITSUYOSHI UEDA
Keyword(s):  
Molecular Display ◽  
Display Technology

scholarly journals Generation and Characterization of Single Chain Variable Fragment against Alpha-Enolase of Candida albicans

2020 ◽  
Vol 21 (8) ◽  
pp. 2903
Author(s):  
Sy-Jye Leu ◽  
Yu-Ching Lee ◽  
Chi-Hsin Lee ◽  
Po-Yen Liao ◽  
Chen-Wei Chiang ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Animal Studies ◽  
New Drugs ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Candida Spp ◽  
Phage Display Technology ◽  
Multifunctional Protein ◽  
Display Technology ◽  
Opportunistic Human Pathogen

Candida albicans (C. albicans) is an opportunistic human pathogen responsible for approximately a half of clinical candidemia. The emerging Candida spp. with resistance to azoles is a major challenge in clinic, suggesting an urgent demand for new drugs and therapeutic strategies. Alpha–enolase (Eno1) is a multifunctional protein and represents an important marker for invasive candidiasis. Thus, C. albicans Eno1 (CaEno1) is believed to be an important target for the development of therapeutic agents and antibody drugs. Recombinant CaEno1 (rCaEno1) was first used to immunize chickens. Subsequently, we used phage display technology to construct two single chain variable fragment (scFv) antibody libraries. A novel biopanning procedure was carried out to screen anti-rCaEno1 scFv antibodies, whose specificities were further characterized. The polyclonal IgY antibodies showed binding to rCaEno1 and native CaEno1. A dominant scFv (CaS1) and its properties were further characterized. CaS1 attenuated the growth of C. albicans and inhibited the binding of CaEno1 to plasminogen. Animal studies showed that CaS1 prolonged the survival rate of mice and zebrafish with candidiasis. The fungal burden in kidney and spleen, as well as level of inflammatory cytokines were significantly reduced in CaS1-treated mice. These results suggest CaS1 has potential of being immunotherapeutic drug against C. albicans infections.

Artificial peptides binding to the c face of hydroxyapatite obtained by molecular display technology

RSC Advances ◽  
2013 ◽  
Vol 3 (6) ◽  
pp. 1885-1889 ◽  
Author(s):  
Shohei Ono ◽  
Toru Tsuji ◽  
Yuya Oaki ◽  
Hiroaki Imai
Keyword(s):  
Molecular Display ◽  
Display Technology

scholarly journals Molecular Display Technology Using Yeast—Arming Technology—

2009 ◽  
Vol 25 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Seiji SHIBASAKI ◽  
Hatsuo MAEDA ◽  
Mitsuyoshi UEDA
Keyword(s):  
Molecular Display ◽  
Display Technology

Enhanced IgE response to Candida albicans in postoperative invasive candidiasis

1996 ◽  
Vol 26 (4) ◽  
pp. 452-460 ◽  
Author(s):  
J. SAVOLAINEN ◽  
A. RANTALA ◽  
M. NERMES ◽  
L. LEHTONEN ◽  
M. VIANDER
Keyword(s):  
Candida Albicans ◽  
Invasive Candidiasis ◽  
Ige Response

Existence of Candida albicans and microorganisms in denture stomatitis patients

1997 ◽  
Vol 24 (10) ◽  
pp. 788-790 ◽  
Author(s):  
Y. KULAK ◽  
A. ARIKAN ◽  
E. KAZAZOGLU
Keyword(s):  
Candida Albicans ◽  
Denture Stomatitis
Sign in / Sign up

Export Citation Format

Share Document