Molecular Cloning of MAP Kinase Genes and In silico Identification of their Downstream Transcription Factors Involved in Pathogenesis of Karnal bunt(Tilletia indica) of Wheat

2011 ◽  
Vol 04 (08) ◽  
Author(s):  
Atul K Gupta Anshita Goel
Keyword(s):  
Transcription Factors ◽  
Map Kinase ◽  
Molecular Cloning ◽  
In Silico ◽  
Karnal Bunt ◽  
Tilletia Indica ◽  
In Silico Identification

scholarly journals Induction of MAP Kinase Homologues during Growth and Morphogenetic Development of Karnal Bunt (Tilletia indica) under the Influence of Host Factor(s) from Wheat Spikes

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Atul K. Gupta ◽  
J. M. Seneviratne ◽  
G. K. Joshi ◽  
Anil Kumar
Keyword(s):  
Map Kinase ◽  
Map Kinases ◽  
Fungal Growth ◽  
Host Factor ◽  
Host Factors ◽  
Karnal Bunt ◽  
Dependent Manner ◽  
Tilletia Indica ◽  
Mitogen Activated Protein

Signaling pathways that activate different mitogen-activated protein kinases (MAPKs) in response to certain environmental conditions, play important role in mating type switching (Fus3) and pathogenicity (Pmk1) in many fungi. In order to determine the roles of such regulatory genes inTilletia indica, the causal pathogen of Karnal bunt (KB) of wheat, semi-quantitative and quantitative RT-PCR was carried out to isolate and determine the expression of MAP kinase homologues during fungal growth and development underin vitroculture. Maximum expression of TiFus3 and TiPmk1 genes were observed at 14th and 21st days of culture and decreased thereafter. To investigate whether the fungus alters the expression levels of same kinases upon interaction with plants, cultures were treated with 1% of host factors (extracted from S-2 stage of wheat spikes). Such treatment induced the expression of MAPks in time dependent manner compared to the absence of host factors. These results suggest that host factor(s) provide certain signal(s) which activate TiFus3 and TiPmk1 during morphogenetic development ofT. indica. The results also provides a clue about the role of host factors in enhancing the disease potential due to induction of MAP kinases involved in fungal development and pathogenecity.

scholarly journals Expression and in silico characterization of Phenylalanine ammonium lyase against karnal bunt (Tilletia indica) in wheat (Triticum aestivum)

2013 ◽  
Vol 9 (20) ◽  
pp. 1013-1018 ◽  
Author(s):  
Shalini Purwar ◽  
◽  
Shanthy Sundaram ◽  
Sukrat Sinha ◽  
Ankit Gupta ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
In Silico ◽  
Karnal Bunt ◽  
Tilletia Indica ◽  
In Silico Characterization

scholarly journals Four In Silico Designed and Validated qPCR Assays to Detect and Discriminate Tilletia indica and T. walkeri, Individually or as a Complex

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1295
Author(s):  
Émilie D. Tremblay ◽  
Julie Carey ◽  
Guillaume J. Bilodeau ◽  
Sarah Hambleton
Keyword(s):  
In Silico ◽  
Single Copy ◽  
Karnal Bunt ◽  
Grass Species ◽  
Tilletia Indica ◽  
Recent Approach ◽  
Highly Sensitive ◽  
Close Relatives ◽  
High Level ◽  
Genomic Regions

Several fungi classified in the genus Tilletia are well-known to infect grass species including wheat (Triticum). Tilletia indica is a highly unwanted wheat pathogen causing Karnal bunt, subject to quarantine regulations in many countries. Historically, suspected Karnal bunt infections were identified by morphology, a labour-intensive process to rule out other tuberculate-spored species that may be found as contaminants in grain shipments, and the closely-related pathogen T. walkeri on ryegrass (Lolium). Molecular biology advances have brought numerous detection tools to discriminate Tilletia congeners (PCR, qPCR, etc.). While those tests may help to identify T. indica more rapidly, they share weaknesses of targeting insufficiently variable markers or lacking sensitivity in a zero-tolerance context. A recent approach used comparative genomics to identify unique regions within target species, and qPCR assays were designed in silico. This study validated four qPCR tests based on single-copy genomic regions and with highly sensitive limits of detection (~200 fg), two to detect T. indica and T. walkeri separately, and two newly designed, targeting both species as a complex. The assays were challenged with reference DNA of the targets, their close relatives, other crop pathogens, the wheat host, and environmental specimens, ensuring a high level of specificity for accurate discrimination.

Sign in / Sign up

Export Citation Format

Share Document