Molecular Basis of Chemical Chaperone Effects of N-octyl-b-valienamine on Human b-glucosidase in Low/neutral pH Conditions

2010 ◽  
Vol 03 (04) ◽  
pp. 104-112 ◽  
Author(s):  
Hiroyuki Jo ◽  
Katsuyuki Yugi ◽  
Seiichiro Ogawa ◽  
Yoshiyuki Suzuki ◽  
Yasubumi Sakakibara
Keyword(s):  
Molecular Basis ◽  
Chemical Chaperone ◽  
Neutral Ph ◽  
Ph Conditions

Influence of bend geometry on flow accelerated corrosion under neutral pH conditions

2021 ◽  
Vol 122 ◽  
pp. 105127
Author(s):  
P. Madasamy ◽  
M. Mukunthan ◽  
P. Chandramohan ◽  
T.V. Krishna Mohan ◽  
Andrews Sylvanus ◽  
...  
Keyword(s):  
Accelerated Corrosion ◽  
Neutral Ph ◽  
Ph Conditions ◽  
Flow Accelerated Corrosion

scholarly journals Synthesis and HNO Donating Properties of the Piloty's Acid Analogue Trifluoromethanesulphonylhydroxamic Acid: Evidence for Quantitative Release of HNO at Neutral pH Conditions

2018 ◽  
Vol 24 (29) ◽  
pp. 7330-7334 ◽  
Author(s):  
Sonya K. Adas ◽  
Vinay Bharadwaj ◽  
Yang Zhou ◽  
Jiuhong Zhang ◽  
Alexander J. Seed ◽  
...  
Keyword(s):  
Neutral Ph ◽  
Acid Analogue ◽  
Ph Conditions

Transcriptional profiling reveals molecular basis and the role of arginine in response to low-pH stress in Pichia kudriavzevii

2019 ◽  
Author(s):  
Hao Ji ◽  
Xiameng Dong ◽  
Kailun Zhang ◽  
Libo Jin ◽  
Renyi Peng ◽  
...  
Keyword(s):  
Organic Acids ◽  
Molecular Basis ◽  
Transcriptional Profiling ◽  
Cell Aggregation ◽  
Membrane Lipid ◽  
Low Ph ◽  
Pichia Kudriavzevii ◽  
Ph Stress ◽  
Ph Conditions

Abstract BackgroundThe non-conventional yeast Pichia kudriavzevii possesses a unique ability to tolerate various environmental stresses particularly low-pH stress. Thus, it is considered to be a promising biotechnological host for the production of various organic acids under low-pH conditions. However, little is known about the low-pH stress response in P. kudriavzevii, which significantly restricts its future development. ResultsIn this study, P. kudriavzevii JLY1107 showed great tolerance to low-pH stress, but its cell aggregation upon acidic conditions is unfavorable for the development of low-pH fermentation. To explore the molecular basis, we conducted RNA-Seq to compare global gene expression in response to low-pH. Among the 429 differentially expressed genes, the genes associated with regulation of membrane lipid composition, filamentous growth and arginine metabolism were selected for in-depth discussions. The up-regulation of genes associated with arginine uptake and degradation suggests a potential role of arginine in response to low-pH strsss. We therefore present data supporting the hypothesis that P. kudriavzevii maintains intracellular homeostasis by using the ammonia produced by arginine catabolism. Furthermore, external addition of arginine significantly enhances growth and reduces cell aggregation of P. kudriavzevii under low-pH conditions.ConclusionsArginine was demonstrated to be a promising molecule for improving cell growth and preventing cell aggregation under extremely low-pH conditions. Our study is a step towards developing the non-conventional yeast P. kudriavzevii as a platform host for the production of organic acids under low-pH conditions.

scholarly journals Improved Phos-tag SDS-PAGE under neutral pH conditions for advanced profiling of protein phosphorylation

2012 ◽  
Vol 56 (Suppl_1) ◽  
pp. s41-s44
Author(s):  
Emiko Kinoshita-Kikuta ◽  
Eiji Kinoshita ◽  
Tohru Koike
Keyword(s):  
Protein Phosphorylation ◽  
Neutral Ph ◽  
Sds Page ◽  
Ph Conditions

scholarly journals Epimerization and Decomposition of Kojibiose and Sophorose by Heat Treatment under Neutral pH Conditions

2019 ◽  
Vol 66 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Kazuhiro Chiku ◽  
Mami Wada ◽  
Haruka Atsuji ◽  
Arisa Hosonuma ◽  
Mitsuru Yoshida ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Neutral Ph ◽  
Ph Conditions

scholarly journals In situ observation of pH change during water splitting in neutral pH conditions: impact of natural convection driven by buoyancy effects

2020 ◽  
Vol 13 (12) ◽  
pp. 5104-5116
Author(s):  
Keisuke Obata ◽  
Roel van de Krol ◽  
Michael Schwarze ◽  
Reinhard Schomäcker ◽  
Fatwa F. Abdi
Keyword(s):  
Natural Convection ◽  
Water Splitting ◽  
In Situ Observation ◽  
Ph Change ◽  
Neutral Ph ◽  
Ph Conditions

Buoyancy-driven natural convection stabilizes the pH and reduces overpotentials during water splitting, both in near-neutral pH unbuffered and buffered solutions.

Microstructural Feature Analysis of X65 Steel Exposed to Ripple Load Testing Under Near Neutral pH Conditions

2014 ◽  
Author(s):  
Pellumb Jakupi ◽  
Bill Santos ◽  
Wilfred Binns ◽  
Ivan Barker ◽  
Jenny Been
Keyword(s):  
Stress Corrosion ◽  
Stress Corrosion Cracking ◽  
Pipeline Steel ◽  
Open Circuit ◽  
Electron Back Scatter Diffraction ◽  
Corrosion Cracking ◽  
Load Testing ◽  
Neutral Ph ◽  
X65 Steel ◽  
Ph Conditions

Newly designed miniature Compact Tension (CT) specimens, designed according to standard ASTM dimension ratios, and machined out of previously in-service X65 pipeline steel were exposed to super-imposed cyclic loading at high mean stresses in NS4 solution to determine the behaviour of X65 steel to ripple loading under near neutral pH conditions. Electron Back-Scatter Diffraction (EBSD) was used to study the microstructural grain geometry to determine if it influences stress-corrosion cracking (SCC) initiation and propagation. Prior to ripple load testing, finely polished X65 surfaces were subjected to EBSD measurements to characterize the microstructure’s geometry; i.e., grain and grain boundary orientations and texture. On the same locations where EBSD maps were recorded, a grid of cross-shaped resist markings — approximately 1–5 μm in size — were deposited every 15 μm across the analyzed surfaces. Following microscopic analyses the specimens were pre-cracked and re-examined to determine whether the crack initiation procedure preconditions the residual strain (quantified by grain misorientations) around an induced crack. Then, ripple load testing at stress levels characterized by load ratios (R) greater than 0.9 was performed, while simultaneously monitoring the open-circuit potential (OCP) at room temperature. The originally characterized surface was again re-examined to determine if the crack tip propagated preferably along a specific crystallographic grain orientation by comparing the shifts in each cross-shaped grid. Results from this investigation will help determine if there is a link between microstructural grain geometries and transgranular stress corrosion cracking.

Sign in / Sign up

Export Citation Format

Share Document