Epidemiological Survey of Hantaan Virus Infection of Wild Rodents Trapped in Jeollanam-do, 2003~2004

2006 ◽  
Vol 36 (3) ◽  
pp. 205 ◽  
Author(s):  
Hyeon-Je Song ◽  
Dae-Yeon Lee ◽  
Choong-Mo Kim ◽  
Young-Hack Shin
Keyword(s):  
Virus Infection ◽  
Epidemiological Survey ◽  
Hantaan Virus ◽  
Wild Rodents

scholarly journals A Case of Graves' Disease Combined with Hantaan Virus Infection

2009 ◽  
Vol 24 (1) ◽  
pp. 158 ◽  
Author(s):  
Heung Yong Jin ◽  
Seon Mee Kang ◽  
So Young Kim ◽  
Ji Hyun Park ◽  
Hong Sun Baek ◽  
...  
Keyword(s):  
Virus Infection ◽  
Hantaan Virus ◽  
Graves Disease

Acute Appendicitis Associated with Hantaan Virus Infection

2021 ◽  
Author(s):  
Sung-Chul Lim ◽  
Young Min Lee ◽  
Choon-Mee Kim ◽  
Na Ra Yun ◽  
Dong-Min Kim
Keyword(s):  
Virus Infection ◽  
Peripheral Nerve ◽  
Hemorrhagic Fever ◽  
Hantaan Virus ◽  
Nerve Bundle ◽  
Rt Pcr ◽  
Antibody Assay ◽  
Immunofluorescent Antibody ◽  
Virus Antigens ◽  
Indirect Immunofluorescent

Hantaviruses are Bunyaviridae viruses that cause hemorrhagic fever with renal syndrome (HFRS). Appendicitis caused by Hantaan virus has not been reported previously. An 81-year-old man who underwent laparoscopic appendectomy for suspected appendicitis based on abdominal pain, fever, hypotension, and computed tomography findings. Based on a suspicion of hemorrhagic fever with renal syndrome, the patient’s plasma was simultaneously analyzed using an indirect immunofluorescent antibody assay and nested reverse transcription–polymerase chain reaction (RT-PCR). The appendix tissue was also analyzed using nested RT-PCR and immunohistochemical (IHC) staining to identify the presence of Hantaan virus. Nested RT-PCR detected the presence of Hantaan virus, and indirect immunofluorescent antibody assay results revealed the presence of elevated antibody levels. Furthermore, IHC staining of the appendix tissue confirmed Hantaan virus antigens in the peripheral nerve bundle. Based on these findings, we confirmed the nerve tropism of the Hantaan virus. Hantaan virus in plasma and appendix tissue samples was confirmed using PCR and phylogenetic tree analysis. Moreover, we detected hypertrophy of the submucosa and periappendiceal adipose tissue nerve bundle along with Hantaan virus antigens in peripheral nerve bundles using IHC staining. Hence, we report that Hantaan virus infection may be accompanied by appendicitis.

scholarly journals Serologic Diagnosis of Hantaan Virus Infection Based on a Peptide Antigen

2002 ◽  
Vol 48 (4) ◽  
pp. 645-647 ◽  
Author(s):  
Zheng Li ◽  
Xuefan Bai ◽  
Huijie Bian
Keyword(s):  
Virus Infection ◽  
Hantaan Virus ◽  
Peptide Antigen ◽  
Serologic Diagnosis

scholarly journals Isolation of Hokkaido virus, genus Hantavirus, using a newly established cell line derived from the kidney of the grey red-backed vole (Myodes rufocanus bedfordiae)

2012 ◽  
Vol 93 (10) ◽  
pp. 2237-2246 ◽  
Author(s):  
Takahiro Sanada ◽  
Takahiro Seto ◽  
Yuka Ozaki ◽  
Ngonda Saasa ◽  
Kumiko Yoshimatsu ◽  
...  
Keyword(s):  
Cell Line ◽  
Culture Fluid ◽  
Natural Host ◽  
Hantaan Virus ◽  
Myodes Glareolus ◽  
Established Cell Line ◽  
Wild Rodents ◽  
African Green Monkey Kidney ◽  
A Cell ◽  
Myodes Rufocanus

Hantaviruses belong to the family Bunyaviridae and are maintained in wild rodents. Although Vero E6 cells, which originate from African green monkey kidney, are used widely in hantavirus research, isolation of hantaviruses from this cell line is difficult. To develop an efficient method of propagation and isolation of hantaviruses we established a novel cell line, MRK101, derived from the kidney of the grey red-backed vole (Myodes rufocanus bedfordiae), the natural host of Hokkaido virus (HOKV). The MRK101 cells showed a significantly higher susceptibility to Puumala virus (PUUV) hosted by Myodes glareolus than Vero E6 cells. Viral nucleocapsid protein in PUUV-infected MRK101 cells was detected earlier than in Vero E6 cells, and the viral titre in the culture fluid of MRK101 cells was higher than that of Vero E6 cells during the early phase of infection. In contrast, MRK101 cells showed no susceptibility to Hantaan virus. HOKV, which has not been isolated to date, was isolated successfully using MRK101 cells. Moreover, the newly isolated HOKV was successfully propagated in MRK101, but not Vero E6, cells. Phylogenic analyses of the S (small), M (medium) and L (large) segment sequences revealed that HOKV is related most closely to PUUV, but is distinct from other hantaviruses. These data suggest that the MRK101 cell line is a useful tool for the isolation and propagation of hantaviruses. Moreover, this is (to our knowledge) the first report of hantavirus isolation in a cell line that originated from the natural host.

Effect of MDP-Lys(L18), a derivative of MDP, on enhancing host resistance against Hantaan virus infection in newborn mice

Vaccine ◽  
1995 ◽  
Vol 13 (14) ◽  
pp. 1300-1305 ◽  
Author(s):  
Yung Choon Yoo ◽  
Kumiko Yoshimatsu ◽  
Rei Hatsuse ◽  
Mizuho Tamura ◽  
Ryu Yoshida ◽  
...  
Keyword(s):  
Virus Infection ◽  
Host Resistance ◽  
Hantaan Virus ◽  
Newborn Mice
Sign in / Sign up

Export Citation Format

Share Document