scholarly journals Some properties and host range of a new strain of soybean mosaic virus isolated from soybean seedlings

2013 ◽  
Vol 55 (0) ◽  
pp. 61-64
Author(s):  
Shigemitsu Kimura ◽  
Naoko Kawato ◽  
Kazunobu Okadome ◽  
Hisashi Amano
Keyword(s):  
Host Range ◽  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
Soybean Seedlings

scholarly journals Soybean mosaic virus: a successful potyvirus with a wide distribution but restricted natural host range

2018 ◽  
Vol 19 (7) ◽  
pp. 1563-1579 ◽  
Author(s):  
M. R. Hajimorad ◽  
L. L. Domier ◽  
S. A. Tolin ◽  
S. A. Whitham ◽  
M. A. Saghai Maroof
Keyword(s):  
Host Range ◽  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
Wide Distribution ◽  
Natural Host ◽  
Natural Host Range

scholarly journals Host Range Studies of Soybean Mosaic Virus

2017 ◽  
Vol 6 (7) ◽  
pp. 304-308
Author(s):  
H.V. Nandakishor ◽  
B. Kumaraswamy ◽  
S.S. Mane ◽  
G. Amrutha Veena
Keyword(s):  
Host Range ◽  
Mosaic Virus ◽  
Soybean Mosaic Virus

scholarly journals First Report of Bean pod mottle virus in Soybean in Ohio

Plant Disease ◽  
2001 ◽  
Vol 85 (9) ◽  
pp. 1029-1029 ◽  
Author(s):  
A. E. Dorrance ◽  
D. T. Gordon ◽  
A. F. Schmitthenner ◽  
C. R. Grau
Keyword(s):  
Host Range ◽  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
Mottle Virus ◽  
Yellow Mosaic Virus ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bean Pod Mottle Virus ◽  
First Report ◽  
Soybean Leaf

Soybean has been increasing in importance and acreage over wheat and corn for the past decade in Ohio and is now planted on 4.5 million acres. Previous surveys in Ohio of viruses infecting soybean failed to identify Bean pod mottle virus (BPMV) and soybean virus diseases have rarely caused economic losses (1). During 1999, producers in Ohio noticed virus-like symptoms in soybeans in a few isolated locations. Soybeans with green stems, undersized and “turned up pods” were collected from Union, Wood and Wyandot Counties during October 1999 and soybeans with crinkled, mottled leaves were collected in Henry, Licking and Sandusky during August 2000. Five to six plants were collected from a single field from each county each year. In 1999, samples were sent to the University of Wisconsin-Madison, where one symptomatic leaflet/sample was ground in 3 ml of chilled phosphate buffered saline (pH 7.2). Leaf sap was placed in 1.5-ml centrifuge tubes and stored at 4°C for 24 h. Sap was assayed for the presence of BPMV using an alkaline phosphatase-labeled double-antibody sandwich enzyme-linked immunosorbent assay (DAS ELISA) for BPMV (AgDia Inc., Elkhart, IN). All samples tested were positive for BPMV. Samples collected in 1999 were also maintained at The Ohio State University in Harosoy soybean and in 2000 assayed serologically along with samples collected in 2000 for BPMV and Soybean mosaic virus (SMV) by ELISA and for Tobacco ringspot virus (TRSV) and Bean yellow mosaic virus (BYMV) by a host-range symptom assay; SMV, BYMV and TRSV had been identified from soybean in previous Ohio surveys. Soybean leaf samples were assayed using F(ab′)2-Protein A ELISA with antiserum prepared in 1968 to a southern U.S. isolate of BPMV and to an Ohio isolate of Soybean mosaic virus (SMV) prepared in 1967, both stored at −20°C. Diseased and non-symptomatic soybean leaf samples were ground in 4 ml 0.025M Tris pH 8.0, 0.015M NaCl and 0.05% Tween 20. Extracts were tested for BPMV and SMV by ELISA following a protocol described elsewhere (2). All of the samples collected during 1999 and maintained in the greenhouse tested positive for both BPMV and SMV while all of those samples collected during 2000 tested positive for BPMV and negative for SMV. Host-range symptom assays were conducted with leaf extracts prepared by grinding 1 g tissue:10 ml potassium phosphate buffer, pH 7.0. Extracts were inoculated by leaf rub method to Harosoy soybean, Phaseolus vulgaris cvs. Red Kidney and Bountiful, cowpea, and cucumber. The host-range symptom assays of both the 1999 and 2000 samples were negative for TRSV and BYMV; cowpea failed to express local lesions and cucumber systemic mosaic characteristic of TRSV infection and the two Phaseolus cultivars the yellow mosaic characteristic of BYMV infection. These results indicate that both BPMV and SMV were present in the samples in 1999 but only BPMV in 2000. The distribution of BPMV within Ohio and economic impact of this virus have yet to be determined. This is the first report of BPMV in Ohio. References: (1) A. F. Schmitthenner and D. T. Gordon. Phytopathology 59:1048, 1969. (2) R. Louie et al. Plant Dis. 84:1133–1139, 2000.

scholarly journals Precise Exchange of the Helper-Component Proteinase Cistron Between Soybean mosaic virus and Clover yellow vein virus: Impact on Virus Viability and Host Range Specificity

2020 ◽  
Vol 110 (1) ◽  
pp. 206-214 ◽  
Author(s):  
Y. Wang ◽  
W. Xu ◽  
J. Abe ◽  
K. S. Nakahara ◽  
M. R. Hajimorad
Keyword(s):  
Host Range ◽  
Mosaic Virus ◽  
Broad Bean ◽  
Soybean Mosaic Virus ◽  
Wild Soybean ◽  
Systemic Infection ◽  
Yellow Vein ◽  
Clover Yellow Vein Virus ◽  
Helper Component Proteinase ◽  
Cultivated Soybean

Soybean mosaic virus and Clover yellow vein virus are two definite species of the genus Potyvirus within the family Potyviridae. Soybean mosaic virus-N (SMV-N) is well adapted to cultivated soybean (Glycine max) genotypes and wild soybean (G. soja), whereas it remains undetectable in inoculated broad bean (Vicia faba). In contrast, clover yellow vein virus No. 30 (ClYVV-No. 30) is capable of systemic infection in broad bean and wild soybean; however, it infects cultivated soybean genotypes only locally. In this study, SMV-N was shown to also infect broad bean locally; hence, broad bean is a host for SMV-N. Based on these observations, it was hypothesized that lack of systemic infection by SMV-N in broad bean and by ClYVV-No. 30 in cultivated soybean is attributable to the incompatibility of multifunctional helper-component proteinase (HC-Pro) in these hosts. The logic of selecting the HC-Pro cistron as a target is based on its established function in systemic movement and being a relevant factor in host range specificity of potyviruses. To test this hypothesis, chimeras were constructed with precise exchanges of HC-Pro cistrons between SMV-N and ClYVV-No. 30. Upon inoculation, both chimeras were viable in infection, but host range specificity of the recombinant viruses did not differ from those of the parental viruses. These observations suggest that (i) HC-Pro cistrons from SMV-N and ClYVV-No. 30 are functionally compatible in infection despite 55.6 and 48.9% nucleotide and amino acid sequence identity, respectively, and (ii) HC-Pro cistrons from SMV-N and ClYVV-No. 30 are not the determinants of host specificity on cultivated soybean or broad beans, respectively.

scholarly journals A New Potyvirus found in Passiflora incence in Florida

Plant Disease ◽  
2007 ◽  
Vol 91 (2) ◽  
pp. 227-227 ◽  
Author(s):  
C. A. Baker ◽  
L. Jones
Keyword(s):  
Coat Protein ◽  
Host Range ◽  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
Sodium Dodecyl ◽  
Chenopodium Quinoa ◽  
Bean Common Mosaic Virus ◽  
Plant Viruses ◽  
Genetic Sequencing ◽  
Cylindrical Inclusions

During March of 2004 (Alachua County) and again during February of 2006 (Highlands County), specimens of the plant Passiflora incence (passionfruit) with chlorotic symptoms were submitted to the Division of Plant Industry, Gainesville, FL for diagnosis. Cytoplasmic cylindrical inclusions seen in epidermal strips of plant leaves stained in Luxol brilliant green and calcomine orange but not seen in those stained in Azure A indicated the presence of a potyvirus infection. Leaf dips made for electron microscopy also showed virus particles consistent with a potyvirus infection. Reverse-transcription (RT)-PCR using degenerate potyvirus primers (4) produced a target ≈1.7-kb potyvirus band. Approximately 1.4 kb of the PCR fragments from both specimens was sequenced and was 100% identical. The 1.4-kb fragment contained the 3′ end of the NIb region, the coat protein, and the beginning of the 3′UTR. A GenBank BLAST search found that the two most similar potyviruses were Bean common mosaic necrosis virus (BCMNV), also known as serotype A of Bean common mosaic virus (BCMV), and Soybean mosaic virus (SMV). Molecular analysis of the 1.4-kb sequence using MegAlign (DNASTAR, Madison, WI) indicated a 73% identity with BCMNV and 68.8% with SMV. Analysis of the coat protein showed the highest identity (87%) with BCMNV, and for the NIb region, the highest identity occurred with SMV at 78%. In double-antibody sandwich (DAS)-ELISA, the virus did not react with antisera to either BCMV or BCMNV (BioReba). In sodium dodecyl sulfate (SDS)-immunodiffusion tests, however, the virus reacted heterologously with antiserum to Peanut stripe virus, now considered a member of the serotype B group of BCMV (1). In host range studies, this virus induced systemic chlorosis in Chenopodium quinoa but did not cause symptoms on any other host inoculated, including eight leguminous species and Nicotiana benthamiana. N. bethamiana is susceptible to BCMV and three other known Passiflora potyviruses, Passionfruit crinkle virus (PCV), Passionfruit woodiness virus (PWV), and Passionfruit mottle virus (PFMoV) (2,3). The cylindrical inclusions of this virus seen with the light microscope appeared as loosely aggregated medium length plate or needle-like structures as opposed to long, compact, bundle-shaped aggregates (PFMoV and PCV) or short plate-like structures (PWV) (2,3). The virus did not react with antisera (W. Zettler, University of Florida) to the three aforementioned passionfruit potyviruses in SDS-immunodiffusion tests. This virus, like PCV, PWV, and PFMoV, is related to the Bean common mosaic virus group. However, based on cylindrical inclusion morphology, host range, serology, and genetic sequencing, the virus appears to be a new potyvirus infecting Passiflora and is tentatively named Passiflora chlorosis virus. The sequence was deposited in GenBank as Accession No. DQ860147. References: (1) A. A Brunt et al. Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version 20, August 1996. (2) C. A. Chang. Phytopathology. 82:1358, 1992 (3) C. A. Chang et al. Plant Prot. Bull. 38:339, 1996. (4) A. Gibbs and A. Mackenzie. J. Virol. Methods 63:9, 1997.

Evaluation of germ plasm for resistant to Soybean mosaic virus (SMV)

2020 ◽  
Vol 21 (1) ◽  
pp. 6-9
Author(s):  
Wuye Ria Andayanie
Keyword(s):  
Abiotic Stress ◽  
Environmental Factors ◽  
Mosaic Virus ◽  
Polyclonal Antibody ◽  
Symptom Severity ◽  
Germ Plasm ◽  
Polyclonal Antibodies ◽  
Soybean Mosaic Virus ◽  
High Yield ◽  
High Yields

Soybean superior varieties with high yields and are resistant to abiotic stress have been largely released, although some varieties grown in the field are not resistant to SMV. In addition, the opportunity to obtain lines of hope as prospective varieties with high yield and resistance to SMV is very small. The method for evaluating soybean germplasm is based on serological observations of 98 accessions of leaf samples from SMV inoculation with T isolate. The evaluation results of 98 accessions based on visual observations showed 31 genotypes reacting very resistant or healthy to mild resistant category to SMV T isolate  with a percentage of symptom severity of 0 −30 %. Among 31 genotypes there are 2 genotypes (PI 200485; M8Grb 44; Mlg 3288) with the category of visually very resistant and resistant, respectively and  Mlg 3288  with the category of mild resistant.  They have a good agronomic appearance with a weight of 100 seeds (˃10 g) and react negatively with polyclonal antibodies to SMV, except Mlg 3288 reaction is not consistent, despite the weight of 100 seeds (˃ 10 g). Leaf samples from 98 accessions revealed various symptoms of SMV infection in the field. This diversity of symptoms is caused by susceptibility to accession, when infection occurs, and environmental factors. Keywords—: soybean; genotipe; Soybean mosaic virus (SMV); disease severity; polyclonal  antibody

Origin Analysis of Resistance Gene to Soybean Mosaic Virus in Soybean Line ICGR95-5117

2010 ◽  
Vol 36 (4) ◽  
pp. 549-554
Author(s):  
Rong-Xia GUAN ◽  
Yu-Bo CHEN ◽  
Hong-Liang FANG ◽  
Shuo LIU ◽  
Wei-Li TENG ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Soybean Mosaic Virus ◽  
Soybean Line ◽  
Origin Analysis

Inheritance of Reaction to Soybean Mosaic Virus in Two Soybean Cultivars

Crop Science ◽  
1989 ◽  
Vol 29 (6) ◽  
pp. 1439-1441 ◽  
Author(s):  
G. R. Buss ◽  
C. W. Roane ◽  
S. A. Tolin ◽  
P. Chen
Keyword(s):  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
Soybean Cultivars
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