scholarly journals Salicylate increases the expression of marA and reduces in vitro biofilm formation in uropathogenic Escherichia coli by decreasing type 1 fimbriae expression

Virulence ◽  
2012 ◽  
Vol 3 (3) ◽  
pp. 280-285 ◽  
Author(s):  
Jordi Vila ◽  
Sara M. Soto
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Uropathogenic Escherichia Coli ◽  
Type 1 Fimbriae

scholarly journals Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli

Microbiology ◽  
2005 ◽  
Vol 151 (10) ◽  
pp. 3287-3298 ◽  
Author(s):  
Caroline Blumer ◽  
Alexandra Kleefeld ◽  
Daniela Lehnen ◽  
Margit Heintz ◽  
Ulrich Dobrindt ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Invertible Element ◽  
Transcriptional Regulator ◽  
Host Cells ◽  
Yeast Cells ◽  
Type 1 Fimbriae ◽  
Biofilm Development

Type 1 fimbriae of Escherichia coli facilitate attachment to the host mucosa and promote biofilm formation on abiotic surfaces. The transcriptional regulator LrhA, which is known as a repressor of flagellar, motility and chemotaxis genes, regulates biofilm formation and expression of type 1 fimbriae. Whole-genome expression profiling revealed that inactivation of lrhA results in an increased expression of structural components of type 1 fimbriae. In vitro, LrhA bound to the promoter regions of the two fim recombinases (FimB and FimE) that catalyse the inversion of the fimA promoter, and to the invertible element itself. Translational lacZ fusions with these genes and quantification of fimE transcript levels by real-time PCR showed that LrhA influences type 1 fimbrial phase variation, primarily via activation of FimE, which is required for the ON-to-OFF transition of the fim switch. Enhanced type 1 fimbrial expression as a result of lrhA disruption was confirmed by mannose-sensitive agglutination of yeast cells. Biofilm formation was stimulated by lrhA inactivation and completely suppressed upon LrhA overproduction. The effects of LrhA on biofilm formation were exerted via the changed levels of surface molecules, most probably both flagella and type 1 fimbriae. Together, the data show a role for LrhA as a repressor of type 1 fimbrial expression, and thus as a regulator of the initial stages of biofilm development and, presumably, bacterial adherence to epithelial host cells also.

Distribution of fimH Gene in Local Isolates of Adhesive Uropathogenic Escherichia coli

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Omar Abdulkareem Ali ◽  
Ruqayah Qubtan Taha
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Uropathogenic Escherichia Coli ◽  
Adhesion Assay ◽  
E Coli ◽  
Type 1 Fimbriae ◽  
Formation Type ◽  
Uroepithelial Cells

Adhesion is an influential step for bacterial vigor in clinical micro-environments, type 1 fimbriae are essential virulence factors help uropathogenic E. coli in invasion and colonization of uroepithelial cells, the first step of UTIs and biofilm formation. Type 1 fimbriae of E. coli contain FimH protein at the tip encoding via fimH gene cluster, this study was conducted for determination the fimH gene distribution in uro-pathogenic E. coli isolated from UTIs patients. The results of adhesion assay show that (83.6%) of uropathogenic E. coli were high adherent isolates. While the results of E. coli fimH gene amplification prove that, of all E. coli isolates, the fimH gene was found in (87.1%), while among high adherent isolates it was found in (92.6%), and that Shows the function of type 1 fimbriae in the colonization and infection of urinary tracts in addition to other adhesions virulence agents of uropathogenic E. coli.

scholarly journals Uropathogenic Escherichia coli P and Type 1 Fimbriae Act in Synergy in a Living Host to Facilitate Renal Colonization Leading to Nephron Obstruction

2011 ◽  
Vol 7 (2) ◽  
pp. e1001298 ◽  
Author(s):  
Keira Melican ◽  
Ruben M. Sandoval ◽  
Abdul Kader ◽  
Lina Josefsson ◽  
George A. Tanner ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli ◽  
Type 1 Fimbriae

scholarly journals Type 1 Fimbriae, a Colonization Factor of Uropathogenic Escherichia coli, Are Controlled by the Metabolic Sensor CRP-cAMP

2009 ◽  
Vol 5 (2) ◽  
pp. e1000303 ◽  
Author(s):  
Claudia M. Müller ◽  
Anna Åberg ◽  
Jurate Straseviçiene ◽  
Levente Emődy ◽  
Bernt Eric Uhlin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli ◽  
Type 1 Fimbriae ◽  
Colonization Factor ◽  
Metabolic Sensor

scholarly journals In vitro adherence of type 1-fimbriated uropathogenic Escherichia coli to human ureteral mucosa.

1989 ◽  
Vol 57 (8) ◽  
pp. 2574-2579 ◽  
Author(s):  
K Fujita ◽  
T Yamamoto ◽  
T Yokota ◽  
R Kitagawa
Keyword(s):  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli

scholarly journals Antiadhesive properties of a quaternary structure-specific hybridoma antibody against type 1 fimbriae of Escherichia coli.

1983 ◽  
Vol 158 (4) ◽  
pp. 1114-1128 ◽  
Author(s):  
S N Abraham ◽  
D L Hasty ◽  
W A Simpson ◽  
E H Beachey
Keyword(s):  
Escherichia Coli ◽  
Monoclonal Antibodies ◽  
Biological Function ◽  
Quaternary Structure ◽  
Double Diffusion ◽  
Antigenic Determinants ◽  
Structural Determinants ◽  
Type 1 Fimbriae

The relationship between the structure and biological function of type 1 fimbriae of Escherichia coli was investigated using a set of monoclonal antibodies directed against conformation-specific antigenic determinants. Of three monoclonal antibodies tested, only one (clone CD3) prevented adhesion of the vaccine strain to epithelial cells or guinea pig erythrocytes. The antibody produced by CD3, but not that produced by the other two hybridoma clones (AA8 and GG1), precipitated isolated fimbriae by double diffusion in agar gel and was shown to bind in a highly discrete, periodic manner along the length of each of the fimbriae by immunoelectron microscopy. Immunoelectroblots of type 1 fimbrial subunits and polymers electrophoresed in SDS-gels indicated that the antibodies in AA8 and GG1 reacted only with fimbrial monomers (mol wt 17,000), whereas the antibody in CD3 reacted only with polymers of mol wt 102,000 (hexamers) or higher. ELISA inhibition assays demonstrated that dissociated fimbrial subunits lost their reactivity with antibody CD3 but gained reactivity with antibodies AA8 and GG1. Conversely, when allowed to reassemble in vitro in the presence of 5 mM MgCl2, the reassembled fimbriae lost their reactivity with antibodies AA8 and GG1 but regained reactivity with antibody CD3. These results demonstrated that certain antigenic epitopes are dependent on quaternary structural determinants, whereas others are independent of quaternary fimbrial structure and also are inaccessible for antibody binding in fimbriae once they have been assembled. These monoclonal antibodies should prove useful in studies of the structural determinants of the biological function of type 1 fimbriae as well as in studies of fimbrial synthesis, transport, and assembly.

Sign in / Sign up

Export Citation Format

Share Document