scholarly journals Yeast telomere maintenance is globally controlled by programmed ribosomal frameshifting and the nonsense-mediated mRNA decay pathway

Translation ◽  
2013 ◽  
Vol 1 (1) ◽  
pp. e24418 ◽  
Author(s):  
Vivek M. Advani ◽  
Ashton T. Belew ◽  
Jonathan D. Dinman
Keyword(s):  
Mrna Decay ◽  
Telomere Maintenance ◽  
Ribosomal Frameshifting ◽  
Nonsense Mediated Mrna Decay ◽  
Yeast Telomere

scholarly journals Detection and Degradation of Nonsense-mediated mRNA Decay Substrates Involve Two Distinct Upf1-bound Complexes

2018 ◽  
Author(s):  
Marine Dehecq ◽  
Laurence Decourty ◽  
Abdelkader Namane ◽  
Caroline Proux ◽  
Joanne Kanaan ◽  
...  
Keyword(s):  
Large Scale ◽  
Mrna Decay ◽  
Affinity Purification ◽  
Rna Degradation ◽  
Degradation Pathway ◽  
Telomere Maintenance ◽  
Mrna Decapping ◽  
Nonsense Mediated Mrna Decay ◽  
Decapping Enzyme

AbstractNonsense-mediated mRNA decay (NMD) is a translation-dependent RNA degradation pathway involved in many cellular pathways and crucial for telomere maintenance and embryo development. Core NMD factors Upf1, Upf2 and Upf3 are conserved from yeast to mammals, but a universal NMD model is lacking. We used affinity purification coupled with mass spectrometry and an improved data analysis protocol to obtain the first large-scale quantitative characterization of yeast NMD complexes in yeast (112 experiments). Unexpectedly, we identified two distinct complexes associated with Upf1: Detector (Upf1/2/3) and Effector. Effector contained the mRNA decapping enzyme, together with Nmd4 and Ebs1, two proteins that globally affected NMD and were critical for RNA degradation mediated by the Upf1 C-terminal helicase region. The fact that Nmd4 association to RNA was dependent on Detector components and the similarity between Nmd4/Ebs1 and mammalian Smg5-7 proteins suggest that in all eukaryotes NMD operates through successive Upf1-bound Detector and Effector complexes.

scholarly journals A Ribosomal Frameshifting Structure in the CCR5 mRNA Leads to Mirna-Stimulated Nonsense-Mediated mRNA Decay

2015 ◽  
Vol 108 (2) ◽  
pp. 238a
Author(s):  
Wojciech K. Kasprzak ◽  
Jonathan D. Dinman ◽  
Bruce A. Shapiro
Keyword(s):  
Mrna Decay ◽  
Ribosomal Frameshifting ◽  
Nonsense Mediated Mrna Decay
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