scholarly journals A tandem affinity purification tag of TGA2 for isolation of interacting proteins in Arabidopsis thaliana

2014 ◽  
Vol 9 (7) ◽  
pp. e29990
Author(s):  
Henrik U Stotz ◽  
Simone Findling ◽  
Ella Nukarinen ◽  
Wolfram Weckwerth ◽  
Martin J Mueller ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Affinity Purification ◽  
Tandem Affinity Purification ◽  
Interacting Proteins

scholarly journals A tandem affinity purification tag of TGA2 for isolation of interacting proteins inArabidopsis thaliana

2014 ◽  
Vol 9 (10) ◽  
pp. e972794 ◽  
Author(s):  
Henrik U Stotz ◽  
Simone Findling ◽  
Ella Nukarinen ◽  
Wolfram Weckwerth ◽  
Martin J Mueller ◽  
...  
Keyword(s):  
Affinity Purification ◽  
Tandem Affinity Purification ◽  
Interacting Proteins

Tandem Affinity Purification to Identify Cytosolic and Nuclear Gβγ-Interacting Proteins

2014 ◽  
pp. 161-184 ◽  
Author(s):  
Rhiannon Campden ◽  
Darlaine Pétrin ◽  
Mélanie Robitaille ◽  
Nicolas Audet ◽  
Sarah Gora ◽  
...  
Keyword(s):  
Affinity Purification ◽  
Tandem Affinity Purification ◽  
Interacting Proteins

scholarly journals Methyl-CpG-binding domain 9 (MBD9) is required for H2A.Z incorporation into chromatin at a subset of H2A.Z-enriched regions in the Arabidopsis genome

2018 ◽  
Author(s):  
Paja Sijacic ◽  
Dylan H. Holder ◽  
Marko Bajic ◽  
Roger B. Deal
Keyword(s):  
Arabidopsis Thaliana ◽  
Chromatin Remodeling ◽  
Potential Role ◽  
Affinity Purification ◽  
Binding Domain ◽  
Tandem Affinity Purification ◽  
Related Protein ◽  
Single Mutant ◽  
Chromatin Remodeling Complex ◽  
Associated Proteins

ABSTRACTThe SWR1 chromatin remodeling complex, which deposits the histone variant H2A.Z into nucleosomes, has been characterized in yeast and animals but had not been purified from plants. We used the conserved SWR1 subunit ACTIN RELATED PROTEIN 6 (ARP6) as bait in tandem affinity purification experiments to isolate associated proteins from Arabidopsis thaliana. We identified all 11 subunits found in yeast SWR1 and the homologous mammalian SRCAP complexes, demonstrating that this complex is conserved in plants. We also identified several additional proteins not previously associated with SWR1, including Methyl-CpG-BINDING DOMAIN 9 (MBD9). Since mbd9 mutant plants were phenotypically similar to arp6 mutants, we further explored a potential role for MBD9 in H2A.Z deposition. We found that MBD9 is required for proper H2A.Z incorporation at thousands of discrete sites, which represent a subset of the regions normally enriched with H2A.Z. Genetic analyses showed that arp6;mbd9 double mutants have far more severe phenotypes than either single mutant. In conjunction with the finding that MBD9 does not appear to be a core subunit of the Arabidopsis SWR1 complex, this suggests that MBD9 also has important roles beyond H2A.Z deposition. Our data establish the SWR1 complex as being conserved across eukaryotes and also provide new insights into the mechanisms that target H2A.Z to chromatin.

scholarly journals Tandem affinity purification in Drosophila: the advantages of the GS-TAP system

Fly ◽  
2008 ◽  
Vol 2 (4) ◽  
pp. 229-235 ◽  
Author(s):  
Phillip P. Kyriakakis ◽  
Marla Tipping ◽  
Louka Abed ◽  
Alexey Veraksa
Keyword(s):  
Affinity Purification ◽  
Tandem Affinity Purification

Tandem affinity purification of AtTERT reveals putative interaction partners of plant telomerase in vivo

PROTOPLASMA ◽  
2016 ◽  
Vol 254 (4) ◽  
pp. 1547-1562 ◽  
Author(s):  
Jana Majerská ◽  
Petra Procházková Schrumpfová ◽  
Ladislav Dokládal ◽  
Šárka Schořová ◽  
Karel Stejskal ◽  
...  
Keyword(s):  
Affinity Purification ◽  
Tandem Affinity Purification ◽  
Interaction Partners
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