scholarly journals The tissue-specific and developmentally regulated expression patterns of theSAUR41subfamily ofSMALL AUXIN UP RNAgenes

2013 ◽  
Vol 8 (8) ◽  
pp. e25283 ◽  
Author(s):  
Ting Qiu ◽  
Yong Chen ◽  
Miaomiao Li ◽  
Yingying Kong ◽  
Yubin Zhu ◽  
...  
2000 ◽  
Vol 275 (9) ◽  
pp. 6368-6374 ◽  
Author(s):  
Hitoshi Shirakawa ◽  
David Landsman ◽  
Yuri V. Postnikov ◽  
Michael Bustin

1986 ◽  
Vol 6 (7) ◽  
pp. 2624-2631 ◽  
Author(s):  
M Shani

A chimeric plasmid containing about 2/3 of the rat skeletal muscle actin gene plus 730 base pairs of its 5' flanking sequences fused to the 3' end of a human embryonic globin gene (D. Melloul, B. Aloni, J. Calvo, D. Yaffe, and U. Nudel, EMBO J. 3:983-990, 1984) was inserted into mice by microinjection into fertilized eggs. Eleven transgenic mice carrying the chimeric gene with or without plasmid pBR322 DNA sequences were identified. The majority of these mice transmitted the injected DNA to about 50% of their progeny. However, in transgenic mouse CV1, transmission to progeny was associated with amplification or deletion of the injected DNA sequences, while in transgenic mouse CV4 transmission was distorted, probably as a result of insertional mutagenesis. Tissue-specific expression was dependent on the removal of the vector DNA sequences from the chimeric gene sequences prior to microinjection. None of the transgenic mice carrying the chimeric gene together with plasmid pBR322 sequences expressed the introduced gene in striated muscles. In contrast, the six transgenic mice carrying the chimeric gene sequences alone expressed the inserted gene specifically in skeletal and cardiac muscles. Moreover, expression of the chimeric gene was not only tissue specific, but also developmentally regulated. Similar to the endogenous skeletal muscle actin gene, the chimeric gene was expressed at a relatively high level in cardiac muscle of neonatal mice and at a significantly lower level in adult cardiac muscle. These results indicate that the injected DNA included sufficient cis-acting control elements for its tissue-specific and developmentally regulated expression in transgenic mice.


2003 ◽  
Vol 133 (4) ◽  
pp. 1732-1746 ◽  
Author(s):  
Joseph A. Verica ◽  
Lee Chae ◽  
Hongyun Tong ◽  
Peter Ingmire ◽  
Zheng-Hui He

1993 ◽  
Vol 113 (2) ◽  
pp. 200-207 ◽  
Author(s):  
Tsuyoshi Tanabe ◽  
Mamoru Yamada ◽  
Takafumi Noma ◽  
Tadashi Kajii ◽  
Atsushi Nakazawa

1994 ◽  
Vol 269 (27) ◽  
pp. 18072-18075
Author(s):  
S. Hsu-Wong ◽  
S.D. Katchman ◽  
I. Ledo ◽  
M. Wu ◽  
J. Khillan ◽  
...  

1986 ◽  
Vol 6 (7) ◽  
pp. 2624-2631
Author(s):  
M Shani

A chimeric plasmid containing about 2/3 of the rat skeletal muscle actin gene plus 730 base pairs of its 5' flanking sequences fused to the 3' end of a human embryonic globin gene (D. Melloul, B. Aloni, J. Calvo, D. Yaffe, and U. Nudel, EMBO J. 3:983-990, 1984) was inserted into mice by microinjection into fertilized eggs. Eleven transgenic mice carrying the chimeric gene with or without plasmid pBR322 DNA sequences were identified. The majority of these mice transmitted the injected DNA to about 50% of their progeny. However, in transgenic mouse CV1, transmission to progeny was associated with amplification or deletion of the injected DNA sequences, while in transgenic mouse CV4 transmission was distorted, probably as a result of insertional mutagenesis. Tissue-specific expression was dependent on the removal of the vector DNA sequences from the chimeric gene sequences prior to microinjection. None of the transgenic mice carrying the chimeric gene together with plasmid pBR322 sequences expressed the introduced gene in striated muscles. In contrast, the six transgenic mice carrying the chimeric gene sequences alone expressed the inserted gene specifically in skeletal and cardiac muscles. Moreover, expression of the chimeric gene was not only tissue specific, but also developmentally regulated. Similar to the endogenous skeletal muscle actin gene, the chimeric gene was expressed at a relatively high level in cardiac muscle of neonatal mice and at a significantly lower level in adult cardiac muscle. These results indicate that the injected DNA included sufficient cis-acting control elements for its tissue-specific and developmentally regulated expression in transgenic mice.


2013 ◽  
Author(s):  
AL Bookout ◽  
Y Jeong ◽  
M Downes ◽  
RT Yu ◽  
RM Evans ◽  
...  

Zuriat ◽  
2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Nono Carsono ◽  
Christian Bachem

Tuberization in potato is a complex developmental process resulting in the differentiation of stolon into the storage organ, tuber. During tuberization, change in gene expression has been known to occur. To study gene expression during tuberization over the time, in vitro tuberization system provides a suitable tool, due to its synchronous in tuber formation. An early six days axillary bud growing on tuber induction medium is a crucial development since a large number of genes change in their expression patterns during this period. In order to identify, isolate and sequencing the genes which displaying differential pattern between tuberizing and non-tuberizing potato explants during six days in vitro tuberization, cDNA-AFLP fingerprint, method for the visualization of gene expression using cDNA as template which is amplified to generate an RNA-fingerprinting, was used in this experiment. Seventeen primer combinations were chosen based on their expression profile from cDNA-AFLP fingerprint. Forty five TDFs (transcript derived fragment), which displayed differential expressions, were obtained. Tuberizing explants had much more TDFs, which developmentally regulated, than those from non tuberizing explants. Seven TDFs were isolated, cloned and then sequenced. One TDF did not find similarity in the current databases. The nucleotide sequence of TDF F showed best similarity to invertase ezymes from the databases. The homology of six TDFs with known sequences is discussed in this paper.


PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e96007 ◽  
Author(s):  
Claes-Göran Reibring ◽  
Maha El Shahawy ◽  
Kristina Hallberg ◽  
Marie Kannius-Janson ◽  
Jeanette Nilsson ◽  
...  

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