scholarly journals Copper-induced intracellular calcium release requires extracellular calcium entry and activation of L-type voltage-dependent calcium channels in Ulva compressa

2012 ◽  
Vol 7 (7) ◽  
pp. 728-732 ◽  
Author(s):  
Alberto González ◽  
María de los Ángeles Cabrera ◽  
Macarena Mellado ◽  
Susana Cabello ◽  
Sebastián Márquez ◽  
...  
Keyword(s):  
Calcium Channels ◽  
Intracellular Calcium ◽  
Calcium Release ◽  
Calcium Entry ◽  
Extracellular Calcium ◽  
Ulva Compressa ◽  
Voltage Dependent ◽  
Voltage Dependent Calcium Channels ◽  
Intracellular Calcium Release

scholarly journals Copper-induced activation of TRPs and VDCCs triggers a calcium signature response regulating gene expression in Ectocarpus siliculosus

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4556 ◽  
Author(s):  
Alberto González ◽  
Claudio A. Sáez ◽  
Alejandra Moenne
Keyword(s):  
Calcium Channels ◽  
Intracellular Calcium ◽  
Protein Kinases ◽  
Calcium Release ◽  
Trp Channels ◽  
Calcium Entry ◽  
Extracellular Calcium ◽  
Green Macroalgae ◽  
Ectocarpus Siliculosus ◽  
Intracellular Calcium Release

In certain multicellular photoautotrophs, such as plants and green macroalgae, it has been demonstrated that calcium signaling importantly mediates tolerance to copper excess. However, there is no information in brown macroalgae, which are phylogenetically distant from green algae and plants. We have previously shown that chronic copper levels (2.5 μM) activate transient receptor potential (TRP) channels in the model brown macroalga Ectocarpus siliculosus, allowing extracellular calcium entry at 13, 29, 39 and 51 min. Here, we showed that intracellular calcium increases also occurred at 3 and 5 h of exposure; these increases were inhibited by antagonists of voltage-dependent calcium channels (VDCCs); a chelating agent of extracellular calcium; an antagonist of endoplasmic reticulum (ER) ATPase; and antagonists of cADPR-, NAADP- and IP3-dependent calcium channels. Thus, copper activates VDCCs allowing extracellular calcium entry and intracellular calcium release from the ER via cADPR-, IP3- and NAADP-dependent channels. Furthermore, the level of transcripts encoding a phytochelatin synthase (PS) and a metallothionein (MT) were analyzed in the alga exposed to 2.5 μM copper from 3 to 24 h. The level of ps and mt transcripts increased until 24 h and these increases were inhibited by antagonists of calmodulins (CaMs), calcineurin B-like proteins (CBLs) and calcium-dependent protein kinases (CDPKs). Finally, activation of VDCC was inhibited by a mixture of TRP antagonists and by inhibitors of protein kinases. Thus, copper-mediated activation of TRPs triggers VDCCs via protein kinases, allowing extracellular calcium entry and intracellular calcium release from ER that, in turn, activate CaMs, CBLs and CDPKs increasing expression of PS and MT encoding genes in E. siliculosus.

Calcium channels in PDGF-stimulated A172 cells open after intracellular calcium release and are not voltage-dependent

Cell Calcium ◽  
1991 ◽  
Vol 12 (7) ◽  
pp. 477-491 ◽  
Author(s):  
J. Szöllösi ◽  
B.G. Feuerstein ◽  
G. Vereb ◽  
H.A. Pershadsingh ◽  
L.J. Marton
Keyword(s):  
Calcium Channels ◽  
Intracellular Calcium ◽  
Calcium Release ◽  
Voltage Dependent ◽  
Intracellular Calcium Release

Protective Effect of Retinal Ischemia by Blockers of Voltage-dependent Calcium Channels and Intracellular Calcium Stores

2008 ◽  
Vol 28 (6) ◽  
pp. 847-856 ◽  
Author(s):  
Pindaro Dias Massote ◽  
Ana Cristina Nascimento Pinheiro ◽  
Cristina Guatimosim Fonseca ◽  
Marco Antonio Máximo Prado ◽  
André L. S. Guimarães ◽  
...  
Keyword(s):  
Calcium Channels ◽  
Protective Effect ◽  
Intracellular Calcium ◽  
Retinal Ischemia ◽  
Calcium Stores ◽  
Intracellular Calcium Stores ◽  
Voltage Dependent ◽  
Voltage Dependent Calcium Channels
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