scholarly journals ARF-GTPase as a Molecular Switch for Polar Auxin Transport Mediated by Vesicle Trafficking in Root Development

2007 ◽  
Vol 2 (2) ◽  
pp. 101-102 ◽  
Author(s):  
Kang Chong ◽  
Xiaolei Zhuang
Keyword(s):  
Root Development ◽  
Auxin Transport ◽  
Vesicle Trafficking ◽  
Molecular Switch ◽  
Polar Auxin Transport

scholarly journals B1L regulates lateral root development by exocytic vesicular trafficking-mediated polar auxin transport

2021 ◽  
Author(s):  
Yang Gang ◽  
Chen Bi-xia ◽  
Chen Tao ◽  
Chen Jia-hui ◽  
Sun Rui ◽  
...  
Keyword(s):  
Root Development ◽  
Lateral Root ◽  
Auxin Transport ◽  
Polar Auxin Transport ◽  
Vesicular Trafficking ◽  
Root Initiation ◽  
Root Primordia ◽  
Lateral Root Primordia ◽  
Lateral Root Development ◽  
Lateral Root Initiation

Auxin and auxin-mediated signaling pathways involved in the regulation of lateral root development are well documented. Although exocytic vesicle trafficking plays an important role in PIN-auxin-efflux carrier recycling, and polar auxin transport during lateral root formation, however, the mechanistic details of these processes are not well understood. Here, we demonstrate an essential regulatory mechanism of B1L that interacts with the exocyst to regulate PIN-mediated polar auxin transport and lateral root initiation. B1L is highly expressed in Arabidopsis roots, and genetic and cellular analyses have revealed that B1L is mainly involved in lateral root primordia initiation. Furthermore, DR5::GUS expression analyses revealed that auxin levels were higher in lateral root primordia of the b1l mutant than in the wild-type. Exogenous auxin treatment confirmed that the lateral root phenotype correlated closely with auxin levels. Additionally, auxin transport-inhibitory treatment indicated that B1L regulates auxin efflux. Consistently, b1l mutants exhibited higher levels of auxin efflux carriers PIN1-GFP and PIN3-GFP in lateral root primordia. Moreover, B1L interacts with the exocyst and functions in recycling PIN2-GFP. Finally, the b1l-1/exo70b1-1 double-mutant exhibited a significant increase in the number of lateral roots compared to the wildtype, b1l-1, and exo70b1-1. Collectively, this study improves our understanding of the highly sophisticated processes involved in exocytic vesicular trafficking-mediated polar auxin transport and lateral root initiation in plants.

Endophytic microbes Bacillus sp. LZR216-regulated root development is dependent on polar auxin transport in Arabidopsis seedlings

2015 ◽  
Vol 34 (6) ◽  
pp. 1075-1087 ◽  
Author(s):  
Jianfeng Wang ◽  
Yongqiang Zhang ◽  
Ying Li ◽  
Xiaomin Wang ◽  
Wenbin Nan ◽  
...  
Keyword(s):  
Root Development ◽  
Auxin Transport ◽  
Polar Auxin Transport ◽  
Bacillus Sp

scholarly journals Membrane Sterol Composition in Arabidopsis thaliana Affects Root Elongation via Auxin Biosynthesis

2021 ◽  
Vol 22 (1) ◽  
pp. 437
Author(s):  
Meng Wang ◽  
Panpan Li ◽  
Yao Ma ◽  
Xiang Nie ◽  
Markus Grebe ◽  
...  
Keyword(s):  
Root Elongation ◽  
Auxin Transport ◽  
Sterol Composition ◽  
Polar Auxin Transport ◽  
Sterol Biosynthesis ◽  
Auxin Biosynthesis ◽  
Root Tip ◽  
Auxin Signaling ◽  
Auxin Response ◽  
Short Root

Plant membrane sterol composition has been reported to affect growth and gravitropism via polar auxin transport and auxin signaling. However, as to whether sterols influence auxin biosynthesis has received little attention. Here, by using the sterol biosynthesis mutant cyclopropylsterol isomerase1-1 (cpi1-1) and sterol application, we reveal that cycloeucalenol, a CPI1 substrate, and sitosterol, an end-product of sterol biosynthesis, antagonistically affect auxin biosynthesis. The short root phenotype of cpi1-1 was associated with a markedly enhanced auxin response in the root tip. Both were neither suppressed by mutations in polar auxin transport (PAT) proteins nor by treatment with a PAT inhibitor and responded to an auxin signaling inhibitor. However, expression of several auxin biosynthesis genes TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS1 (TAA1) was upregulated in cpi1-1. Functionally, TAA1 mutation reduced the auxin response in cpi1-1 and partially rescued its short root phenotype. In support of this genetic evidence, application of cycloeucalenol upregulated expression of the auxin responsive reporter DR5:GUS (β-glucuronidase) and of several auxin biosynthesis genes, while sitosterol repressed their expression. Hence, our combined genetic, pharmacological, and sterol application studies reveal a hitherto unexplored sterol-dependent modulation of auxin biosynthesis during Arabidopsis root elongation.

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