scholarly journals Assessing functional role of three water deficit stress-induced genes in nonhost disease resistance using virus-induced gene silencing inNicotiana benthamiana

2010 ◽  
Vol 5 (5) ◽  
pp. 586-590 ◽  
Author(s):  
Muthappa Senthil-Kumar ◽  
Kirankumar S. Mysore
Keyword(s):  
Disease Resistance ◽  
Gene Silencing ◽  
Water Deficit ◽  
Functional Role ◽  
Water Deficit Stress ◽  
Virus Induced Gene Silencing ◽  
Induced Genes

Functional characterization of Nicotiana benthamiana homologs of peanut water deficit-induced genes by virus-induced gene silencing

Planta ◽  
2006 ◽  
Vol 225 (3) ◽  
pp. 523-539 ◽  
Author(s):  
M. Senthil-Kumar ◽  
Geetha Govind ◽  
Li Kang ◽  
Kirankumar S. Mysore ◽  
M. Udayakumar
Keyword(s):  
Gene Silencing ◽  
Water Deficit ◽  
Nicotiana Benthamiana ◽  
Functional Characterization ◽  
Virus Induced Gene Silencing ◽  
Induced Genes

Virus-induced gene silencing and its application in characterizing genes involved in water-deficit-stress tolerance

2008 ◽  
Vol 165 (13) ◽  
pp. 1404-1421 ◽  
Author(s):  
Muthappa Senthil-Kumar ◽  
H.V. Rame Gowda ◽  
Ramanna Hema ◽  
Kirankumar S. Mysore ◽  
Makarla Udayakumar
Keyword(s):  
Gene Silencing ◽  
Stress Tolerance ◽  
Water Deficit ◽  
Water Deficit Stress ◽  
Virus Induced Gene Silencing

Functional characterization of three water deficit stress-induced genes in tobacco and Arabidopsis: An approach based on gene down regulation

2010 ◽  
Vol 48 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Muthappa Senthil-Kumar ◽  
Ramanna Hema ◽  
Thumu Rao Suryachandra ◽  
H.V. Ramegowda ◽  
Ramaswamy Gopalakrishna ◽  
...  
Keyword(s):  
Water Deficit ◽  
Functional Characterization ◽  
Water Deficit Stress ◽  
Down Regulation ◽  
Induced Genes

scholarly journals Ubiquitin Extension Protein UEP1 Modulates Cell Death and Resistance to Various Pathogens in Tobacco

2019 ◽  
Vol 109 (7) ◽  
pp. 1257-1269
Author(s):  
You-Ping Xu ◽  
Yuan Zhao ◽  
Xiao-Yi Song ◽  
Yun-Feng Ye ◽  
Ren-Gang Wang ◽  
...  
Keyword(s):  
Cell Death ◽  
Disease Resistance ◽  
Ribosomal Protein ◽  
Mosaic Virus ◽  
Amine Oxidase ◽  
Active Species ◽  
26S Proteasome ◽  
Virus Induced Gene Silencing ◽  
Copper Amine Oxidase

Ubiquitin (Ub) extension proteins (UEPs) are fusion proteins of a Ub at the N terminus to a ribosomal protein. They are the main source of Ub and the only source of extension ribosomal protein. Although important roles of the Ub-26S proteasome system in various biological processes have been well established, direct evidence for the role of UEP genes in plant defense is rarely reported. In this study, we cloned a Ub-S27a-type UEP gene (NbUEP1) from Nicotiana benthamiana and demonstrated its function in cell death and disease resistance. Virus-induced gene silencing of NbUEP1 led to intensive cell death, culminating in whole-seedling withering. Transient RNA interference (RNAi) of NbUEP1 caused strong cell death in infiltrated areas, while stable NbUEP1-RNAi tobacco plants constitutively formed necrotic lesions in leaves. NbUEP1-RNAi plants exhibited increased resistance to the oomycete Pythium aphanidermatum and viruses Tobacco mosaic virus and Cucumber mosaic virus while displaying decreased resistance to the nematode Meloidogyne incognita compared with non-RNAi control plants. Transcription profiling analysis indicated that jasmonate and ethylene pathways, lipid metabolism, copper amine oxidase-mediated active species generation, glycine-rich proteins, vacuolar processing enzyme- and RD21-mediated cell death and defense regulation, and autophagy might be associated with NbUEP1-mediated cell death and resistance. Our results provided evidence for the important roles of plant UEPs in modulating plant cell death and disease resistance.

Role of silicon under water deficit stress in wheat: (Biochemical perspective): A review

2016 ◽  
Vol 37 (2) ◽  
Author(s):  
Sarang S. Sapre ◽  
Dinesh N. Vakharia
Keyword(s):  
Oxidative Stress ◽  
Water Deficit ◽  
Stress Factors ◽  
Water Deficit Stress ◽  
First Line ◽  
The Past ◽  
Rapid Absorption ◽  
Microscopic Studies ◽  
Stress Application

Silicon’s role in mediating resistance against various stresses has been a matter of focus in the past decade. Poaeceae family plants are known as high accumulators of silicon. Wheat shows rapid absorption, the optimum accumulation of silicon occurring at around 20 days. Silicon plays a role as a mechanical and a physiological barrier. It also alters the levels of osmolytes and antioxidant enzymes which are a first line of defense in the water deficit stress; also reducing the levels of oxidative stress factors such as hydrogen peroxide. But the results vary with respect to the modes of stress application and its duration. Nowadays, foliar mode of silicon application is carried out compared to the traditional soil application yielding some promising results. Further studies are needed to confirm the mechanisms governing protection which can be done with the comparison of the transcriptome analysis of the stressed plants and also microscopic studies revealing the site of deposition.

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