scholarly journals Short-term glucosamine infusion increases islet blood flow in anesthetized rats

Islets ◽  
2013 ◽  
Vol 5 (5) ◽  
pp. 201-206
Author(s):  
Xiang Gao ◽  
Leif Jansson ◽  
A Erik G Persson ◽  
Monica Sandberg
Keyword(s):  
Blood Flow ◽  
Short Term ◽  
Islet Blood Flow ◽  
Anesthetized Rats

Pancreatic islet blood flow in conscious rats during hyperglycemia and hypoglycemia

2001 ◽  
Vol 280 (6) ◽  
pp. R1601-R1605 ◽  
Author(s):  
M. Iwase ◽  
K. Tashiro ◽  
Y. Uchizono ◽  
D. Goto ◽  
M. Yoshinari
Keyword(s):  
Blood Flow ◽  
Pancreatic Islet ◽  
Organ Perfusion ◽  
Pancreatic Blood Flow ◽  
Pentobarbital Sodium ◽  
Time Points ◽  
Conscious Rats ◽  
Colored Microspheres ◽  
Islet Blood Flow ◽  
Anesthetized Rats

Anesthesia affects general hemodynamics and regulation of organ perfusion. We used colored microspheres to measure pancreatic islet blood flow in conscious rats at two time points, during either hyperglycemia or hypoglycemia. This method, using black and green microspheres, was validated by comparison with previous microsphere experiments and by lack of effect of a nonmetabolizable glucose analog, 3- O-methylglucose, on islet perfusion. Basal and glucose-stimulated islet blood flow levels were similar in pentobarbital sodium-anesthetized and conscious rats. However, the basal distribution of pancreatic blood flow was altered by anesthesia (fractional islet blood flow 5.8 ± 0.4% in conscious rats, 7.9 ± 0.8% in pentobarbital-anesthetized rats, P < 0.05). Insulin-induced hypoglycemia significantly increased whole pancreatic blood flow in conscious rats, whereas islet blood flow remained unchanged and fractional islet blood flow was decreased (5.8 ± 0.5% in the basal state, 4.2 ± 0.4% during hypoglycemia, P < 0.001). Methylatropine pretreatment significantly increased islet blood flow during hypoglycemia by 181%. This result suggests that prevention of hypoglycemia-induced increase in islet perfusion may be mediated, at least in part, by a cholinergic, vagal muscarinic mechanism.

scholarly journals KATP Channels and Pancreatic Islet Blood Flow in Anesthetized Rats: Increased Blood Flow Induced by Potassium Channel Openers

Diabetes ◽  
2003 ◽  
Vol 52 (8) ◽  
pp. 2043-2048 ◽  
Author(s):  
L. Jansson ◽  
M. Kullin ◽  
F. A. Karlsson ◽  
B. Bodin ◽  
J. B. Hansen ◽  
...  
Keyword(s):  
Blood Flow ◽  
Potassium Channel ◽  
Pancreatic Islet ◽  
Katp Channels ◽  
Potassium Channel Openers ◽  
Islet Blood Flow ◽  
Anesthetized Rats ◽  
Increased Blood Flow

Effect of exogenous cholecystokinin on islet blood flow in anesthetized rats

2003 ◽  
Vol 116 (1-3) ◽  
pp. 87-93 ◽  
Author(s):  
Masanori Iwase ◽  
Yuji Uchizono ◽  
Udai Nakamura ◽  
Sakae Nohara ◽  
Mitsuo Iida
Keyword(s):  
Blood Flow ◽  
Islet Blood Flow ◽  
Anesthetized Rats

Caerulein-induced pancreatitis and islet blood flow in anesthetized rats

2003 ◽  
Vol 113 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Magnus Johansson ◽  
Per-Ola Carlsson ◽  
Leif Jansson
Keyword(s):  
Blood Flow ◽  
Islet Blood Flow ◽  
Anesthetized Rats

Blood lipids affect rat islet blood flow regulation through β3-adrenoceptors

2014 ◽  
Vol 307 (8) ◽  
pp. E653-E663 ◽  
Author(s):  
Enyin Lai ◽  
Ulrika Pettersson ◽  
Alberto Delgado Verdugo ◽  
Per-Ola Carlsson ◽  
Birgitta Bodin ◽  
...  
Keyword(s):  
Blood Flow ◽  
Pancreatic Islet ◽  
Blood Lipids ◽  
Parasympathetic Nervous System ◽  
Serum Insulin ◽  
Insulin Response ◽  
Simultaneous Increase ◽  
Acute Administration ◽  
Islet Blood Flow ◽  
Anesthetized Rats

Pancreatic islet blood perfusion varies according to the needs for insulin secretion. We examined the effects of blood lipids on pancreatic islet blood flow in anesthetized rats. Acute administration of Intralipid to anesthetized rats increased both triglycerides and free fatty acids, associated with a simultaneous increase in total pancreatic and islet blood flow. A preceding abdominal vagotomy markedly potentiated this and led acutely to a 10-fold increase in islet blood flow associated with a similar increase in serum insulin concentrations. The islet blood flow and serum insulin response could be largely prevented by pretreatment with propranolol and the selective β3-adrenergic inhibitor SR-59230A. The nitric oxide synthase inhibitor NG-nitro-l-arginine methyl ester prevented the blood flow increase but was less effective in reducing serum insulin. Increased islet blood flow after Intralipid administration was also seen in islet and whole pancreas transplanted rats, i.e., models with different degrees of chronic islet denervation, but the effect was not as pronounced. In isolated vascularly perfused single islets Intralipid dilated islet arterioles, but this was not affected by SR-59230A. Both the sympathetic and parasympathetic nervous system are important for the coordination of islet blood flow and insulin release during hyperlipidemia, with a previously unknown role for β3-adrenoceptors.

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