scholarly journals The effects of oral carvacrol treatment against H2O2induced injury on isolated pancreas islet cells of rats

Islets ◽  
2013 ◽  
Vol 5 (4) ◽  
pp. 149-155 ◽  
Author(s):  
Asli San Dagli Gul ◽  
Ersin Fadillioglu ◽  
Ismail Karabulut ◽  
Ahmet Yesilyurt ◽  
Tuncay Delibasi
Keyword(s):  
Islet Cells ◽  
Pancreas Islet ◽  
Isolated Pancreas

scholarly journals IL-6 Promotes Islet β-Cell Dysfunction in Rat Collagen-Induced Arthritis

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Huan Jin ◽  
Yaogui Ning ◽  
Haotong Zhou ◽  
Youlian Wang
Keyword(s):  
Glucose Metabolism ◽  
Caspase 3 ◽  
Islet Cells ◽  
Type Ii Collagen ◽  
Intradermal Injection ◽  
Control Group ◽  
Collagen Induced Arthritis ◽  
Abnormal Glucose Metabolism ◽  
Pancreas Islet ◽  
Related Enzyme

The aim of this study was to explore the possible mechanism of rheumatoid arthritis- (RA-) related abnormal glucose metabolism. The model of collagen-induced arthritis (CIA) was established by intradermal injection of type II collagen into Wistar rats; complete Freund’s adjuvant injections were used as the control group. Fasting plasma glucose (FBG) was measured by the glucose oxidase method. Fasting insulin (FIns) and the expressions of IL-6 were detected by ELISA. Islet caspase-3 was examined by immunohistochemistry. On day 17 after immunization, FBG of the CIA group showed an elevated FBG value compared with the control group. Meanwhile, the FIns of group CIA was lower when compared with the control group. Interestingly, the inflammatory cytokine IL-6 expression was significantly increased when compared with the control group. As expected, the abnormal glucose metabolism was accompanied by the increased IL-6 expression. Furthermore, in line with the upregulated IL-6 expression, the apoptosis related enzyme caspase-3 was also markedly increased. These data showed that the elevated FBG in CIA may be associated with the reduced FIns level secondary to the overapoptosis of pancreas islet cells induced by IL-6.

Preganglionic innervation of the pancreas islet cells in the rat

1984 ◽  
Vol 10 (1) ◽  
pp. 27-42 ◽  
Author(s):  
P.G.M. Luiten ◽  
G.J. ter Horst ◽  
S.J. Koopmans ◽  
M. Rietberg ◽  
A.B. Steffens
Keyword(s):  
Islet Cells ◽  
Pancreas Islet

Occurance of Apoptosis during Ischemia in Porcine Pancreas Islet Cells

2003 ◽  
Vol 26 (3) ◽  
pp. 205-210 ◽  
Author(s):  
V. Stadlbauer ◽  
S. Schaffellner ◽  
F. Iberer ◽  
C. Lackner ◽  
B. Liegl ◽  
...  
Keyword(s):  
Islet Cells ◽  
Graft Loss ◽  
Warm Ischemia ◽  
Isolated Islets ◽  
Apoptotic Cells ◽  
Porcine Pancreas ◽  
Significant Difference ◽  
Pancreas Islet ◽  
Preservation Solutions ◽  
The Difference

Purpose Pancreas islet transplantation is a potential treatment of diabetes mellitus and porcine organs provide an easily available source of cells. Unfortunately quality and quantity of isolated islets are still not satisfactory. Apoptosis occurs in freshly isolated islets and plays a significant role in early graft loss. We evaluated the influence of four storage solutions on porcine pancreas islets. Method After warm ischemia of 15–20 minutes 12 organs were stored in 4 cold preservation solutions: Histidine-Tryptophan-Ketoglutarate solution (HTK), Hank's buffered saline solution (HBSS), University of Wisconsin (UW) solution and Ringer-Lactate (R). After cold ischemia for 100 minutes, organs were fixed in 3% formalin. Apoptotic cells were counted on hematocylin-eosin stainings. Results Most apoptotic cells were found in organs stored in R. Low numbers were found in the other groups. The difference between organs stored in R and organs stored in UW, HTK, or HBSS was highly significant. No significant difference could be found between UW, HTK and HBSS. Conclusion Cold and warm ischemia of the pancreas seems to induce apoptosis in islet cells. Preservation solutions cause less apoptosis than electrolyte solution. No significant differences could be found among the preservation solutions.

Silver Staining of Pancreatic Islets as Revealed by Electron Microscopy

1967 ◽  
Vol 25 ◽  
pp. 44-45
Author(s):  
Kazuaki Misugi ◽  
Nobuko Misugi ◽  
Hiroshi Yamada
Keyword(s):  
Pancreatic Islet ◽  
Silver Staining ◽  
Islet Cells ◽  
Severe Hypoglycemia ◽  
Granular Cell ◽  
Electron Microscopic Level ◽  
Staining Reaction ◽  
Electron Microscopic ◽  
Pancreatic Islet Cell ◽  
D Cell

The authors had described the fine structure of a type of pancreatic islet cell, which appeared different from typical alpha and beta cells, and tentatively considered that this third type of granular cell probably represents the D cell (Figure 1).Since silver staining has been widely used to differentiate different types of pancreatic islet cells by light microscopy, an attempt to examine this staining reaction at the electron microscopic level was made.Material and Method: Surgically removed specimens from three infants who suffered from severe hypoglycemia were used. The specimens were fixed and preserved in 20% neutral formalin. Frozen sections, 30 to 40 micron thick, were prepared and they were stained by Bielschowsky's method as modified by Suzuki (2). The stained sections were examined under a microscope and islet tissues were isolated. They were fixed in 1% osmium tetroxide in phosphate buffer for one hour and embedded in Epon 812 following dehydration through a series of alcohols and propylene oxide.

Iron status in diabetes mellitus

2019 ◽  
pp. 7-12
Keyword(s):  
Diabetes Mellitus ◽  
Beta Cells ◽  
Defense Mechanism ◽  
Acute Stress ◽  
Islet Cells ◽  
Iron Status ◽  
Acute Phase Reactant ◽  
Iron Deposition ◽  
Muscle Tissues ◽  
High Production

Diabetes mellitus can be defined as chronic metabolic disease which results from either relative or complete absence of insulin by the pancreatic beta islet cells. This in-turn may lead to hyperglycemia due to disturbances in the metabolism of glucose. In the human body, iron is con- sidered to be an effective pro-oxidant and participates in the generation of reactive oxygen species (ROS) such as hydroxyl radical. Because of the poor antioxidant defense mechanism of beta cells (low production of antioxidant enzymes such as catalase, glutathione peroxidase and dismutase), so they are highly prone to iron-induced oxidative stress and iron deposition in it and this will lead to apoptosis, and subsequently insulin deficiency. This iron deposition in beta cells will also lead to insulin resistance by reducing insulin extracting ability of the liver and inhibiting glucose uptake in muscle tissues and fats, this in turn will result in high production of hepatic glucose. Ferritin which is an acute phase reactant protein, that responds to acute stress like trauma, infections, tissue necrosis and surgery, it can produce diabetes mellitus either through inflammation or by increasing iron stores.

THERAPIE EINES METASTASIERENDEN INSELZELLTUMORS MIT STREPTOZOTOCIN

1971 ◽  
Vol 67 (2) ◽  
pp. 405-416 ◽  
Author(s):  
E. Nieschlag ◽  
H. Wombacher ◽  
F. J. Kroeger ◽  
L.V. Habighorst
Keyword(s):  
Liver Metastases ◽  
Blood Sugar ◽  
Mechanism Of Action ◽  
Islet Cell ◽  
Plasma Insulin ◽  
Severe Hypoglycaemia ◽  
Islet Cells ◽  
Intravenous Injections ◽  
High Affinity ◽  
Methyl Nitrosourea

A patient with a metastazing functional islet cell tumour suffering from severe hypoglycaemia was treated with streptozotocin. Four intravenous injections of 1.5 g streptozotocin each were administered in 4 to 6 days intervals. After the 4th injection there were no further episodes of hypoglycaemia, parenteral glucose administration could be stopped and blood sugar and plasma insulin, showing concentrations of up to 405 μU/ml before treatment, reached normal levels. The tumours in the pancreas disappeared and the liver metastases decreased in size and number as judged by arteriography. A hypothesis for the mechanism of action of streptozotocin is proposed. The glucose moiety is considered to facilitate a high affinity to the islet cells whereas the N-methyl-nitrosourea residue serves the active antitumour part of the molecule.

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