scholarly journals FGF-21 enhances islet engraftment in mouse syngeneic islet transplantation model

Islets ◽  
2010 ◽  
Vol 2 (4) ◽  
pp. 247-251 ◽  
Author(s):  
Taeko Uonaga ◽  
Kentaro Toyoda ◽  
Teru Okitsu ◽  
Xiaotong Zhuang ◽  
Shunsuke Yamane ◽  
...  
Keyword(s):  
Islet Transplantation ◽  
Syngeneic Islet Transplantation ◽  
Transplantation Model

scholarly journals XIAP inhibition of β-cell apoptosis reduces the number of islets required to restore euglycemia in a syngeneic islet transplantation model

Islets ◽  
2010 ◽  
Vol 2 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Annette Plesner ◽  
Galina Soukhatcheva ◽  
Robert G. Korneluk ◽  
C. Bruce Verchere
Keyword(s):  
Islet Transplantation ◽  
Cell Apoptosis ◽  
Β Cell ◽  
Syngeneic Islet Transplantation ◽  
Transplantation Model

scholarly journals Donor-Specific Regulatory T Cell-Mediated Immune Tolerance in an Intrahepatic Murine Allogeneic Islet Transplantation Model with Short-Term Anti-CD154 mAb Single Treatment

2020 ◽  
Vol 29 ◽  
pp. 096368972091387 ◽  
Author(s):  
Seok-Joo Lee ◽  
Hyun-Je Kim ◽  
Na-ri Byun ◽  
Chung-Gyu Park
Keyword(s):  
Islet Transplantation ◽  
Immune Tolerance ◽  
Treg Cells ◽  
Transplantation Tolerance ◽  
Single Treatment ◽  
Short Term ◽  
Donor Skin ◽  
First Time ◽  
Mixed Lymphocyte Reactions ◽  
Transplantation Model

Anti-CD154 blockade-based regimens remain unequaled in prolonging graft survival in various organ transplantation models. Several studies have focused on transplantation tolerance with the anti-CD154 blockade, but none of these studies has investigated the mechanisms associated with its use as the sole treatment in animal models, delaying our understanding of anti-CD154 blockade-mediated immune tolerance. The purpose of this study was to investigate the mechanism underlying the anti-CD154 monoclonal antibody (mAb) blockade in inducing immune tolerance using an intrahepatic murine allogeneic islet transplantation model. Allogeneic BALB/c AnHsd (BALB/c) islets were infused into the liver of diabetic C57BL/6 (B6) mice via the cecal vein. Anti-CD154 mAb (MR1) was administered on −1, 0, 1, 3, 5, and 7 d posttransplantation at 0.5 mg per mouse. We showed that short-term MR1 monotherapy could prolong the allogeneic islet grafts to more than 250 d in the murine intrahepatic islet transplantation model. The second islet grafts transplanted under the kidney capsule of the recipients were protected from rejection. We also found that rejection of same-donor skin grafts transplanted to the tolerant mice was modestly delayed. Using a DEREG mouse model, FoxP3+ regulatory T (Treg) cells were shown to play important roles in transplantation tolerance. In mixed lymphocyte reactions, Treg cells from the tolerant mice showed more potency in suppressing BALB/c splenocyte-stimulated Teff cell proliferation than those from naïve mice. In this study, we demonstrated for the first time that a short-term anti-CD154 mAb single treatment could induce FoxP3+ Treg cell-mediated immune tolerance in the intrahepatic murine allogeneic islet transplantation model.

Ducto-insular proliferation of beta-cells after syngeneic islet transplantation into the spleen of streptozotocin-diabetic lewis rats

1989 ◽  
Vol 5 (1) ◽  
pp. 77-83
Author(s):  
Frank Wohlrab ◽  
Siegfried Schmidt ◽  
Ingrid Klöting ◽  
Barbara Wilke ◽  
Lothar Cossel
Keyword(s):  
Beta Cells ◽  
Islet Transplantation ◽  
Lewis Rats ◽  
Syngeneic Islet Transplantation

scholarly journals Optimal Insulin Treatment in Syngeneic Islet Transplantation

2000 ◽  
Vol 9 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Juan F. Merino ◽  
Victor Nacher ◽  
Mercè Raurell ◽  
Montserrat Biarnés ◽  
Joan Soler ◽  
...  
Keyword(s):  
Islet Transplantation ◽  
Insulin Treatment ◽  
Syngeneic Islet Transplantation

scholarly journals Interleukin-1β and inducible form of nitric oxide synthase expression in early syngeneic islet transplantation

2007 ◽  
Vol 192 (1) ◽  
pp. 169-177 ◽  
Author(s):  
Marta Montolio ◽  
Montse Biarnés ◽  
Noèlia Téllez ◽  
Jessica Escoriza ◽  
Joan Soler ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Nitric Oxide Synthase ◽  
Islet Transplantation ◽  
Interleukin 1Β ◽  
Inos Mrna ◽  
No Production ◽  
Gene Expressions ◽  
Syngeneic Islet Transplantation ◽  
Oxide Synthase

Islets are particularly vulnerable in the initial days after transplantation when cell death results in the loss of more than half of the transplanted islet tissue. To determine whether a non-specific inflammation at the grafted site mediated by the local expression of inflammatory cytokines could play a role on the initial damage to transplanted islets, we studied the expressions of interleukin-1β (IL-1β) and inducible form of nitric oxide synthase (iNOS) after syngeneic islet transplantation. Insulin-treated streptozotocin-diabetic Lewis rats were syngeneically transplanted with 500 islets. Grafts were harvested 1, 3, or 7 days after transplantation, and the expressions of IL-1β and iNOS genes were determined by RT-PCR. IL-1β and iNOS mRNAs were detected in islets immediately after isolation, and were upregulated after transplantation. IL-1β mRNA was ninefold increased on day 1, was still sevenfold increased on day 3 after transplantation, and declined towards pretransplantation levels on day 7. iNOS mRNA showed a similar pattern of expression to that of IL-1β: on days 1 and 3 after transplantation it was 14-and 4-fold higher respectively than in freshly isolated islets. In addition, IL-1β and iNOS were identified in islet grafts and found to be produced mainly by CD68-positive macrophages. A low number of IL-1β- and iNOS-positive but CD68-negative cells were also identified suggesting that other cell types, in addition to macrophages, were involved in the expression of IL-1β and NO production in islet grafts. The finding of increased IL-1β and iNOS gene expressions in the initial days after islet transplantation and the presence of IL-β and iNOS proteins in the graft confirmed the presence of an early non-specific inflammatory response after islet transplantation. Overall, the data suggest that IL-1β plays a role in the extensive β-cell death found in the initial days after islet transplantation.

scholarly journals Subcutaneous Transplantation of Islets into Streptozocin-Induced Diabetic Rats

2005 ◽  
Vol 14 (8) ◽  
pp. 595-605 ◽  
Author(s):  
Craig R. Halberstadt ◽  
Deana Williams ◽  
Dwaine Emerich ◽  
Moses Goddard ◽  
Alfred V. Vasconcellos ◽  
...  
Keyword(s):  
Islet Transplantation ◽  
Cell Function ◽  
Diabetic Rats ◽  
Diabetic Patients ◽  
Pancreatic Islet Transplantation ◽  
Minimally Invasive Surgical Procedure ◽  
Minimally Invasive Surgical ◽  
Ease Of Access ◽  
Syngeneic Islet Transplantation ◽  
Type 1 Diabetic Patients

Pancreatic islet transplantation into type 1 diabetic patients is currently being performed by intraportal infusion. This method, albeit reproducible, has some disadvantages including potential development of portal hypertension, hemorrhage, and an inability to retrieve or detect the transplanted tissue. Other transplant sites have been examined in animal models including the omentum, peritoneal cavity, and the spleen. A transplant site that has not been successful in supporting functional islet tissue transplantation in humans is the subcutaneous space due primarily to the lack of a well-defined vascular bed. This site has many favorable characteristics such as ease of access for transplantation and potential for removal of the transplanted tissue with a minimally invasive surgical procedure. This report addresses the evaluation of a subcutaneously placed device for the support of rat syngeneic islet transplantation in a streptozocin-induced diabetic model. The data generated support the use of this device for islet engraftment. In addition, beta cell function in this device compared favorably with the function of islets transplanted to the renal subcapsular space as well as islets within the native pancreas.

Lack of Disease Recurrence in Diabetic BB/PFD Rats After Syngeneic Islet Transplantation

Autoimmunity ◽  
1993 ◽  
Vol 15 (2) ◽  
pp. 107-112 ◽  
Author(s):  
B. Kuttler ◽  
C. Mathieu ◽  
M. Waer ◽  
H. J. Hahn ◽  
R. Bouillon
Keyword(s):  
Islet Transplantation ◽  
Disease Recurrence ◽  
Syngeneic Islet Transplantation

scholarly journals Diabetes Is Reversed in a Murine Model by Marginal Mass Syngeneic Islet Transplantation Using a Subcutaneous Cell Pouch Device

2015 ◽  
Vol 99 (11) ◽  
pp. 2294-2300 ◽  
Author(s):  
Andrew R. Pepper ◽  
Rena Pawlick ◽  
Boris Gala-Lopez ◽  
Amanda MacGillivary ◽  
Delfina M. Mazzuca ◽  
...  
Keyword(s):  
Islet Transplantation ◽  
Murine Model ◽  
Syngeneic Islet Transplantation
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