scholarly journals Reduction of neutralization antibody against heterologous circulating strains in adults immunized with Japanese encephalitis live vaccine

2014 ◽  
Vol 10 (9) ◽  
pp. 2704-2705 ◽  
Author(s):  
Qing Ye ◽  
Xiao-Feng Li ◽  
Hui Zhao ◽  
Yong-Qiang Deng ◽  
Yan-Peng Xu ◽  
...  
Keyword(s):  
Japanese Encephalitis ◽  
Live Vaccine

scholarly journals Development and characterization of the replicon system of Japanese encephalitis live vaccine virus SA14-14-2

2013 ◽  
Vol 10 (1) ◽  
pp. 64 ◽  
Author(s):  
Shi-Hua Li ◽  
Xiao-Feng Li ◽  
Hui Zhao ◽  
Yong-Qiang Deng ◽  
Xue-Dong Yu ◽  
...  
Keyword(s):  
Japanese Encephalitis ◽  
Vaccine Virus ◽  
Live Vaccine

scholarly journals Molecular characterization of attenuated Japanese encephalitis live vaccine strain ML-17☆

Vaccine ◽  
2006 ◽  
Vol 24 (4) ◽  
pp. 402-411 ◽  
Author(s):  
Paresh Sumatilal Shah ◽  
Mariko Tanaka ◽  
Afjal Hossain Khan ◽  
Edward Gitau Matumbi Mathenge ◽  
Isao Fuke ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Vaccine Strain ◽  
Japanese Encephalitis ◽  
Live Vaccine ◽  
Live Vaccine Strain

scholarly journals In vitro and in vivo characterization of chimeric duck Tembusu virus based on Japanese encephalitis live vaccine strain SA14-14-2

2016 ◽  
Vol 97 (7) ◽  
pp. 1551-1556 ◽  
Author(s):  
Hong-Jiang Wang ◽  
Long Liu ◽  
Xiao-Feng Li ◽  
Qing Ye ◽  
Yong-Qiang Deng ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Japanese Encephalitis ◽  
Live Vaccine ◽  
Live Vaccine Strain ◽  
Duck Tembusu Virus ◽  
Tembusu Virus ◽  
In Vivo Characterization

scholarly journals A single nucleotide mutation in NS2A of Japanese encephalitis-live vaccine virus (SA14-14-2) ablates NS1’ formation and contributes to attenuation

2012 ◽  
Vol 93 (9) ◽  
pp. 1959-1964 ◽  
Author(s):  
Qing Ye ◽  
Xiao-Feng Li ◽  
Hui Zhao ◽  
Shi-Hua Li ◽  
Yong-Qiang Deng ◽  
...  
Keyword(s):  
Japanese Encephalitis ◽  
Structure Prediction ◽  
Secondary Structure Prediction ◽  
Vaccine Virus ◽  
Live Vaccine ◽  
Asia Pacific ◽  
Silent Mutation ◽  
Coding Region ◽  
Nucleotide Mutation

Japanese encephalitis (JE) remains the leading cause of viral encephalitis in the Asia-Pacific region, and the live vaccine SA14-14-2 is currently recommended by WHO and widely used in Asian countries with a good safety and efficacy profile. In this study, we demonstrated that SA14-14-2 failed to produce NS1’, the larger NS1-related protein, compared with its parental strain SA14 in various cells. Sequence analysis and secondary structure prediction identified a single silent mutation G66A in the NS2A-coding region of SA14-14-2 destabilized the conserved pseudoknot structure, which was associated with a −1 ribosomal frame shift event. Using reverse genetic technology and animal study, we provided solid evidence that this single silent mutation G66A in the NS2A gene abolished the production of NS1’ in vitro and reduced neurovirulence and neuroinvasiveness in mice. These findings provide critical information in understanding the molecular mechanism of JE vaccine attenuation and is critical for JE vaccine quality control.

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