scholarly journals Development and evaluation of a new immunohistochemistry-based test for the detection of rabies virus neutralizing antibodies

2014 ◽  
Vol 10 (5) ◽  
pp. 1359-1365 ◽  
Author(s):  
Shampur N Madhusudana ◽  
Bhavana V Malavalli ◽  
Ullas P Thankappan ◽  
Subha Sundramoorthy ◽  
Ashwin Y Belludi ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Neutralizing Antibodies

scholarly journals Serological response to rabies virus induced by commercial vaccines in cattle

2017 ◽  
Vol 47 (10) ◽  
Author(s):  
Mathias Martins ◽  
João Motta de Quadros ◽  
Eduardo Furtado Flores ◽  
Rudi Weiblen
Keyword(s):  
Rabies Virus ◽  
Neutralizing Antibodies ◽  
Vaccine Dose ◽  
Booster Vaccination ◽  
Serological Response ◽  
Serum Samples ◽  
Anamnestic Response ◽  
Post Vaccination ◽  
Antibody Titers ◽  
One Year

ABSTRACT: The antibody response to rabies virus (RABV) induced by commercial vaccines in heifers was investigated. For this, 84 heifers were vaccinated twice (30 days interval) with each of four vaccines (G1 = 14 animals; G2 = 24; G3 = 22 and G4 = 24) and received a booster vaccination 360 days later. Serum samples collected at different intervals after vaccination and 30 days after booster were submitted to a virus neutralizing (VN) assay for RABV antibodies. Thirty days after the second vaccine dose, 92% of the immunized animals presented VN titers ≥0.5UI/mL (geometric medium titers [GMT] 1.7 to 3.8UI/mL). At the day of the booster (360 days post-vaccination); however, the percentage of animals harboring antibody titers ≥0.5UI/mL had dropped to 31% (0-80% of the animals, depending on the vaccine), resulting in lower GMT (0.1 to 0.6UI/mL). Booster vaccination at day 360 resulted in a detectable anamnestic response in all groups, resulting in 83% of animals (65 to 100%) harboring VN titers ≥0.5UI/mL thirty days later (GMT 0.6 to 4.3UI/mL). These results indicated that these vaccines were able to induce an adequate anti-RABV response in all animals after prime vaccination (and after booster as well). However, the titers decreased, reaching titers <0.5UI/mL in approximately 70% of animals within the interval before the recommended booster. Thus, booster vaccination for rabies in cattle using the current vaccines should be performed before the recommended one-year interval, as to maintain neutralizing antibodies levels in most vaccinated animals.

scholarly journals INFLUENCE OF AGE FACTORS ON IMMUNIZABILITY OF MICE TO RABIES VIRUS

1940 ◽  
Vol 72 (4) ◽  
pp. 453-461 ◽  
Author(s):  
J. Casals
Keyword(s):  
Virus Infection ◽  
Rabies Virus ◽  
Neutralizing Antibodies ◽  
Age Factors ◽  
Swiss Mice ◽  
Virulent Virus ◽  
Rabies Virus Infection

1. W-Swiss mice 60 or more days old are more readily immunizable against rabies virus infection than 20 day old or younger mice; this difference in immunizability with increasing age is most conspicuous when vaccination with virulent virus is followed by intracerebral test infection and least apparent when vaccination with avirulent virus is followed by intramuscular test infection. 2. The titre of circulating neutralizing antibodies does not parallel the titre of immunity.

A new recombinant rabies virus expressing a green fluorescent protein: A novel and fast approach to quantify virus neutralizing antibodies

Biologicals ◽  
2019 ◽  
Vol 59 ◽  
pp. 56-61 ◽  
Author(s):  
Shuyun Qin ◽  
Dmitriy Volokhov ◽  
Elvira Rodionova ◽  
Christoph Wirblich ◽  
Matthias J. Schnell ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Rabies Virus ◽  
Neutralizing Antibodies ◽  
Fluorescent Protein ◽  
Protein A ◽  
Fast Approach ◽  
Green Fluorescent

scholarly journals Deficient Incorporation of Rabies Virus Glycoprotein into Virions Enhances Virus-Induced Immune Evasion and Viral Pathogenicity

Viruses ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 218 ◽  
Author(s):  
Chunfu Li ◽  
Hongliang Zhang ◽  
Lina Ji ◽  
Xiao Wang ◽  
Yongjun Wen ◽  
...  
Keyword(s):  
Immune Response ◽  
Rabies Virus ◽  
Recombinant Virus ◽  
Neutralizing Antibodies ◽  
Single Copy ◽  
Wild Type ◽  
Antiviral Immune Response ◽  
Glycoprotein G ◽  
Lethal Infection ◽  
Infected Cells

Previous studies have shown that wild-type (wt) rabies virus (RABV) evades the host immune response by restricting expression of glycoprotein (G), which blocks activation of dendritic cells (DCs) and induces production of virus-neutralizing antibodies (VNAs). In the present study, wt RABVs not only restricted G expression but also reduced incorporation of G into mature virions compared with laboratory-adapted viruses. A recombinant RABV expressing triple G was used to further determine whether G expression relates to incorporation. The recombinant virus showed higher expression and incorporation of G and activated more DCs than the virus that expressed a single copy of G. Removal of G from viruses using subtilisin or Dithiothreitol (DTT)/ Nonidet P-40 (NP40) almost completely abolishes DC activation and VNA production. Consequently, these G-depleted viruses cause lethal infection in mice. Thus, wt RABVs can subvert DC-induced antiviral immune response and maintain pathogenicity by decreasing G expression in infected cells and G incorporation into virions.

Revisiting rabies virus neutralizing antibodies through infecting BALB/c mice with live rabies virus

2018 ◽  
Vol 248 ◽  
pp. 39-43
Author(s):  
Yunlong Qin ◽  
Todd G. Smith ◽  
Felix Jackson ◽  
Nadia F. Gallardo-Romero ◽  
Clint N. Morgan ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Neutralizing Antibodies

scholarly journals Persistence of Rabies Virus-Neutralizing Antibodies after Vaccination of Rural Population following Vampire Bat Rabies Outbreak in Brazil

2016 ◽  
Vol 10 (9) ◽  
pp. e0004920 ◽  
Author(s):  
Rita Medeiros ◽  
Viviane Jusot ◽  
Guy Houillon ◽  
Anvar Rasuli ◽  
Luzia Martorelli ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Rural Population ◽  
Neutralizing Antibodies ◽  
Bat Rabies ◽  
Vampire Bat

scholarly journals Expression and Solubilization of Insect Cell-Based Rabies Virus Glycoprotein and Assessment of Its Immunogenicity and Protective Efficacy in Mice

2011 ◽  
Vol 18 (10) ◽  
pp. 1673-1679 ◽  
Author(s):  
R. Ramya ◽  
B. Mohana Subramanian ◽  
V. Sivakumar ◽  
R. L. Senthilkumar ◽  
K. R. S. Sambasiva Rao ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Insect Cell ◽  
Neutralizing Antibodies ◽  
Subunit Vaccine ◽  
Vaccine Development ◽  
Protective Efficacy ◽  
Vector System ◽  
Soluble Form ◽  
Rabies Virus Glycoprotein ◽  
Virus Glycoprotein

ABSTRACTRabies is a fatal zoonotic disease of serious public health and economic significance worldwide. The rabies virus glycoprotein (RVG) has been the major target for subunit vaccine development, since it harbors domains responsible for induction of virus-neutralizing antibodies, infectivity, and neurovirulence. The glycoprotein (G) was cloned using the baculovirus expression vector system (BEVS) and expressed inSpodoptera frugiperda(Sf-9) cells. In order to obtain a soluble form of G suitable for experimentation in mice, 18 different combinations of buffers and detergents were evaluated for their ability to solubilize the insect cell membrane-associated G. The combination that involved 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) detergent in lysis buffer 1, formulated with Tris, NaCl, 10% dimethyl sulfoxide (DMSO), and EDTA, gave the highest yield of soluble G, as evidenced by the experimental data. Subsequently, several other parameters, such as the concentration of CHAPS and the duration and temperature of the treatment for the effective solubilization of G, were optimized. The CHAPS detergent, buffered at a concentration of 0.4% to 0.7% (wt/vol) at room temperature (23 to 25°C) for 30 min to 1 h using buffer 1, containing 10% DMSO, resulted in consistently high yields. The G solubilized using CHAPS detergent was found to be immunogenic when tested in mice, as evidenced by high virus-neutralizing antibody titers in sera and 100% protection upon virulent intracerebral challenge with the challenge virus standard (CVS) strain of rabies virus. The results of the mice study indicated that G solubilized with CHAPS detergent retained the immunologically relevant domains in the native conformation, thereby paving the way for producing a cell-free and efficacious subunit vaccine.

scholarly journals Variability in Seroprevalence of Rabies Virus Neutralizing Antibodies and Associated Factors in a Colorado Population of Big Brown Bats (Eptesicus fuscus)

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e86261 ◽  
Author(s):  
Thomas J. O’Shea ◽  
Richard A. Bowen ◽  
Thomas R. Stanley ◽  
Vidya Shankar ◽  
Charles E. Rupprecht
Keyword(s):  
Rabies Virus ◽  
Neutralizing Antibodies ◽  
Associated Factors ◽  
Eptesicus Fuscus ◽  
Big Brown Bats

scholarly journals Calculating rabies virus neutralizing antibodies titres by flow cytometry

2002 ◽  
Vol 44 (3) ◽  
pp. 151-154 ◽  
Author(s):  
Juliano BORDIGNON ◽  
Fabiano COMIN ◽  
Sílvia Córdoba P. FERREIRA ◽  
Graciane M. M. CAPORALE ◽  
José Hermênio Cavalcante LIMA FILHO ◽  
...  
Keyword(s):  
Cell Culture ◽  
Flow Cytometry ◽  
Rabies Virus ◽  
Neutralizing Antibodies ◽  
Neutralizing Antibody ◽  
Standard Test ◽  
Serum Samples ◽  
Reference Serum ◽  
Infection Inhibition

The determination of the rabies neutralizing antibody (VNA) response after immunization against rabies is an acceptable index of the efficacy of a vaccine and a successful treatment. Several tests have been developed in attempt to improve the assessment of VNA, from mice inoculation to cell-culture fluorescence inhibition tests. All of them, however, present special difficulties in terms of reading or accuracy. The present study describes a neutralization test performed in cell-culture appraised by flow cytometry (FC). Serial dilutions of the serum samples were mixed in vitro with rabies virus before the addition of BHK-21 cells. After 24h-incubation, cells were released by trypsin treatment, fixed and permeabilized with a p-formaldehyde solution and stained with a rabies virus nucleocapsid protein-specific antibody conjugate. The percentage of virus infection inhibition caused by specific antibodies present in the serum were evaluated in a Beckton & Dickinson FACSCalibur® flow cytometer. A correlation curve between the IU/ml content and the percentage of infective inhibition was built with a reference serum and the VNA titers of serum samples were obtained by extrapolation. Titers obtained by FC and standard test showed an effective pairing results (p < 0.01), with a correlation coefficient (r) = 0.7. These results permit to envisage the FC as a suitable technique to evaluate VNA in sera from immunized animals and likely in human serum samples. Nevertheless, new studies comparing FC to gold-standard techniques are required for determining the FC values of Sensibility and Specificity .

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