scholarly journals Reconfiguring gene traps for new tasks using iTRAC

Fly ◽  
2011 ◽  
Vol 5 (4) ◽  
pp. 352-355 ◽  
Author(s):  
Zacharias Kontarakis ◽  
Nikolaos Konstantinides ◽  
Anastasios Pavlopoulos ◽  
Michalis Averof
Keyword(s):  
Gene Traps

[17] Retrovirus gene traps

1995 ◽  
pp. 263-275 ◽  
Author(s):  
Geoffrey G. Hicks ◽  
Er-Gang Shi ◽  
Jin Chen ◽  
Michael Roshon ◽  
Doug Williamson ◽  
...  
Keyword(s):  
Gene Traps

scholarly journals ROSA26Flpo deleter mice promote efficient inversion of conditional gene traps in vivo

genesis ◽  
2010 ◽  
Vol 48 (10) ◽  
pp. 603-606 ◽  
Author(s):  
Christopher S. Raymond ◽  
Philippe Soriano
Keyword(s):  
Gene Traps

scholarly journals Inducible Gene Trapping with Drug-Selectable Markers and Cre/loxP To Identify Developmentally Regulated Genes

2004 ◽  
Vol 24 (22) ◽  
pp. 9930-9941 ◽  
Author(s):  
You-Tzung Chen ◽  
Pentao Liu ◽  
Allan Bradley
Keyword(s):  
Retinoic Acid ◽  
Acid Treatment ◽  
Gene Trap ◽  
In Vitro Differentiation ◽  
Retinoic Acid Treatment ◽  
Developmentally Regulated ◽  
Gene Trapping ◽  
Gene Traps ◽  
Inducible Gene

ABSTRACT Gene trapping in mouse embryonic stem cells is an important genetic approach that allows simultaneous mutation of genes and generation of corresponding mutant mice. We designed a selection scheme with drug selection markers and Cre/loxP technology which allows screening of gene trap events that responded to a signaling molecule in a 96-well format. Nine hundred twenty gene trap clones were assayed, and 258 were classified as gene traps induced by in vitro differentiation. Sixty-five of the in vitro differentiation-inducible gene traps were also responsive to retinoic acid treatment. In vivo analysis revealed that 85% of the retinoic acid-inducible gene traps trapped developmentally regulated genes, consistent with the observation that genes induced by retinoic acid treatment are likely to be developmentally regulated. Our results demonstrate that the inducible gene trapping system described here can be used to enrich in vitro for traps in genes of interest. Furthermore, we demonstrate that the cre reporter is extremely sensitive and can be used to explore chromosomal regions that are not detectable with neo as a selection cassette.

scholarly journals Secretion Trap Tagging of Secreted and Membrane-Spanning Proteins Using Arabidopsis Gene Traps

2003 ◽  
Vol 132 (2) ◽  
pp. 698-708 ◽  
Author(s):  
Andrew T. Groover ◽  
Joseph R. Fontana ◽  
Juana M. Arroyo ◽  
Cristina Yordan ◽  
W. Richard McCombie ◽  
...  
Keyword(s):  
Arabidopsis Gene ◽  
Membrane Spanning ◽  
Gene Traps

scholarly journals CRISPR-Trap: a clean approach for the generation of gene knockouts and gene replacements in human cells

2018 ◽  
Vol 29 (2) ◽  
pp. 75-83 ◽  
Author(s):  
Stefan Reber ◽  
Jonas Mechtersheimer ◽  
Sofia Nasif ◽  
Julio Aguila Benitez ◽  
Martino Colombo ◽  
...  
Keyword(s):  
Human Cells ◽  
Dominant Negative ◽  
Open Reading Frame ◽  
Nonhomologous End Joining ◽  
End Joining ◽  
Reading Frame ◽  
First Intron ◽  
Gene Knockouts ◽  
Gene Traps

Conventional nonhomologous end joining–based gene knockouts can lead to the production of C-terminally truncated proteins with potentially residual or dominant negative functions. Combining CRISPR/Cas9 with gene traps targeting the first intron (CRISPR-Trap), however, completely prevents the expression of the open reading frame, resulting in clean gene knockouts.

scholarly journals Efficient conditional and promoter-specific in vivo expression of cDNAs of choice by taking advantage of recombinase-mediated cassette exchange using FlEx gene traps

2010 ◽  
Vol 38 (9) ◽  
pp. e106-e106 ◽  
Author(s):  
Laura Schebelle ◽  
Claudia Wolf ◽  
Carola Stribl ◽  
Tahereh Javaheri ◽  
Frank Schnütgen ◽  
...  
Keyword(s):  
Cassette Exchange ◽  
In Vivo Expression ◽  
Gene Traps

scholarly journals Gene Traps: Tools for Plant Development and Genomics

2000 ◽  
Vol 12 (7) ◽  
pp. 1007-1020 ◽  
Author(s):  
Patricia S. Springer
Keyword(s):  
Plant Development ◽  
Gene Traps

Gene Traps: Tools for Plant Development and Genomics

2000 ◽  
Vol 12 (7) ◽  
pp. 1007 ◽  
Author(s):  
Patricia S. Springer
Keyword(s):  
Plant Development ◽  
Gene Traps
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