scholarly journals Chromatin remodeling mediated by the FOXA1/A2 transcription factors activatesCFTRexpression in intestinal epithelial cells

Epigenetics ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. 557-565 ◽  
Author(s):  
Jenny L Kerschner ◽  
Nehal Gosalia ◽  
Shih-Hsing Leir ◽  
Ann Harris
Keyword(s):  
Transcription Factors ◽  
Epithelial Cells ◽  
Chromatin Remodeling ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial

scholarly journals Forkhead box transcription factors Foxa1 and Foxa2 are important regulators of Muc2 mucin expression in intestinal epithelial cells

2008 ◽  
Vol 369 (4) ◽  
pp. 1108-1113 ◽  
Author(s):  
Maria van der Sluis ◽  
Audrey Vincent ◽  
Janneke Bouma ◽  
Anita Korteland-Van Male ◽  
Johannes B. van Goudoever ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Forkhead Box ◽  
Mucin Expression

Sp1 and Sp3 mediate NHE2 gene transcription in the intestinal epithelial cells

2007 ◽  
Vol 293 (1) ◽  
pp. G146-G153 ◽  
Author(s):  
Ping Hua ◽  
Hua Xu ◽  
Jennifer K. Uno ◽  
Maciej A. Lipko ◽  
Jiali Dong ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Epithelial Cells ◽  
Gene Transcription ◽  
Promoter Region ◽  
Promoter Activity ◽  
Intestinal Epithelial Cells ◽  
Gene Promoter ◽  
Minimal Promoter ◽  
Intestinal Epithelial ◽  
Minimal Promoter Region

Our previous studies have identified a minimal Sp1-driven promoter region (nt −36/+116) directing NHE2 expression in mouse renal epithelial cells. However, this minimal promoter region was not sufficient to support active transcription of NHE2 gene in the intestinal epithelial cells, suggesting the need for additional upstream regulatory elements. In the present study, we used nontransformed rat intestinal epithelial (RIE) cells as a model to identify the minimal promoter region and transcription factors necessary for the basal transcription of rat NHE2 gene in the intestinal epithelial cells. We identified a region within the rat NHE2 gene promoter located within nt −67/−43 upstream of transcription initiation site as indispensable for the promoter function in intestinal epithelial cells. Mutations at nt −56/−51 not only abolished the DNA-protein interaction in this region, but also completely abolished NHE2 gene promoter activity in RIE cells. Supershift assays revealed that Sp1 and Sp3 interact with this promoter region, but, contrary to the minimal promoter indispensable for renal expression of NHE2, both transcription factors expressed individually in Drosophila SL2 cells activated rat NHE2 gene promoter. Moreover, Sp1 was a weaker transactivator and when coexpressed in SL2 cells it reduced Sp3-mediated NHE2 basal promoter activity. Furthermore, DNase I footprinting confirmed that nt −58/−51 is protected by nuclear protein from RIE cells. We conclude that the mechanism of basal control of rat NHE2 gene promoter activity is different in the renal and intestinal epithelium, with Sp3 being the major transcriptional activator of NHE2 gene transcription in the intestinal epithelial cells.

Loading of transmural pressure stimulates interleukin-6 synthesis in intestinal epithelial cells: Activation of transcription factors

2000 ◽  
Vol 118 (4) ◽  
pp. A1351
Author(s):  
Hiroshi Kishikawa ◽  
Soichiro Miura ◽  
Hiromasa Nakamizo ◽  
Hideo Yoshida ◽  
Masahiko Hirokawa ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Epithelial Cells ◽  
Interleukin 6 ◽  
Intestinal Epithelial Cells ◽  
Transmural Pressure ◽  
Intestinal Epithelial ◽  
Activation Of Transcription

Three-dimensional organization and functional relationships of endocytotic compartments in pig intestinal epithelial cells

1992 ◽  
Vol 50 (1) ◽  
pp. 576-577
Author(s):  
Julian P. Heath ◽  
Buford L. Nichols ◽  
László G. Kömüves
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Three Dimensional ◽  
Intestinal Epithelial ◽  
Cell Surfaces ◽  
Basal Surface ◽  
Functional Relationships

The newborn pig intestine is adapted for the rapid and efficient absorption of nutrients from colostrum. In enterocytes, colostral proteins are taken up into an apical endocytotic complex of channels that transports them to target organelles or to the basal surface for release into the circulation. The apical endocytotic complex of tubules and vesicles clearly is a major intersection in the routes taken by vesicles trafficking to and from the Golgi, lysosomes, and the apical and basolateral cell surfaces.Jejunal tissues were taken from piglets suckled for up to 6 hours and prepared for electron microscopy and immunocytochemistry as previously described.

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