scholarly journals High quality methylome-wide investigations through next-generation sequencing of DNA from a single archived dry blood spot

Epigenetics ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. 542-547 ◽  
Author(s):  
Karolina A. Aberg ◽  
Lin Y. Xie ◽  
Srilaxmi Nerella ◽  
William E. Copeland ◽  
E. Jane Costello ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
High Quality ◽  
Generation Sequencing ◽  
Blood Spot

Archived neonatal dried blood spot samples can be used for accurate whole genome and exome-targeted next-generation sequencing

2013 ◽  
Vol 110 (1-2) ◽  
pp. 65-72 ◽  
Author(s):  
Mads Vilhelm Hollegaard ◽  
Jonas Grauholm ◽  
Ronni Nielsen ◽  
Jakob Grove ◽  
Susanne Mandrup ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Dried Blood Spot ◽  
Whole Genome ◽  
Next Generation ◽  
Targeted Next Generation Sequencing ◽  
Generation Sequencing ◽  
Blood Spot

scholarly journals Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics

Cancers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1691
Author(s):  
Muscarella ◽  
Fabrizio ◽  
De Bonis ◽  
Mancini ◽  
Balsamo ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Scale Up ◽  
Cancer Diagnostics ◽  
Sequencing Analysis ◽  
Library Preparation ◽  
Next Generation ◽  
High Quality ◽  
High Coverage ◽  
Dna Recovery ◽  
Generation Sequencing

Thanks to personalized medicine trends and collaborations between industry, clinical research groups and regulatory agencies, next generation sequencing (NGS) is turning into a common practice faster than one could have originally expected. When considering clinical applications of NGS in oncology, a rapid workflow for DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue samples, as well as producing high quality library preparation, can be real challenges. Here we consider these targets and how applying effective automation technology to NGS workflows may help improve yield, timing and quality-control. We firstly evaluated DNA recovery from archived FFPE blocks from three different manual extraction methods and two automated extraction workstations. The workflow was then implemented to somatic (lung/colon panel) and germline (BRCA1/2) library preparation for NGS analysis exploiting two automated workstations. All commercial kits gave good results in terms of DNA yield and quality. On the other hand, the automated workstation workflow has been proven to be a valid automatic extraction system to obtain high quality DNA suitable for NGS analysis (lung/colon Ampli-seq panel). Moreover, it can be efficiently integrated with an open liquid handling platform to provide high-quality libraries from germline DNA with more reproducibility and high coverage for targeted sequences in less time (BRCA1/2). The introduction of automation in routine workflow leads to an improvement of NGS standardization and increased scale up of sample preparations, reducing labor and timing, with optimization of reagents and management.

scholarly journals Small lung tumor biopsy samples are feasible for high quality targeted next generation sequencing

2019 ◽  
Vol 110 (8) ◽  
pp. 2652-2657 ◽  
Author(s):  
Hidenori Kage ◽  
Shinji Kohsaka ◽  
Aya Shinozaki‐Ushiku ◽  
Yoshihisa Hiraishi ◽  
Jiro Sato ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Lung Tumor ◽  
Next Generation ◽  
High Quality ◽  
Tumor Biopsy ◽  
Targeted Next Generation Sequencing ◽  
Generation Sequencing

High-quality RNA extraction from copepods for Next Generation Sequencing: A comparative study

2015 ◽  
Vol 24 ◽  
pp. 115-118 ◽  
Author(s):  
Sneha Asai ◽  
Adrianna Ianora ◽  
Chiara Lauritano ◽  
Penelope K. Lindeque ◽  
Ylenia Carotenuto
Keyword(s):  
Next Generation Sequencing ◽  
Comparative Study ◽  
Rna Extraction ◽  
Next Generation ◽  
High Quality ◽  
Generation Sequencing
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