scholarly journals Drugging the heat shock factor 1 pathway: Exploitation of the critical cancer cell dependence on the guardian of the proteome

Cell Cycle ◽  
2009 ◽  
Vol 8 (23) ◽  
pp. 3806-3808 ◽  
Author(s):  
Emmanuel de Billy ◽  
Marissa V. Powers ◽  
Jennifer R. Smith ◽  
Paul Workman
Keyword(s):  
Heat Shock ◽  
Cancer Cell ◽  
Heat Shock Factor ◽  
Heat Shock Factor 1 ◽  
The Guardian

scholarly journals Upregulation of lactate dehydrogenase A by ErbB2 through heat shock factor 1 promotes breast cancer cell glycolysis and growth

Oncogene ◽  
2009 ◽  
Vol 28 (42) ◽  
pp. 3689-3701 ◽  
Author(s):  
Y H Zhao ◽  
M Zhou ◽  
H Liu ◽  
Y Ding ◽  
H T Khong ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lactate Dehydrogenase ◽  
Heat Shock ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Heat Shock Factor ◽  
Heat Shock Factor 1

Heat-shock factor 1 both positively and negatively affects cellular clonogenic growth depending on p53 status

2013 ◽  
Vol 452 (2) ◽  
pp. 321-329 ◽  
Author(s):  
Chau H. Nguyen ◽  
Benjamin J. Lang ◽  
Ryan C. C. Chai ◽  
Jessica L. Vieusseux ◽  
Michelle M. Kouspou ◽  
...  
Keyword(s):  
Heat Shock ◽  
Cancer Cell ◽  
Heat Shock Factor ◽  
Mutant P53 ◽  
Heat Shock Factor 1 ◽  
Dependent Manner ◽  
Wild Type ◽  
Clonogenic Growth ◽  
Wild Type P53

HSF1 (heat-shock factor 1) is the master regulator of the heat-shock response; however, it is also activated by cancer-associated stresses and supports cellular transformation and cancer progression. We examined the role of HSF1 in relation to cancer cell clonogenicity, an important attribute of cancer cells. Ectopic expression or HSF1 knockdown demonstrated that HSF1 positively regulated cancer cell clonogenic growth. Furthermore, knockdown of mutant p53 indicated that HSF1 actions were mediated via a mutant p53-dependent mechanism. To examine this relationship more specifically, we ectopically co-expressed mutant p53R273H and HSF1 in the human mammary epithelial cell line MCF10A. Surprisingly, within this cellular context, HSF1 inhibited clonogenicity. However, upon specific knockdown of endogenous wild-type p53, leaving mutant p53R273H expression intact, HSF1 was observed to greatly enhance clonogenic growth of the cells, indicating that HSF1 suppressed clonogenicity via wild-type p53. To confirm this we ectopically expressed HSF1 in non-transformed and H-RasV12-transformed MCF10A cells. As expected, HSF1 significantly reduced clonogenicity, altering wild-type p53 target gene expression levels consistent with a role of HSF1 increasing wild-type p53 activity. In support of this finding, knockdown of wild-type p53 negated the inhibitory effects of HSF1 expression. We thus show that HSF1 can affect clonogenic growth in a p53 context-dependent manner, and can act via both mutant and wild-type p53 to bring about divergent effects upon clonogenicity. These findings have important implications for our understanding of HSF1's divergent roles in cancer cell growth and survival as well as its disparate effect on mutant and wild-type p53.

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