scholarly journals Effects of cell number on teratoma formation by human embryonic stem cells

Cell Cycle ◽  
2009 ◽  
Vol 8 (16) ◽  
pp. 2608-2612 ◽  
Author(s):  
Andrew S. Lee ◽  
Chad Tang ◽  
Feng Cao ◽  
Xiaoyan Xie ◽  
Koen van der Bogt ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Number ◽  
Teratoma Formation

scholarly journals A Scalable Approach to Prevent Teratoma Formation of Human Embryonic Stem Cells

2012 ◽  
Vol 287 (39) ◽  
pp. 32338-32345 ◽  
Author(s):  
Zhili Rong ◽  
Xuemei Fu ◽  
Meiyan Wang ◽  
Yang Xu
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Teratoma Formation

The anti-apoptotic gene survivin contributes to teratoma formation by human embryonic stem cells

2009 ◽  
Vol 27 (3) ◽  
pp. 281-287 ◽  
Author(s):  
Barak Blum ◽  
Ori Bar-Nur ◽  
Tamar Golan-Lev ◽  
Nissim Benvenisty
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Apoptotic Gene ◽  
Teratoma Formation

scholarly journals Teratoma formation by human embryonic stem cells: Evaluation of essential parameters for future safety studies

2009 ◽  
Vol 2 (3) ◽  
pp. 198-210 ◽  
Author(s):  
Hannes Hentze ◽  
Poh Loong Soong ◽  
Siew Tein Wang ◽  
Blaine W. Phillips ◽  
Thomas C. Putti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Safety Studies ◽  
Teratoma Formation

scholarly journals StemCell Keep™ is Effective for Cryopreservation of Human Embryonic Stem Cells by Vitrification

2017 ◽  
Vol 26 (5) ◽  
pp. 773-787 ◽  
Author(s):  
Akemi Ota ◽  
Kazuaki Matsumura ◽  
Jun-Jae Lee ◽  
Shoichiro Sumi ◽  
Soung-Hyu Hyon
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Number ◽  
Mouse Fibroblast ◽  
Stem Cell Markers ◽  
Rt Pcr ◽  
Induced Pluripotent ◽  
Attachment Ability

Safe and stable cryopreservation is critical for research involving human embryonic stem cells (hESCs). Dimethyl sulfoxide (DMSO) is a popular cryoprotective agent; however, its cytotoxicity cannot be ignored. Thus, there is a need for an alternate cryoprotectant. We reported previously that a novel cryopreservation reagent, StemCell Keep™ (SCK), was effective for cryopreserving human induced pluripotent stem cells (hiPSCs) by vitrification. Because hESCs and hiPSCs are not identical, the current study examined the use of SCK on hESCs. hESCs cryopreserved with SCK were thawed and cultured on SNL 76/7 cells, which were derived from a mouse fibroblast STO cell line transformed with neomycin resistance and murine LIF genes. After cryopreservation, cultured hESCs were assessed for their attachment ability and characterized by alkaline phosphatase (AP) and immunocytochemical (ICC) staining, fluorescence-activated cell sorting (FACS), reverse transcription polymerase chain reaction (RT-PCR), and karyotyping. The proliferation of SCK-cryopreserved hESCs cultured on SNL cells, or in feeder-free conditions, was higher than that of cells preserved in a solution of 2 M DMSO, 1 M acetamide, and 3 M propylene glycol (DAP). The cell number with SCK-cryopreserved hESCs was about twice that of hESCs cryopreserved in DAP. The pluripotency of SCK-cryopreserved hESCs was similar to that of DAP-cryopreserved hESCs based on AP staining. Data from ICC, FACS, and RT-PCR analyses showed that stem cell markers were continually expressed on SCK-cryopreserved hESCs. The teratoma assay showed that SCK-cryopreserved hESCs differentiated into three germ layers. Furthermore, SCK-cryopreserved hESCs had normal karyotypes. These data indicate that SCK was effective for cryopreservation of hESCs by vitrification.

scholarly journals Teratoma formation of human embryonic stem cells in three-dimensional perfusion culture bioreactors

2012 ◽  
Vol 7 (9) ◽  
pp. 729-741 ◽  
Author(s):  
H. Stachelscheid ◽  
A. Wulf-Goldenberg ◽  
K. Eckert ◽  
J. Jensen ◽  
J. Edsbagge ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Perfusion Culture ◽  
Teratoma Formation

Teratoma Formation by Human Embryonic Stem Cells Is Site Dependent and Enhanced by the Presence of Matrigel

2009 ◽  
Vol 18 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Tatyana A. Prokhorova ◽  
Linda M. Harkness ◽  
Ulrik Frandsen ◽  
Nicholas Ditzel ◽  
Henrik D. Schrøder ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Teratoma Formation

scholarly journals Teratoma Formation Assays with Human Embryonic Stem Cells: A Rationale for One Type of Human-Animal Chimera

2007 ◽  
Vol 1 (3) ◽  
pp. 253-258 ◽  
Author(s):  
M. William Lensch ◽  
Thorsten M. Schlaeger ◽  
Leonard I. Zon ◽  
George Q. Daley
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Human Animal ◽  
Teratoma Formation
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