scholarly journals Nucleosome binding proteins potentiate ATM activation and DNA damage response by modifying chromatin

Cell Cycle ◽  
2009 ◽  
Vol 8 (11) ◽  
pp. 1641-1644 ◽  
Author(s):  
Gabi Gerlitz ◽  
Michael Bustin
Keyword(s):  
Dna Damage ◽  
Dna Damage Response ◽  
Binding Proteins ◽  
Damage Response ◽  
Nucleosome Binding

scholarly journals PARprolink: a photoaffinity probe for identifying poly(ADP-ribose)-binding proteins

2020 ◽  
Author(s):  
Morgan Dasovich ◽  
Morgan Q. Beckett ◽  
Scott Bailey ◽  
Shao-En Ong ◽  
Marc M. Greenberg ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Damage Response ◽  
Rna Processing ◽  
Binding Proteins ◽  
Post Translational Modification ◽  
Mobility Shift ◽  
Binding Partners ◽  
Damage Response ◽  
Prostate Cancers ◽  
Electrophoretic Mobility Shift Assays

ABSTRACTPost-translational modification of proteins with poly(ADP-ribose) (PAR) is an important component of the DNA damage response. Four PAR synthesis inhibitors have recently been approved for the treatment of breast, ovarian, and prostate cancers. Despite its clinical significance, a molecular understanding of PAR function, including its binding partners, remains incomplete. In this work, we synthesize a PAR photoaffinity probe that captures and isolates endogenous PAR binders. Our method identified dozens of known PAR-binding proteins and hundreds of novel binders involved in DNA repair, RNA processing, and metabolism. PAR binding by eight candidates was confirmed using pull-down and/or electrophoretic mobility shift assays. Using PAR probes of defined lengths, we detected proteins that preferentially bind to 40-mer over 8-mer PAR, indicating that polymer length may regulate the outcome and timing of PAR signaling pathways. This investigation produces the first census of PAR-binding proteins, provides a proteome-wide view of length-selective PAR binding, and associates PAR binding with RNA metabolism and the formation of biomolecular condensates.

scholarly journals CRN13 candidate effectors from plant and animal eukaryotic pathogens are DNA-binding proteins which trigger host DNA damage response

2015 ◽  
Vol 210 (2) ◽  
pp. 602-617 ◽  
Author(s):  
Diana Ramirez-Garcés ◽  
Laurent Camborde ◽  
Michiel J. C. Pel ◽  
Alain Jauneau ◽  
Yves Martinez ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Binding ◽  
Dna Damage Response ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Damage Response
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