scholarly journals Nitrogen Oxide-Releasing Aspirin Induces Histone H2AX Phosphorylation, ATM Activation and Apoptosis Preferentially in S-Phase Cells: Involvement of Reactive Oxygen Species

Cell Cycle ◽  
2006 ◽  
Vol 5 (15) ◽  
pp. 1669-1674 ◽  
Author(s):  
Toshiki Tanaka ◽  
Akira Kurose ◽  
H. Dorota Halicka ◽  
Xuan Huang ◽  
Frank Traganos ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Nitrogen Oxide ◽  
Reactive Oxygen ◽  
S Phase ◽  
Oxygen Species ◽  
H2ax Phosphorylation ◽  
Histone H2ax ◽  
Histone H2ax Phosphorylation

scholarly journals Regulation of Late G1/S Phase Transition and APCCdh1 by Reactive Oxygen Species

2006 ◽  
Vol 26 (12) ◽  
pp. 4701-4711 ◽  
Author(s):  
Courtney G. Havens ◽  
Alan Ho ◽  
Naohisa Yoshioka ◽  
Steven F. Dowdy
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Cycle ◽  
Cell Size ◽  
Cell Cycle Progression ◽  
Reactive Oxygen ◽  
Cyclin A ◽  
S Phase ◽  
Oxygen Species ◽  
Cycle Progression ◽  
Mitochondrial Mass

ABSTRACT Proliferating cells have a higher metabolic rate than quiescent cells. To investigate the role of metabolism in cell cycle progression, we examined cell size, mitochondrial mass, and reactive oxygen species (ROS) levels in highly synchronized cell populations progressing from early G1 to S phase. We found that ROS steadily increased, compared to cell size and mitochondrial mass, through the cell cycle. Since ROS has been shown to influence cell proliferation and transformation, we hypothesized that ROS could contribute to cell cycle progression. Antioxidant treatment of cells induced a late-G1-phase cell cycle arrest characterized by continued cellular growth, active cyclin D-Cdk4/6 and active cyclin E-Cdk2 kinases, and inactive hyperphosphorylated pRb. However, antioxidant-treated cells failed to accumulate cyclin A protein, a requisite step for initiation of DNA synthesis. Further examination revealed that cyclin A continued to be ubiquitinated by the anaphase promoting complex (APC) and to be degraded by the proteasome. This antioxidant arrest could be rescued by overexpression of Emi1, an APC inhibitor. These observations reveal an intrinsic late-G1-phase checkpoint, after transition across the growth factor-dependent G1 restriction point, that links increased steady-state levels of endogenous ROS and cell cycle progression through continued activity of APC in association with Cdh1.

scholarly journals A non-linear detection of phospho-histone H2AX in EA.hy926 endothelial cells following low-dose X-irradiation is modulated by reactive oxygen species

2014 ◽  
Vol 9 (1) ◽  
pp. 80 ◽  
Author(s):  
Martin Large ◽  
Sebastian Reichert ◽  
Stephanie Hehlgans ◽  
Claudia Fournier ◽  
Claus Rödel ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Low Dose ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Histone H2ax ◽  
Linear Detection ◽  
Non Linear ◽  
X Irradiation

A Chemical Perspective on the Interplay Between NO, Reactive Oxygen Species, and Reactive Nitrogen Oxide Species

2002 ◽  
Vol 962 (1) ◽  
pp. 195-206 ◽  
Author(s):  
MICHAEL G. ESPEY ◽  
KATRINA M. MIRANDA ◽  
DOUGLAS D. THOMAS ◽  
SANDHYA XAVIER ◽  
DEBORAH CITRIN ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Nitrogen Oxide ◽  
Reactive Oxygen ◽  
Reactive Nitrogen ◽  
Oxygen Species ◽  
Reactive Nitrogen Oxide Species
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