scholarly journals Haspin: A Mitotic Histone Kinase Required for Metaphase Chromosome Alignment

Cell Cycle ◽  
2005 ◽  
Vol 4 (5) ◽  
pp. 665-668 ◽  
Author(s):  
Jun Dai ◽  
Jonathan M.G. Higgins
Keyword(s):  
Metaphase Chromosome ◽  
Chromosome Alignment ◽  
Histone Kinase

scholarly journals Aurora B Regulates Formin mDia3 in Achieving Metaphase Chromosome Alignment

2011 ◽  
Vol 20 (3) ◽  
pp. 342-352 ◽  
Author(s):  
Lina Cheng ◽  
Jiayin Zhang ◽  
Sana Ahmad ◽  
Lorene Rozier ◽  
Haiqian Yu ◽  
...  
Keyword(s):  
Metaphase Chromosome ◽  
Aurora B ◽  
Chromosome Alignment

scholarly journals BubR1 and APC/EB1 cooperate to maintain metaphase chromosome alignment

2007 ◽  
Vol 179 (2) ◽  
pp. 357-357
Author(s):  
Jiayin Zhang ◽  
Sana Ahmad ◽  
Yinghui Mao
Keyword(s):  
Metaphase Chromosome ◽  
Chromosome Alignment

scholarly journals CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila

2000 ◽  
Vol 150 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Jennifer K. Yucel ◽  
Janet D. Marszalek ◽  
J. Richard McIntosh ◽  
Lawrence S.B. Goldstein ◽  
Don W. Cleveland ◽  
...  
Keyword(s):  
Metaphase Chromosome ◽  
Metaphase Plate ◽  
Pupal Stage ◽  
Complete Removal ◽  
P Element ◽  
Drosophila Cell ◽  
Chromosome Congression ◽  
Chromosome Alignment ◽  
Kinetochore Region ◽  
Female Germline

CENP-meta has been identified as an essential, kinesin-like motor protein in Drosophila. The 257-kD CENP-meta protein is most similar to the vertebrate kinetochore-associated kinesin-like protein CENP-E, and like CENP-E, is shown to be a component of centromeric/kinetochore regions of Drosophila chromosomes. However, unlike CENP-E, which leaves the centromere/kinetochore region at the end of anaphase A, the CENP-meta protein remains associated with the centromeric/kinetochore region of the chromosome during all stages of the Drosophila cell cycle. P-element–mediated disruption of the CENP-meta gene leads to late larval/pupal stage lethality with incomplete chromosome alignment at metaphase. Complete removal of CENP-meta from the female germline leads to lethality in early embryos resulting from defects in metaphase chromosome alignment. Real-time imaging of these mutants with GFP-labeled chromosomes demonstrates that CENP-meta is required for the maintenance of chromosomes at the metaphase plate, demonstrating that the functions required to establish and maintain chromosome congression have distinguishable requirements.

scholarly journals Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Mihaela Jagrić ◽  
Patrik Risteski ◽  
Jelena Martinčić ◽  
Ana Milas ◽  
Iva M Tolić
Keyword(s):  
Metaphase Chromosome ◽  
Mechanical Coupling ◽  
Chromosome Position ◽  
Sister Kinetochore ◽  
Chromosome Alignment ◽  
Bridging Fibers ◽  
Optogenetic Control

During metaphase, chromosome position at the spindle equator is regulated by the forces exerted by kinetochore microtubules and polar ejection forces. However, the role of forces arising from mechanical coupling of sister kinetochore fibers with bridging fibers in chromosome alignment is unknown. Here we develop an optogenetic approach for acute removal of PRC1 to partially disassemble bridging fibers and show that they promote chromosome alignment. Tracking of the plus-end protein EB3 revealed longer antiparallel overlaps of bridging microtubules upon PRC1 removal, which was accompanied by misaligned and lagging kinetochores. Kif4A/kinesin-4 and Kif18A/kinesin-8 were found within the bridging fiber and largely lost upon PRC1 removal, suggesting that these proteins regulate the overlap length of bridging microtubules. We propose that PRC1-mediated crosslinking of bridging microtubules and recruitment of kinesins to the bridging fiber promotes chromosome alignment by overlap length-dependent forces transmitted to the associated kinetochore fibers.

scholarly journals Tarun Kapoor: In the right position to study chromosomes

2009 ◽  
Vol 187 (6) ◽  
pp. 754-755
Author(s):  
Caitlin Sedwick
Keyword(s):  
High Resolution ◽  
Small Molecule ◽  
Metaphase Chromosome ◽  
Small Molecule Inhibitors ◽  
Chromosome Alignment ◽  
The Right ◽  
High Resolution Microscopy

Kapoor uses small molecule inhibitors and high-resolution microscopy to explore metaphase chromosome alignment.

scholarly journals The MRN-CtIP Pathway Is Required for Metaphase Chromosome Alignment

2013 ◽  
Vol 49 (6) ◽  
pp. 1097-1107 ◽  
Author(s):  
Lorene Rozier ◽  
Yige Guo ◽  
Shaun Peterson ◽  
Mai Sato ◽  
Richard Baer ◽  
...  
Keyword(s):  
Metaphase Chromosome ◽  
Chromosome Alignment

scholarly journals Haspin inhibitors reveal centromeric functions of Aurora B in chromosome segregation

2012 ◽  
Vol 199 (2) ◽  
pp. 251-268 ◽  
Author(s):  
Fangwei Wang ◽  
Natalia P. Ulyanova ◽  
John R. Daum ◽  
Debasis Patnaik ◽  
Anna V. Kateneva ◽  
...  
Keyword(s):  
Chromosome Segregation ◽  
Metaphase Chromosome ◽  
Histone H3 ◽  
Spindle Checkpoint ◽  
Aurora B ◽  
Docking Site ◽  
Chromosome Movement ◽  
Checkpoint Signaling ◽  
Chromosome Alignment ◽  
Spindle Midzone

Haspin phosphorylates histone H3 at threonine-3 (H3T3ph), providing a docking site for the Aurora B complex at centromeres. Aurora B functions to correct improper kinetochore–microtubule attachments and alert the spindle checkpoint to the presence of misaligned chromosomes. We show that Haspin inhibitors decreased H3T3ph, resulting in loss of centromeric Aurora B and reduced phosphorylation of centromere and kinetochore Aurora B substrates. Consequently, metaphase chromosome alignment and spindle checkpoint signaling were compromised. These effects were phenocopied by microinjection of anti-H3T3ph antibodies. Retargeting Aurora B to centromeres partially restored checkpoint signaling and Aurora B–dependent phosphorylation at centromeres and kinetochores, bypassing the need for Haspin activity. Haspin inhibitors did not obviously affect phosphorylation of histone H3 at serine-10 (H3S10ph) by Aurora B on chromosome arms but, in Aurora B reactivation assays, recovery of H3S10ph was delayed. Haspin inhibitors did not block Aurora B localization to the spindle midzone in anaphase or Aurora B function in cytokinesis. Thus, Haspin inhibitors reveal centromeric roles of Aurora B in chromosome movement and spindle checkpoint signaling.

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