scholarly journals Direct BMP2/4 signaling through BMP receptor IA regulates fetal thymocyte progenitor homeostasis and differentiation to CD4+CD8+ double-positive cell

Cell Cycle ◽  
2013 ◽  
Vol 13 (2) ◽  
pp. 324-333 ◽  
Author(s):  
Ariadne Hager-Theodorides ◽  
Susan Ross ◽  
Hemant Sahni ◽  
Yuji Mishina ◽  
Anna Furmanski ◽  
...  
Keyword(s):  
Double Positive ◽  
Bmp Receptor ◽  
Double Positive Cell ◽  
Fetal Thymocyte

scholarly journals CD33+/p-STAT1+ double-positive cell as a prognostic factor for stage IIIa gastric cancer

2013 ◽  
Vol 30 (1) ◽  
Author(s):  
Jun Dong ◽  
Jiao Li ◽  
Shi-Ming Liu ◽  
Xing-Yu Feng ◽  
Shi Chen ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Prognostic Factor ◽  
Stage Iiia ◽  
Double Positive ◽  
Double Positive Cell

Establishment and characterization of tartrate-resistant acid phosphatase and alkaline phosphatase double positive cell lines

2001 ◽  
Vol 304 (3) ◽  
pp. 351-359 ◽  
Author(s):  
Riako Masuda ◽  
Hisako Sakiyama ◽  
Takashi Nonaka ◽  
Alvin Kwan ◽  
Koichi Nakagawa ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Cell Lines ◽  
Tartrate Resistant Acid Phosphatase ◽  
Double Positive ◽  
Double Positive Cell

scholarly journals 652 Anti-PD-L1/IL-15 KD033 activated macrophages and induced anti-tumor immunity in the tumor-microenvironment

2021 ◽  
Vol 9 (Suppl 3) ◽  
pp. A681-A681
Author(s):  
Stella Martomo ◽  
George Wang ◽  
Zhanna Polonskaya ◽  
Xenia Luna ◽  
Dan Lu ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Transgenic Mice ◽  
Tumor Immunity ◽  
Immune Cells ◽  
Immune Gene ◽  
List Type ◽  
Double Positive ◽  
Double Positive Cell ◽  
Animal Care

BackgroundKD033 is a clinical-stage bispecific fusion molecule consisting of a high-affinity anti-human-PD-L1 antibody and human IL-15. Previous studies with KD033 in mice expressing functional human PD-1 and PD-L1 showed that KD033 was efficacious in reducing the growth of both PD-L1 positive and negative tumors.1 In the microenvironment of PD-L1 negative tumors, PD-L1 would still be expressed by some immune cells such as macrophages. The goal of the current study is to evaluate direct effect of KD033 on macrophages through in vitro studies and assess its contribution to anti-tumor immunity.MethodsMonocyte-derived human macrophages were treated with either KD033, the non-targeting IL15 fusion counterpart (ntKD033) or anti-PD-L1 antibody in vitro, and the supernatants were analyzed for cytokine/chemokine secretion. Human-PD-1/PD-L1 transgenic C57BL/6 mice subcutaneously transplanted with the human-PD-L1 positive or negative MC38 colon carcinoma were treated with KD033 intravenously when tumors reached 100 mm3. Tumor infiltrating cell populations were evaluated with Immunohistochemistry (IHC).ResultsIn vitro cultures with KD033 induced macrophages to secrete inflammatory cytokines such as IFNγ to a much higher level compared to ntKD033 cultures at the same concentrations. The anti-PD-L1 antibody alone did not induce macrophages to secrete IFNγ. IHC on KD033-treated human PD-1/PD-L1 transgenic mice showed that PD-L1 negative and positive MC38 tumors have similar levels of CD8 T cell tumor infiltrations. IHC with the macrophage marker CD68 showed higher level of CD68/IFNγ double positive cell infiltrations on PD-L1 negative tumors that was correlated with increased tumor growth inhibitions.ConclusionsIncreased in vitro IFNγ secretion from KD033-treated macrophages correlated with increased CD68/IFNγ double positive cell infiltrations in PD-L1 negative MC38 tumors from KD033-treated human PD-1/PD-L1 transgenic mice as evaluated by IHC. We hypothesized that our anti-PD-L1/IL15 KD033 induces anti-tumor immunity in PD-L1 negative tumors by activating PD-L1-expressing immune cells such as macrophages in the tumor microenvironment.Reference1. Martomo S, et al. Single-Dose anti–PD-L1/IL-15 fusion protein KD033 generates synergistic antitumor immunity with robust tumor-immune gene signatures and memory responses. Mol Cancer Ther 2021;20(2):347–356.Ethics ApprovalMouse studies were conducted for Kadmon by Wuxi AppTec Inc. All the procedures related to animal handling, care and the treatment in the study were performed according to the guidelines approved by the Institutional Animal Care and Use Committee (IACUC) of WuXi AppTec following the guidance of the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC).

Double-Positive Cell

2016 ◽  
Author(s):  
Douglas M. Templeton ◽  
Michael Schwenk ◽  
Reinhild Klein ◽  
John H. Duffus
Keyword(s):  
Double Positive ◽  
Double Positive Cell

Automated cell counting of astrocytes on patterned substrates containing aliphatic and charged properties

1995 ◽  
Vol 53 ◽  
pp. 974-975
Author(s):  
W. Shain ◽  
H. Ancin ◽  
H.C. Craighead ◽  
M. Isaacson ◽  
L. Kam ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Attachment ◽  
Culture Method ◽  
Cell Counting ◽  
Data Sets ◽  
Nuclear Staining ◽  
Double Positive ◽  
A Cell ◽  
Wafer Test ◽  
Cell Densities

Neural protheses have potential to restore nervous system functions lost by trauma or disease. Nanofabrication extends this approach to implants for stimulating and recording from single or small groups of neurons in the spinal cord and brain; however, tissue compatibility is a major limitation to their practical application. We are using a cell culture method for quantitatively measuring cell attachment to surfaces designed for nanofabricated neural prostheses.Silicon wafer test surfaces composed of 50-μm bars separated by aliphatic regions were fabricated using methods similar to a procedure described by Kleinfeld et al. Test surfaces contained either a single or double positive charge/residue. Cyanine dyes (diIC18(3)) stained the background and cell membranes (Fig 1); however, identification of individual cells at higher densities was difficult (Fig 2). Nuclear staining with acriflavine allowed discrimination of individual cells and permitted automated counting of nuclei using 3-D data sets from the confocal microscope (Fig 3). For cell attachment assays, LRM5 5 astroglial cells and astrocytes in primary cell culture were plated at increasing cell densities on test substrates, incubated for 24 hr, fixed, stained, mounted on coverslips, and imaged with a 10x objective.

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