scholarly journals Identification of in vitro HSC fate regulators by differential lipid raft clustering

Cell Cycle ◽  
2012 ◽  
Vol 11 (8) ◽  
pp. 1535-1543 ◽  
Author(s):  
Nicola Vannini ◽  
Aline Roch ◽  
Olaia Naveiras ◽  
Alessandra Griffa ◽  
Stefan Kobel ◽  
...  
Keyword(s):  
Lipid Raft ◽  
Raft Clustering

scholarly journals Actin Depolymerization Transduces the Strength of B-Cell Receptor Stimulation

2005 ◽  
Vol 16 (5) ◽  
pp. 2275-2284 ◽  
Author(s):  
Shengli Hao ◽  
Avery August
Keyword(s):  
B Cell ◽  
Lipid Raft ◽  
Cell Activation ◽  
Cell Receptor ◽  
Dependent Manner ◽  
B Cell Activation ◽  
Erk Activation ◽  
Actin Depolymerization ◽  
Transcription Factor Activation ◽  
Raft Clustering

Polymerization of the actin cytoskeleton has been found to be essential for B-cell activation. We show here, however, that stimulation of BCR induces a rapid global actin depolymerization in a BCR signal strength-dependent manner, followed by polarized actin repolymerization. Depolymerization of actin enhances and blocking actin depolymerization inhibits BCR signaling, leading to altered BCR and lipid raft clustering, ERK activation, and transcription factor activation. Furthermore actin depolymerization by itself induces altered lipid raft clustering and ERK activation, suggesting that F-actin may play a role in separating lipid rafts and in setting the threshold for cellular activation.

scholarly journals CD82 Restrains Pathological Angiogenesis by Altering Lipid Raft Clustering and CD44 Trafficking in Endothelial Cells

Circulation ◽  
2014 ◽  
Vol 130 (17) ◽  
pp. 1493-1504 ◽  
Author(s):  
Quan Wei ◽  
Feng Zhang ◽  
Mekel M. Richardson ◽  
Nathan H. Roy ◽  
William Rodgers ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Lipid Raft ◽  
Pathological Angiogenesis ◽  
Raft Clustering

The significance of integrin ligand nanopatterning on lipid raft clustering in hematopoietic stem cells

Biomaterials ◽  
2012 ◽  
Vol 33 (11) ◽  
pp. 3107-3118 ◽  
Author(s):  
Eva Altrock ◽  
Christine A. Muth ◽  
Gerd Klein ◽  
Joachim P. Spatz ◽  
Cornelia Lee-Thedieck
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Lipid Raft ◽  
Hematopoietic Stem ◽  
Integrin Ligand ◽  
Raft Clustering

scholarly journals Ca2+-regulated lysosome fusion mediates angiotensin II-induced lipid raft clustering in mesenteric endothelial cells

2016 ◽  
Vol 39 (4) ◽  
pp. 227-236 ◽  
Author(s):  
Wei-Qing Han ◽  
Wen-Dong Chen ◽  
Ke Zhang ◽  
Jian-Jun Liu ◽  
Yong-Jie Wu ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Angiotensin Ii ◽  
Lipid Raft ◽  
Raft Clustering

scholarly journals Regulation of Gap Junctions in Porcine Cumulus-Oocyte Complexes: Contributions of Granulosa Cell Contact, Gonadotropins, and Lipid Rafts

2009 ◽  
Vol 23 (5) ◽  
pp. 700-710 ◽  
Author(s):  
Maxime Sasseville ◽  
Marie-Claude Gagnon ◽  
Christine Guillemette ◽  
Robert Sullivan ◽  
Robert B. Gilchrist ◽  
...  
Keyword(s):  
Gap Junctions ◽  
Lipid Rafts ◽  
Lipid Raft ◽  
Connexin 43 ◽  
In Vitro Maturation ◽  
Cell Contact ◽  
Germinal Vesicle Breakdown ◽  
Oocyte Growth ◽  
Junctional Communication

Abstract Gap-junctional communication (GJC) plays a central role in oocyte growth. However, little is known about the regulation of connexin 43 (Cx43)-based gap-junction channels in cumulus-oocyte complexes (COCs) during in vitro maturation. We show that rupture of COCs from mural granulosa cells up-regulates Cx43-mediated GJC and that gonadotropins signal GJC breakdown by recruiting Cx43 to lipid rafts when oocyte meiosis resumes. Oocyte calcein uptake through gap junctions increases during early in vitro oocyte maturation and remains high until 18 h, when it falls simultaneously with the oocyte germinal vesicle breakdown. Immunodetection of Cx43 and fluorescence recovery after photobleaching assays revealed that the increase of GJC is independent of gonadotropins but requires RNA transcription, RNA polyadenylation, and translation. GJC rupture, in contrast, is achieved by a gonadotropin-dependent mechanism involving recruitment of Cx43 to clustered lipid rafts. These results show that GJC up-regulation in COCs in in vitro culture is independent of gonadotropins and transcriptionally regulated. However, GJC breakdown is gonadotropin dependent and mediated by the clustering of Cx43 in lipid raft microdomains. In conclusion, this study supports a functional role of lipid raft clustering of Cx43 in GJC breakdown in the COCs during in vitro maturation.

Immunoliposomes That Target Endothelium In Vitro Are Dependent on Lipid Raft Formation

2010 ◽  
Vol 7 (5) ◽  
pp. 1569-1575 ◽  
Author(s):  
Rico C. Gunawan ◽  
Debra T. Auguste
Keyword(s):  
Lipid Raft

scholarly journals Rnd1 and Rnd3 targeting to lipid raft is required for p190 RhoGAP activation

2012 ◽  
Vol 23 (8) ◽  
pp. 1593-1604 ◽  
Author(s):  
Izumi Oinuma ◽  
Kana Kawada ◽  
Kiyoka Tsukagoshi ◽  
Manabu Negishi
Keyword(s):  
Lipid Raft ◽  
Cell Types ◽  
Functional Difference ◽  
Distinct Subgroup ◽  
Functional Differences ◽  
P190 Rhogap ◽  
Rho Family ◽  
Rhoa Signaling

The Rnd proteins Rnd1, Rnd2, and Rnd3/RhoE are well known as key regulators of the actin cytoskeleton in various cell types, but they comprise a distinct subgroup of the Rho family in that they are GTP bound and constitutively active. Functional differences of the Rnd proteins in RhoA inhibition signaling have been reported in various cell types. Rnd1 and Rnd3 antagonize RhoA signaling by activating p190 RhoGAP, whereas Rnd2 does not. However, all the members of the Rnd family have been reported to bind directly to p190 RhoGAP and equally induce activation of p190 RhoGAP in vitro, and there is no evidence that accounts for the functional difference of the Rnd proteins in RhoA inhibition signaling. Here we report the role of the N-terminal region in signaling. Rnd1 and Rnd3, but not Rnd2, have a KERRA (Lys-Glu-Arg-Arg-Ala) sequence of amino acids in their N-terminus, which functions as the lipid raft-targeting determinant. The sequence mediates the lipid raft targeting of p190 RhoGAP correlated with its activation. Overall, our results demonstrate a novel regulatory mechanism by which differential membrane targeting governs activities of Rnd proteins to function as RhoA antagonists.

Cytokine Signaling, Lipid Raft Clustering, and HSC Hibernation

2007 ◽  
Vol 1106 (1) ◽  
pp. 54-63 ◽  
Author(s):  
S. YAMAZAKI ◽  
A. IWAMA ◽  
Y. MORITA ◽  
K. ETO ◽  
H. EMA ◽  
...  
Keyword(s):  
Lipid Raft ◽  
Cytokine Signaling ◽  
Raft Clustering
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