scholarly journals Lipid droplet formation protects against gluco/lipotoxicity inCandida parapsilosis: An essential role of fatty acid desaturase Ole1

Cell Cycle ◽  
2011 ◽  
Vol 10 (18) ◽  
pp. 3159-3167 ◽  
Author(s):  
Long Nam Nguyen ◽  
Joshua D. Nosanchuk
Keyword(s):  
Fatty Acid ◽  
Lipid Droplet ◽  
Essential Role ◽  
Fatty Acid Desaturase ◽  
Droplet Formation ◽  
Lipid Droplet Formation

Role of hepatic Annexin A6 in fatty acid-induced lipid droplet formation

2017 ◽  
Vol 358 (2) ◽  
pp. 397-410 ◽  
Author(s):  
Rose Cairns ◽  
Anna Alvarez-Guaita ◽  
Inés Martínez-Saludes ◽  
Sundeep J. Wason ◽  
Jacky Hanh ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Droplet ◽  
Droplet Formation ◽  
Lipid Droplet Formation

scholarly journals Role of Caveolin-1 in the Modulation of Lipolysis and Lipid Droplet Formation

Diabetes ◽  
2004 ◽  
Vol 53 (5) ◽  
pp. 1261-1270 ◽  
Author(s):  
A. W. Cohen ◽  
B. Razani ◽  
W. Schubert ◽  
T. M. Williams ◽  
X. B. Wang ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Droplet Formation ◽  
Caveolin 1 ◽  
Lipid Droplet Formation

scholarly journals The role of phosphatidylcholine transfer by STARD10 and synthesis by LPCAT1 in lipid droplet formation

2019 ◽  
Vol 92 (0) ◽  
pp. 1-O-14
Author(s):  
Masanori Ito ◽  
Taichiro Tomida ◽  
Yoshinori Mikami ◽  
Daisuke Ohshima ◽  
Satomi Adachi-Akahane
Keyword(s):  
Lipid Droplet ◽  
Droplet Formation ◽  
Lipid Droplet Formation

Seipin deficiency alters fatty acid Δ9 desaturation and lipid droplet formation in Berardinelli-Seip congenital lipodystrophy

Biochimie ◽  
2009 ◽  
Vol 91 (6) ◽  
pp. 796-803 ◽  
Author(s):  
Emilie Boutet ◽  
Haquima El Mourabit ◽  
Matthieu Prot ◽  
Mona Nemani ◽  
Eliane Khallouf ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Droplet ◽  
Droplet Formation ◽  
Lipid Droplet Formation

Flow cytometric analysis of lipid droplet formation in cells of the human monocytic cell line, U937

1991 ◽  
Vol 69 (8) ◽  
pp. 571-576 ◽  
Author(s):  
K. Suzuki ◽  
N. Sakata ◽  
M. Hara ◽  
A. Kitani ◽  
M. Harigai ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Cell Line ◽  
Lipid Droplet ◽  
Droplet Formation ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Human Monocytic Cell Line ◽  
Lipid Droplet Formation ◽  
Human Monocytic Cell

Active uptake of a free fatty acid, oleate, and its incorporation into triacylglycerol and phospholipid in the human monocytic cell line, U937, was identified using 14C-labelled oleate. Excess triacylglycerol accumulates in the form of lipid droplets in the cytoplasm. The extent of lipid droplet formation in each cell can be assessed in the absence of any staining by 90° light scatter intensity, one of the basic parameters of flow cytometry.Key words: monocyte, free fatty acid, triacylglycerol, flow cytometry.

ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts

2002 ◽  
Vol 283 (4) ◽  
pp. E775-E783 ◽  
Author(s):  
Minako Imamura ◽  
Toyoshi Inoguchi ◽  
Shoichiro Ikuyama ◽  
Susumu Taniguchi ◽  
Kunihisa Kobayashi ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Droplet ◽  
Lipid Accumulation ◽  
Lipid Droplets ◽  
Droplet Formation ◽  
Fatty Acid Binding ◽  
Lipogenic Genes ◽  
Lipid Droplet Formation ◽  
Murine Fibroblasts ◽  
Adipose Differentiation

Adipose differentiation-related protein (ADRP) is a lipid droplet-associated protein that is expressed early during adipose differentiation. The present study was undertaken to reveal the role of ADRP in adipose differentiation. In murine fibroblasts infected with green fluorescent protein (GFP)-ADRP fusion protein expression adenovirus vector, confocal microscopic analysis showed the number and size of lipid droplets apparently increased comparing with those of control cells. Overexpressed GFP-ADRP were mainly located at the surface of lipid droplets and appeared to be “ring-shaped.” Triacylglycerol content was also significantly ( P < 0.001) increased in GFP-ADRP-overexpressed cells compared with control cells. ADRP-induced lipid accumulation did not depend on adipocyte-specific gene induction, such as peroxisome proliferator-activated receptor-γ, lipoprotein lipase, or other lipogenic genes, including acyl-CoA synthetase, fatty acid-binding protein, and fatty acid transporter. In conclusion, ADRP stimulated lipid accumulation and lipid droplet formation without induction of other adipocyte-specific genes or other lipogenic genes in murine fibroblasts. The detailed molecular mechanisms of ADRP on lipid accumulation remain to be elucidated.

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