scholarly journals Primary tumor cells obtained from MNU-induced mammary carcinomas show immune heterogeneity which can be modulated by low-efficiency transfection of CD40L gene

2009 ◽  
Vol 8 (2) ◽  
pp. 136-142 ◽  
Author(s):  
Gunes Esendagli ◽  
Hande Canpinar ◽  
Guldal Yilmaz ◽  
Aysen Gunel-Ozcan ◽  
M. Oguz Guc ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Primary Tumor ◽  
Mammary Carcinomas ◽  
Low Efficiency

scholarly journals Metastatic Esophageal Carcinoma Cells Exhibit Reduced Adhesion Strength and Enhanced Thermogenesis

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1213
Author(s):  
Zihe Huo ◽  
Mariana Sá Santos ◽  
Astrid Drenckhan ◽  
Stefan Holland-Cunz ◽  
Jakob R. Izbicki ◽  
...  
Keyword(s):  
Esophageal Carcinoma ◽  
Tumor Cells ◽  
Cell Lines ◽  
Adhesion Strength ◽  
Primary Tumor ◽  
Carcinoma Cell ◽  
Metastatic Potential ◽  
Primary Tumors ◽  
Metastatic Cell ◽  
Carcinoma Cell Lines

Despite continuous improvements in multimodal therapeutic strategies, esophageal carcinoma maintains a high mortality rate. Metastases are a major life-limiting component; however, very little is known about why some tumors have high metastatic potential and others not. In this study, we investigated thermogenic activity and adhesion strength of primary tumor cells and corresponding metastatic cell lines derived from two patients with metastatic adenocarcinoma of the esophagus. We hypothesized that the increased metastatic potential of the metastatic cell lines correlates with higher thermogenic activity and decreased adhesion strength. Our data show that patient-derived metastatic esophageal tumor cells have a higher thermogenic profile as well as a decreased adhesion strength compared to their corresponding primary tumor cells. Using two paired esophageal carcinoma cell lines of primary tumor and lymph nodes makes the data unique. Both higher specific thermogenesis profile and decreased adhesion strength are associated with a higher metastatic potential. They are in congruence with the clinical patient presentation. Understanding these functional, biophysical properties of patient derived esophageal carcinoma cell lines will enable us to gain further insight into the mechanisms of metastatic potential of primary tumors and metastases. Microcalorimetric evaluation will furthermore allow for rapid assessment of new treatment options for primary tumor and metastases aimed at decreasing the metastatic potential.

Autophagy-inhibiting biomimetic nanodrugs enhance photothermal therapy and boost antitumor immunity

2022 ◽  
Author(s):  
Peiying Huang ◽  
Yin Yin Zhu ◽  
Hao Zhong ◽  
Peiling Chen ◽  
Qunying Shi ◽  
...  
Keyword(s):  
Low Temperature ◽  
Tumor Cells ◽  
Stress Responses ◽  
Photothermal Therapy ◽  
Tumor Recurrence ◽  
Antitumor Immunity ◽  
Blood Clearance ◽  
Low Efficiency

The instinctive protective stress responses of tumor cells hamper the low-temperature photothermal therapy (LTPTT), resulting in tumor recurrence and metastasis. The rapid blood clearance and low-efficiency tumor enrichment of nanomedicines...

Methods to Study Primary Tumor Cells and Residual Tumor Cells in Mouse Models of Oncogene Dependence

2015 ◽  
pp. 381-394
Author(s):  
Caroline Botta ◽  
Cedric Darini ◽  
Guillaume Darrasse-Jèze ◽  
Katrina Podsypanina
Keyword(s):  
Tumor Cells ◽  
Mouse Models ◽  
Primary Tumor ◽  
Residual Tumor

scholarly journals Configuration and expression of the T cell receptor beta chain gene in human T-lymphotrophic virus I-infected cells.

1986 ◽  
Vol 163 (2) ◽  
pp. 383-399 ◽  
Author(s):  
R F Jarrett ◽  
H Mitsuya ◽  
D L Mann ◽  
J Cossman ◽  
S Broder ◽  
...  
Keyword(s):  
T Cell ◽  
Immune Function ◽  
Tumor Cells ◽  
T Cell Receptor ◽  
Cell Lines ◽  
Primary Tumor ◽  
Gene Rearrangement ◽  
Cell Receptor ◽  
Beta Chain ◽  
Beta Gene

We studied the configuration and expression of the gene encoding the beta chain of the T cell receptor (TCR beta) in cell lines and primary tumor cells infected by the human T cell leukemia/lymphoma (lymphotrophic) virus type I (HTLV-I). Most of the cell lines and all the primary tumor cells showed rearrangement of the TCR beta gene, and in each case the rearrangement was distinct. The majority of cases examined were clonal with respect to a particular TCR beta gene rearrangement. Primary tumor cells from one case (SD) were found to have a tandem duplication of a portion of chromosome 7; this appears to have resulted in the presence of three alleles of the TCR beta gene, each of which is arranged differently. This suggests that the chromosomal abnormality, and possibly infection by HTLV-I, occurred before TCR beta gene rearrangement. Cell lines infected by HTLV-I express levels of TCR beta mRNA similar to PHA stimulated lymphocytes, suggesting that this gene is not transcriptionally activated as a result of infection by HTLV-I. Cloned T cells of known antigen specificity that are infected by HTLV-I in vitro show impairment of immune function, including loss of antigen-specific responsiveness and the acquisition of alloreactivity. Comparison of the configuration of the TCR beta gene before and after infection revealed no changes detectable by Southern blot analysis. Levels of expression of the TCR beta gene at the mRNA level and surface expression of the T3 complex were also not significantly altered, suggesting that changes in immune function cannot be attributed to quantitative changes in the TCR molecule. The configuration of the TCR beta gene in primary tumor cells infected by HTLV-I was compared with that in the derived cell lines. In all pairs examined, the configuration in the primary tumor cells was different from that in the cell lines, strongly suggesting that the cells that grow in culture are not the original neoplastic cells.

scholarly journals Characteristics of the Epithelial-Mesenchymal Transition in Primary and Paired Metastatic Canine Mammary Carcinomas

2018 ◽  
Vol 55 (5) ◽  
pp. 622-633 ◽  
Author(s):  
Talita M. M. Raposo-Ferreira ◽  
Becky K. Brisson ◽  
Amy C. Durham ◽  
Renee Laufer-Amorim ◽  
Veronica Kristiansen ◽  
...  
Keyword(s):  
Primary Tumor ◽  
Epithelial Mesenchymal Transition ◽  
Critical Role ◽  
Cell Counting ◽  
Positive Staining ◽  
Primary Tumors ◽  
Mesenchymal Transition ◽  
Mammary Carcinomas ◽  
Slug Expression ◽  
E Cadherin

The epithelial-mesenchymal transition (EMT) is a dynamic process linked to metastasis in many tumor types, including mammary tumors. In this study, we evaluated E-cadherin and vimentin immunolocalization in primary canine mammary carcinomas (20 cases) and their respective metastases, as well as their relationship with the core regulators SNAIL/SLUG. To assess the number of cells undergoing the process of EMT, we quantitated double-positive (E-cadherin+/vimentin+) cells using immunofluorescence, via cell counting and image analysis. In addition, SNAIL/SLUG expression was evaluated by established immunohistochemical methods. Primary tumors had significantly more E-cadherin+/vimentin+ co-expression than their paired respective lymph node or distant metastasis, respectively. Furthermore, the percentage of E-cadherin+/vimentin+ cells in grade II and III carcinomas was significantly higher than in grade I tumors. Primary tumors had significantly higher SNAIL/SLUG expression when analyzed based on the percentage of positive cells compared with their respective distant metastases in pairwise comparisons. An inverse correlation was noted between SNAIL/SLUG immunoreactivity and percentage of E-cadherin+/vimentin+ immunopositive cells in primary tumor samples when SNAIL/SLUG immunoreactivity was grouped into 2 categories (high versus low) based on percentage-positive staining. These results show a positive correlation between E-cadherin+/vimentin+ cells and higher tumor grade, establish differences between primary tumor and their respective metastases, and provide further support that EMT plays a critical role in the metastasis of canine mammary carcinoma. Furthermore, these data suggest that modulation of this process could provide greater therapeutic control and provide support for further research to determine if E-cadherin+/vimentin+ co-immunoreactivity imparts predictive value in the clinical outcome of patients with canine mammary carcinomas.

scholarly journals Survival Impact of Primary Tumor Lymph Node Status and Circulating Tumor Cells in Patients with Colorectal Liver Metastases

2017 ◽  
Vol 24 (8) ◽  
pp. 2113-2121 ◽  
Author(s):  
Lars Thomas Seeberg ◽  
Cathrine Brunborg ◽  
Anne Waage ◽  
Harald Hugenschmidt ◽  
Anne Renolen ◽  
...  
Keyword(s):  
Lymph Node ◽  
Liver Metastases ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Primary Tumor ◽  
Colorectal Liver Metastases ◽  
Lymph Node Status ◽  
Node Status
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