scholarly journals Bioengineered heparin

Bioengineered ◽  
2014 ◽  
Vol 5 (4) ◽  
pp. 222-226 ◽  
Author(s):  
Megan S Lord ◽  
John M Whitelock
Keyword(s):  
Bioengineered Heparin

scholarly journals High cell density cultivation of a recombinant E. coli strain expressing a key enzyme in bioengineered heparin production

2013 ◽  
Vol 97 (9) ◽  
pp. 3893-3900 ◽  
Author(s):  
Odile Francesca Restaino ◽  
Ujjwal Bhaskar ◽  
Priscilla Paul ◽  
Lingyun Li ◽  
Mario De Rosa ◽  
...  
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Coli Strain ◽  
High Cell ◽  
High Cell Density Cultivation ◽  
E Coli ◽  
Bioengineered Heparin ◽  
Key Enzyme

scholarly journals E. coli K5 fermentation and the preparation of heparosan, a bioengineered heparin precursor

2010 ◽  
Vol 107 (6) ◽  
pp. 964-973 ◽  
Author(s):  
Zhenyu Wang ◽  
Mellisa Ly ◽  
Fuming Zhang ◽  
Weihong Zhong ◽  
Amy Suen ◽  
...  
Keyword(s):  
E Coli ◽  
Bioengineered Heparin

scholarly journals Controlled photochemical depolymerization of K5 heparosan, a bioengineered heparin precursor

2011 ◽  
Vol 86 (3) ◽  
pp. 1365-1370 ◽  
Author(s):  
Kyohei Higashi ◽  
Mellisa Ly ◽  
Zhenyu Wang ◽  
Sayaka Masuko ◽  
Ujjwal Bhaskar ◽  
...  
Keyword(s):  
Bioengineered Heparin

scholarly journals Library of Recombinant Heparan Sulfates from Mammalian Cell Culture: Paving the Way to Bioengineered Heparin

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Bryan Thacker ◽  
Gloria Lee ◽  
Max Scott ◽  
Tobin Groth ◽  
Kristen Thorne ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Bioengineered Heparin ◽  
Heparan Sulfates ◽  
The Way

scholarly journals Comparative Pharmacological Profiles of Various Bovine, Ovine, and Porcine Heparins

2019 ◽  
Vol 25 ◽  
pp. 107602961988940 ◽  
Author(s):  
Ahmed Kouta ◽  
Walter Jeske ◽  
Debra Hoppensteadt ◽  
Omer Iqbal ◽  
Yiming Yao ◽  
...  
Keyword(s):  
Vascular System ◽  
Mammalian Species ◽  
Regulatory Agencies ◽  
Low Molecular Weight ◽  
Low Molecular Weight Heparins ◽  
Indwelling Catheters ◽  
Bioengineered Heparin ◽  
Anticoagulant Drug ◽  
Alternative Sources ◽  
Religious Reasons

Unfractionated heparin is the first anticoagulant drug and has been successfully used clinically for over 80 years. Heparin and its analogues are used during surgery and dialysis and are often used to coat indwelling catheters and other devices where the vascular system is exposed. Most of the heparins used clinically are derived from porcine intestinal mucosa. However, heparins have also been manufactured from tissues of other mammalian species such as cows and sheep. Recently there have been attempts to generate bioengineered heparin in order to overcome contamination and antigenicity problems. Currently there are some concerns about the shortage of the porcine heparins as they are widely used in the manufacturing of the low-molecular-weight heparins. Moreover, due to cultural and religious reasons in some countries, alternative sources of heparins are needed. The Food and Drug Administration and other regulatory agencies have considered alternative sourcing of heparin for potential substitution of porcine heparin and are currently reviewing this matter. Numerous studies are ongoing to understand the structure-activity relationships of these various heparins. In this article, heparins from different animal sources were studied to determine the extent of biosimilarity between them. For these investigations, 10 batches each of bovine mucosal heparin (BMH), ovine mucosal heparin (OMH), and porcine mucosal heparin (PMH) were studied. These studies have demonstrated that OMH and PMH have comparable anticoagulant and antiproteases activities. However, BMH exhibited somewhat a lower potency compared to OMH and PMH in functional assays.

Addressing endotoxin issues in bioengineered heparin

2012 ◽  
Vol 59 (6) ◽  
pp. 420-428 ◽  
Author(s):  
Jiraporn Suwan ◽  
Amanda Torelli ◽  
Akihiro Onishi ◽  
Jonathan S. Dordick ◽  
Robert J. Linhardt
Keyword(s):  
Bioengineered Heparin

scholarly journals Optimization of bioprocess conditions improves production of a CHO cell-derived, bioengineered heparin

2015 ◽  
Vol 10 (7) ◽  
pp. 1067-1081 ◽  
Author(s):  
Jong Youn Baik ◽  
Hussain Dahodwala ◽  
Eziafa Oduah ◽  
Lee Talman ◽  
Trent R. Gemmill ◽  
...  
Keyword(s):  
Cho Cell ◽  
Bioengineered Heparin

scholarly journals Toward a bioengineered heparin

Bioengineered ◽  
2012 ◽  
Vol 3 (4) ◽  
pp. 227-231 ◽  
Author(s):  
Jong Youn Baik ◽  
Clifford L. Wang ◽  
Bo Yang ◽  
Robert J. Linhardt ◽  
Susan T. Sharfstein
Keyword(s):  
Bioengineered Heparin
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