scholarly journals Novel strategies to improve co-fermentation of pentoses with D-glucose by recombinant yeast strains in lignocellulosic hydrolysates

Bioengineered ◽  
2012 ◽  
Vol 3 (6) ◽  
pp. 347-351 ◽  
Author(s):  
Mislav Oreb ◽  
Heiko Dietz ◽  
Alexander Farwick ◽  
Eckhard Boles
Keyword(s):  
Recombinant Yeast ◽  
Lignocellulosic Hydrolysates ◽  
Yeast Strains ◽  
Recombinant Yeast Strains

scholarly journals Direct Production of Difructose Anhydride IV from Sucrose by Co-fermentation of Recombinant Yeasts

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hyunjun Ko ◽  
Jung-Hoon Bae ◽  
Bong Hyun Sung ◽  
Mi-Jin Kim ◽  
Soon-Ho Park ◽  
...  
Keyword(s):  
Fermentation Broth ◽  
Sucrose Concentration ◽  
Direct Conversion ◽  
Efficient Production ◽  
Recombinant Yeast ◽  
Direct Production ◽  
Yeast Strains ◽  
Secretory Production ◽  
Recombinant Yeast Strains ◽  
Recombinant Yeasts

Abstract A functional sweetener, difructose anhydride IV (DFA IV), is enzymatically produced from sucrose via levan by levansucrase (LSRase) followed by levan fructotransferase (LFTase). Here, we have demonstrated a consolidated production system for the direct conversion of DFA IV from sucrose using the co-culture of two recombinant yeast strains secreting LSRase from Bacillus subtilis and LFTase from Arthrobacter ureafaciens, respectively. To ensure secretory production of the enzymes, target protein-specific translational fusion partners (TFP) were employed, and the selected strains produced 3.8 U/mL of LSRase and 16.0 U/mL LFTase activity into the fermentation broth. To optimise the direct production, sucrose concentration and cell ratios were investigated. In the optimised conditions, 64.3 g/L crude DFA IV was directly produced from 244.7 g/L sucrose using co-fermentation of recombinant yeasts. These results promise an efficient production titre, yield, and DFA IV productivity in an industrially applicable method.

scholarly journals Revealing Complex Traits with Small Molecules and Naturally Recombinant Yeast Strains

2006 ◽  
Vol 13 (3) ◽  
pp. 319-327 ◽  
Author(s):  
Ethan O. Perlstein ◽  
Douglas M. Ruderfer ◽  
Gopal Ramachandran ◽  
Stephen J. Haggarty ◽  
Leonid Kruglyak ◽  
...  
Keyword(s):  
Small Molecules ◽  
Complex Traits ◽  
Recombinant Yeast ◽  
Yeast Strains ◽  
Recombinant Yeast Strains

scholarly journals The effects of statins on the mevalonic acid pathway in recombinant yeast strains expressing human HMG-CoA reductase

2013 ◽  
Vol 13 (1) ◽  
pp. 68 ◽  
Author(s):  
Agata Maciejak ◽  
Agata Leszczynska ◽  
Ilona Warchol ◽  
Monika Gora ◽  
Joanna Kaminska ◽  
...  
Keyword(s):  
Mevalonic Acid ◽  
Recombinant Yeast ◽  
Hmg Coa Reductase ◽  
Yeast Strains ◽  
Mevalonic Acid Pathway ◽  
Acid Pathway ◽  
Coa Reductase ◽  
Recombinant Yeast Strains

scholarly journals Yeast-Based Biosynthesis of Natural Products From Xylose

2021 ◽  
Vol 9 ◽  
Author(s):  
Jian Zha ◽  
Miaomiao Yuwen ◽  
Weidong Qian ◽  
Xia Wu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Natural Products ◽  
Metabolic Engineering ◽  
State Of The Art ◽  
Commercial Production ◽  
Scheffersomyces Stipitis ◽  
Lignocellulosic Hydrolysates ◽  
Yeast Strains ◽  
Future Challenges ◽  
Biomass Materials

Xylose is the second most abundant sugar in lignocellulosic hydrolysates. Transformation of xylose into valuable chemicals, such as plant natural products, is a feasible and sustainable route to industrializing biorefinery of biomass materials. Yeast strains, including Saccharomyces cerevisiae, Scheffersomyces stipitis, and Yarrowia lipolytica, display some paramount advantages in expressing heterologous enzymes and pathways from various sources and have been engineered extensively to produce natural products. In this review, we summarize the advances in the development of metabolically engineered yeasts to produce natural products from xylose, including aromatics, terpenoids, and flavonoids. The state-of-the-art metabolic engineering strategies and representative examples are reviewed. Future challenges and perspectives are also discussed on yeast engineering for commercial production of natural products using xylose as feedstocks.

Co-production of xylitol and ethanol by the fermentation of the lignocellulosic hydrolysates of banana and water hyacinth leaves by individual yeast strains

2020 ◽  
Vol 155 ◽  
pp. 112809
Author(s):  
Kumar Shankar ◽  
Neelkant S. Kulkarni ◽  
Rakesh Sajjanshetty ◽  
S.K. Jayalakshmi ◽  
Kuruba Sreeramulu
Keyword(s):  
Water Hyacinth ◽  
Lignocellulosic Hydrolysates ◽  
Yeast Strains

Yeast strains for ethanol production from lignocellulosic hydrolysates during in situ detoxification

2009 ◽  
Vol 27 (5) ◽  
pp. 656-660 ◽  
Author(s):  
Shen Tian ◽  
Guixiong Zhou ◽  
Fei Yan ◽  
Yong Yu ◽  
Xiushan Yang
Keyword(s):  
Ethanol Production ◽  
Lignocellulosic Hydrolysates ◽  
Yeast Strains ◽  
In Situ Detoxification

Effect of MIG1 Gene Deletion on Glucose Repression in Baker’s Yeast

2011 ◽  
Vol 396-398 ◽  
pp. 1531-1535
Author(s):  
Yan Zhang ◽  
Dong Guang Xiao ◽  
Cui Ying Zhang ◽  
Xi Sun ◽  
Ming Yue Wu
Keyword(s):  
Gene Deletion ◽  
Glucose Repression ◽  
Critical Role ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Maltose Metabolism ◽  
Yeast Strains ◽  
Maltose Utilization ◽  
Main Component ◽  
Recombinant Yeast Strains

Mig1p, a zinc finger class of DNA-binding protein, plays a critical role in glucose repression for maltose utilization in Baker’s yeast. Maltose is the hydrolyzate of starch, which is the main component of dough. In this study, the recombinant yeast strains with MIG1 gene deletion were constructed, and the maltose metabolism of the parental and mutant strains in batch cultivations were investigated. Our results show that the degree of glucose repression of mutants △MIG1α and △MIG1a are reduced by 49.88% and 41.59% respectively compared to their parental strains, suggesting that MIG1 deletion can partially relieve glucose repression of maltose metabolism.

Differential effects of major inhibitory compounds from sugarcane-based lignocellulosic hydrolysates on the physiology of yeast strains and lactic acid bacteria

2020 ◽  
Vol 42 (4) ◽  
pp. 571-582 ◽  
Author(s):  
Priscila Cola ◽  
Dielle Pierotti Procópio ◽  
Adriana Tabosa de Castro Alves ◽  
Luciana Rebelo Carnevalli ◽  
Icaro Viana Sampaio ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lignocellulosic Hydrolysates ◽  
Differential Effects ◽  
Yeast Strains ◽  
Inhibitory Compounds

scholarly journals Saccharomyces cerevisiae Cells Lacking the Zinc Vacuolar Transporter Zrt3 Display Improved Ethanol Productivity in Lignocellulosic Hydrolysates

2022 ◽  
Vol 8 (1) ◽  
pp. 78
Author(s):  
Joana Terra-Matos ◽  
Marta Oliveira Teixeira ◽  
Cátia Santos-Pereira ◽  
Henrique Noronha ◽  
Lucília Domingues ◽  
...  
Keyword(s):  
Ethanol Productivity ◽  
Biofuel Production ◽  
Yeast Fermentation ◽  
Lignocellulosic Ethanol ◽  
Wild Type ◽  
Lignocellulosic Hydrolysates ◽  
Promising Strategy ◽  
Yeast Strains ◽  
Regulated Cell Death ◽  
Fermentative Yeast

Yeast-based bioethanol production from lignocellulosic hydrolysates (LH) is an attractive and sustainable alternative for biofuel production. However, the presence of acetic acid (AA) in LH is still a major problem. Indeed, above certain concentrations, AA inhibits yeast fermentation and triggers a regulated cell death (RCD) process mediated by the mitochondria and vacuole. Understanding the mechanisms involved in AA-induced RCD (AA-RCD) may thus help select robust fermentative yeast strains, providing novel insights to improve lignocellulosic ethanol (LE) production. Herein, we hypothesized that zinc vacuolar transporters are involved in vacuole-mediated AA-RCD, since zinc enhances ethanol production and zinc-dependent catalase and superoxide dismutase protect from AA-RCD. In this work, zinc limitation sensitized wild-type cells to AA-RCD, while zinc supplementation resulted in a small protective effect. Cells lacking the vacuolar zinc transporter Zrt3 were highly resistant to AA-RCD, exhibiting reduced vacuolar dysfunction. Moreover, zrt3Δ cells displayed higher ethanol productivity than their wild-type counterparts, both when cultivated in rich medium with AA (0.29 g L−1 h−1 versus 0.11 g L−1 h−1) and in an LH (0.73 g L−1 h−1 versus 0.55 g L−1 h−1). Overall, the deletion of ZRT3 emerges as a promising strategy to increase strain robustness in LE industrial production.

scholarly journals Enhanced Susceptibility to Antifungal Oligopeptides in Yeast Strains Overexpressing ABC Multidrug Efflux Pumps

2008 ◽  
Vol 52 (11) ◽  
pp. 4057-4063 ◽  
Author(s):  
Roland Wakieć ◽  
Iwona Gabriel ◽  
Rajendra Prasad ◽  
Jeffrey M. Becker ◽  
John W. Payne ◽  
...  
Keyword(s):  
Abc Transporters ◽  
Efflux Pumps ◽  
Multidrug Resistant ◽  
Yeast Cells ◽  
Drug Efflux ◽  
Multidrug Efflux ◽  
Yeast Strains ◽  
Multidrug Efflux Pumps ◽  
Drug Efflux Pumps ◽  
Recombinant Yeast Strains

ABSTRACT The susceptibility to several oligopeptide and amino acid antifungals of a Saccharomyces cerevisiae strain carrying multiple deletions in yeast multidrug resistance genes was compared to transformants containing the CDR1, CDR2, or MDR1 genes that encode the major Candida albicans drug efflux pumps. Recombinant yeast strains overexpressing Cdr1p and Cdr2p showed enhanced susceptibilities to all tested oligopeptide antifungals. The enhanced susceptibilities of multidrug-resistant yeast strains to oligopeptide antifungals corresponded to higher rates of oligopeptide uptake. Yeast cells overexpressing Cdr1p or Cdr2p effluxed protons at higher rates than the reference cells lacking these ABC transporters. An increased plasma membrane electrochemical gradient caused by the functional overexpression of Cdr1p or Cdr2p appeared to increase cellular susceptibility to oligopeptide antifungals by stimulating their uptake via oligopeptide permeases.

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