Nucleic acid delivery using magnetic nanoparticles: the Magnetofection™ technology

2011 ◽  
Vol 2 (4) ◽  
pp. 471-482 ◽  
Author(s):  
Nicolas Laurent ◽  
Cédric Sapet ◽  
Loic Le Gourrierec ◽  
Elodie Bertosio ◽  
Olivier Zelphati
Keyword(s):  
Magnetic Nanoparticles ◽  
Nucleic Acid ◽  
Nucleic Acid Delivery

Nucleic Acid Delivery and Localizing Delivery with Magnetic Nanoparticles

2007 ◽  
pp. 23-63
Author(s):  
Christian Plank ◽  
Ulrike Schillinger ◽  
Dialekti Vlaskou ◽  
Olga Mykhaylyk
Keyword(s):  
Magnetic Nanoparticles ◽  
Nucleic Acid ◽  
Nucleic Acid Delivery

Magnetofection: The Use of Magnetic Nanoparticles for Nucleic Acid Delivery

2009 ◽  
Vol 2009 (6) ◽  
pp. pdb.prot5230-pdb.prot5230 ◽  
Author(s):  
C. Plank ◽  
J. Rosenecker
Keyword(s):  
Magnetic Nanoparticles ◽  
Nucleic Acid ◽  
Nucleic Acid Delivery

scholarly journals Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic Acid

Pharmaceutics ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 854
Author(s):  
Hai Doan Do ◽  
Christine Ménager ◽  
Aude Michel ◽  
Johanne Seguin ◽  
Tawba Korichi ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Nucleic Acid ◽  
High Efficiency ◽  
High Capacity ◽  
Cationic Liposomes ◽  
Nucleic Acid Delivery ◽  
Image Guided ◽  
Liposome Preparation ◽  
Magnetic Resonance Imaging Contrast ◽  
Diagnostic Agent

Cationic liposomes have been considered as potential vectors for gene delivery thanks to their ability to transfect cells with high efficiency. Recently, the combination of diagnostic agent and therapeutic agents in the same particle to form a theranostic system has been reported. Magnetic liposomes are one of these examples. Due to the magnetic nanoparticles encapsulated in the liposomes, they can act as a drug delivery system and, at the same time, a magnetic resonance imaging contrast enhancement agent or hyperthermia. In this work, nucleic acid delivery systems based on magnetic cationic liposomes (MCLs) were developed. Two different techniques, reverse phase evaporation and cosolvent sonication, were employed for liposome preparation. Both strategies produced MCLs of less than 200 nm with highly positive charge. Enhancement of their transverse and longitudinal relaxivities r2 and r1 was obtained with both kinds of magnetic liposomes compared to free magnetic nanoparticles. Moreover, these MCLs showed high capacity to form complexes and transfect CT-26 cells using the antibiotic-free pFAR4-luc plasmid. The transfection enhancement with magnetofection was also carried out in CT26 cells. These results suggested that our MCLs could be a promising candidate for image-guided gene therapy.

Non-Viral Nucleic Acid Delivery: Key Challenges and Future Directions

2011 ◽  
Vol 8 (3) ◽  
pp. 235-244 ◽  
Author(s):  
Mahmoud Elsabahy ◽  
Adil Nazarali ◽  
Marianna Foldvari
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Delivery ◽  
Viral Nucleic Acid ◽  
Future Directions

Charge‐Conversion Strategies for Nucleic Acid Delivery

2021 ◽  
pp. 2011103
Author(s):  
Kingshuk Dutta ◽  
Ritam Das ◽  
Jewel Medeiros ◽  
Pintu Kanjilal ◽  
S. Thayumanavan
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Delivery

scholarly journals Synthetic Histidine-Rich Peptides Inhibit Candida Species and Other Fungi In Vitro: Role of Endocytosis and Treatment Implications

2006 ◽  
Vol 50 (8) ◽  
pp. 2797-2805 ◽  
Author(s):  
Jingsong Zhu ◽  
Paul W. Luther ◽  
Qixin Leng ◽  
A. James Mixson
Keyword(s):  
Nucleic Acid ◽  
Antifungal Activity ◽  
Antifungal Agents ◽  
Fungal Growth ◽  
Nucleic Acid Delivery ◽  
Histatin 5 ◽  
Ph Buffering ◽  
Endosomal Acidification

ABSTRACT A family of histidine-rich peptides, histatins, is secreted by the parotid gland in mammals and exhibits marked inhibitory activity against a number of Candida species. We were particularly interested in the mechanism by which histidine-rich peptides inhibit fungal growth, because our laboratory has synthesized a variety of such peptides for drug and nucleic acid delivery. In contrast to naturally occurring peptides that are linear, peptides made on synthesizers can be varied with respect to their degrees of branching. Using this technology, we explored whether histidine-lysine (HK) polymers of different complexities and degrees of branching affect the growth of several species of Candida. Polymers with higher degrees of branching were progressively more effective against Candida albicans, with the four-branched polymer, H2K4b, most effective. Furthermore, H2K4b accumulated efficiently in C. albicans, which may indicate its ability to transport other antifungal agents intracellularly. Although H2K4b had greater antifungal activity than histatin 5, their mechanisms were similar. Toxicity in C. albicans induced by histatin 5 or branched HK peptides was markedly reduced by 4,4′-diisothiocyanato-stilbene-2,2′-disulfonate, an inhibitor of anion channels. We also determined that bafilomycin A1, an inhibitor of endosomal acidification, significantly decreased the antifungal activity of H2K4b. This suggests that the pH-buffering and subsequent endosomal-disrupting properties of histidine-rich peptides have a role in their antifungal activity. Moreover, the ability of the histidine component of these peptides to disrupt endosomes, which allows their escape from the lysosomal pathway, may explain why these peptides are both effective antifungal agents and nucleic acid delivery carriers.

Arginine-rich hydrophobic polyethylenimine: Potent agent with simple components for nucleic acid delivery

2013 ◽  
Vol 60 ◽  
pp. 18-27 ◽  
Author(s):  
Hamideh Parhiz ◽  
Maryam Hashemi ◽  
Arash Hatefi ◽  
Wayne Thomas Shier ◽  
Sara Amel Farzad ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Delivery

Nucleic Acid Delivery with Chitosan Hydroxybenzotriazole

2010 ◽  
Vol 20 (3) ◽  
pp. 127-136 ◽  
Author(s):  
Praneet Opanasopit ◽  
Sunee Techaarpornkul ◽  
Theerasak Rojanarata ◽  
Tanasait Ngawhirunpat ◽  
Uracha Ruktanonchai
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Delivery
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