Therapeutic delivery using gene-delivery methods

2011 ◽  
Vol 2 (4) ◽  
pp. 423-426 ◽  
Author(s):  
Seppo Ylä-Herttuala ◽  
Thomas Wirth
Keyword(s):  
Gene Delivery ◽  
Delivery Methods ◽  
Therapeutic Delivery

Non-Viral Gene Delivery Methods

2013 ◽  
Vol 14 (1) ◽  
pp. 46-60 ◽  
Author(s):  
Weiwei Wang ◽  
Wenzhong Li ◽  
Nan Ma ◽  
Gustav Steinhoff
Keyword(s):  
Gene Delivery ◽  
Viral Gene ◽  
Delivery Methods ◽  
Viral Gene Delivery

scholarly journals PAMAM dendrimer roles in gene delivery methods and stem cell research

2013 ◽  
Vol 37 (5) ◽  
pp. 415-419 ◽  
Author(s):  
Nasibeh Daneshvar ◽  
Rasedee Abdullah ◽  
Fatemeh T. Shamsabadi ◽  
Chee Wun How ◽  
M. Aizat MH ◽  
...  
Keyword(s):  
Stem Cell ◽  
Gene Delivery ◽  
Stem Cell Research ◽  
Pamam Dendrimer ◽  
Delivery Methods

The inside scoop—evaluating gene delivery methods

2005 ◽  
Vol 2 (11) ◽  
pp. 875-883 ◽  
Author(s):  
Laura Bonetta
Keyword(s):  
Gene Delivery ◽  
Delivery Methods

scholarly journals Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors

2018 ◽  
Author(s):  
Koichiro Nishino ◽  
Yoshikazu Arai ◽  
Ken Takasawa ◽  
Masashi Toyoda ◽  
Mayu Yamazaki-Inoue ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Delivery ◽  
Sendai Virus ◽  
Embryonic Stem ◽  
Aberrant Methylation ◽  
Delivery Methods ◽  
Genome Wide ◽  
Human Ipscs ◽  
Episomal Vectors ◽  
Induced Pluripotent

AbstractHuman induced pluripotent stem cells (iPSCs) are established by introducing several reprogramming factors, such as OCT3/4, SOX2, KLF4, c-MYC. Because of their pluripotency and immortality, iPSCs are considered to be a powerful tool for regenerative medicine. To date, iPSCs have been established all over the world by various gene delivery methods. All methods induced high-quality iPSCs, but epigenetic analysis of abnormalities derived from differences in the gene delivery methods has not yet been performed. Here, we generated genetically matched human iPSCs from menstrual blood cells by using three kinds of vectors, i.e., retrovirus, Sendai virus, and episomal vectors, and compared genome-wide DNA methylation profiles among them. Although comparison of aberrant methylation revealed that iPSCs generated by Sendai virus vector have lowest number of aberrant methylation sites among the three vectors, the iPSCs generated by non-integrating methods did not show vector-specific aberrant methylation. However, the differences between the iPSC lines were determined to be the number of random aberrant hyper-methylated regions compared with embryonic stem cells. These random aberrant hyper-methylations might be a cause of the differences in the properties of each of the iPSC lines.

scholarly journals Genetic modification of mesenchymal stem cells to enhance their anti-tumor efficacy

2021 ◽  
pp. 1-6
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Genetic Modification ◽  
Chemokine Receptors ◽  
Viral Vectors ◽  
Viral Gene ◽  
Delivery Methods ◽  
Differentiation Potential ◽  
Viral Gene Delivery

Non-hematopoietic mesenchymal stem cells (MSCs) are widely used in regenerative medicine and tissue engineering as they possess multilineage differentiation potential and self-renewal properties. MSCs can be easily isolated from several tissues and expanded following standard cell culture procedures. MSCs have the capability of mobilization to the tumor site; so, they can automatically relocate to the tumor sites through their chemokine receptors following intravenous transplantation. In this respect, they can be used for MSC-based gene therapy. In this therapeutic technique, beneficial genes are inserted by viral and non-viral methods into MSCs that lead to transgene expression in them. Genetic modifications of MSCs have been widely studied and thoroughly investigated to further enhance their therapeutic efficacy. The current strategies of MSC-based therapies emphasize the incorporation of beneficial genes, which will enhance the therapeutic ability of MSCs and have better homing efficiency. Non-viral methods produce less toxicity and immunogenicity compared to viral gene delivery methods and thus represent a promising and efficient tool for the genetic engineering of MSCs. Several non-viral gene delivery strategies have been developed in recent decades, and some of them have been used for MSCs modification. This mini review provides an overview of current gene delivery approaches used for the genetic modification of MSCs with beneficial genes including viral and non-viral vectors.

Chitosan for gene delivery: Methods for improvement and applications

2019 ◽  
Vol 268 ◽  
pp. 25-38 ◽  
Author(s):  
Di Chuan ◽  
Tao Jin ◽  
Rangrang Fan ◽  
Liangxue Zhou ◽  
Gang Guo
Keyword(s):  
Gene Delivery ◽  
Delivery Methods
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