Chinese hamster ovary mutants for glycosylation engineering of biopharmaceuticals

Most of clinical approved protein-based drugs or under in clinical trial have a profound impact in the treatment of critical diseases. The mammalian eukaryotic cells culture approaches, particularly the CHO (Chinese Hamster Ovary) cells are mainly used in the biopharmaceutical industry for the mass-production of therapeutic protein. Recent advances in CHO cell bioprocessing to yield recombinant proteins and monoclonal antibodies have enabled the expression of quality protein. The developments of cell lines are possible to upgrade specific productivity. As a result, it holds an interesting area for academic as well as industrial researchers around the world. This review will concentrate on the recent progress of the mammalian CHO cells culture technology and the future scope of further development for the mass-production of protein therapeutics.

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Isolation and characterization of a Chinese hamster ovary mutant cell line with altered sensitivity to vaccinia virus killing.

Journal of Virology ◽

10.1128/jvi.70.7.4655-4666.1996 ◽

1996 ◽

Vol 70 (7) ◽

pp. 4655-4666 ◽

Cited By ~ 19

Author(s):

C H Bair ◽

C S Chung ◽

I A Vasilevskaya ◽

W Chang

Keyword(s):

Vaccinia Virus ◽

Cell Line ◽

Chinese Hamster Ovary ◽

Mutant Cell ◽

Chinese Hamster ◽

Mutant Cell Line ◽

Isolation And Characterization ◽

Altered Sensitivity

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Decreased phosphatidylcholine biosynthesis and abnormal distribution of CTP:phosphocholine cytidylyltransferase in cholesterol auxotrophic Chinese hamster ovary cells

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)37238-2 ◽

1997 ◽

Vol 38 (4) ◽

pp. 711-722

Author(s):

M K Storey ◽

D M Byers ◽

H W Cook ◽

N D Ridgway

Keyword(s):

Chinese Hamster Ovary ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

Ovary Cells ◽

Ctp:Phosphocholine Cytidylyltransferase ◽

Abnormal Distribution ◽

Phosphatidylcholine Biosynthesis

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Biosynthesis of lipid-linked oligosaccharides. Isolation and structure of a second lipid-linked oligosaccharide in Chinese hamster ovary cells.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)30137-0 ◽

1979 ◽

Vol 254 (8) ◽

pp. 2754-2758

Author(s):

E. Li ◽

S. Kornfeld

Keyword(s):

Chinese Hamster Ovary ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

Ovary Cells

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Regulation of phosphatidylcholine biosynthesis in mammalian cells. III. Effects of alterations in the phospholipid compositions of Chinese hamster ovary and LM cells on the activity and distribution of CTP:phosphocholine cytidylyltransferase.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)33125-9 ◽

1983 ◽

Vol 258 (2) ◽

pp. 836-839 ◽

Cited By ~ 16

Author(s):

R Sleight ◽

C Kent

Keyword(s):

Chinese Hamster Ovary ◽

Mammalian Cells ◽

Chinese Hamster ◽

Ctp:Phosphocholine Cytidylyltransferase ◽

Phosphatidylcholine Biosynthesis

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