Delivery systems to overcome the barriers of oral siRNA delivery

2016 ◽  
pp. 122-139
Author(s):  
Fabiana Testa Moura de Carvalho Vicentini ◽  
Lívia Neves Borgheti-Cardoso ◽  
Lívia Vieira Depieri ◽  
Maria Vitória Lopes Badra Bentley
Keyword(s):  
Sirna Delivery ◽  
Delivery Systems

Lipid-based siRNA Delivery Systems

2012 ◽  
Vol 39 (5) ◽  
pp. 396-401 ◽  
Author(s):  
Wen-Juan DONG ◽  
Yin-Jian ZHOU ◽  
Wei LIANG
Keyword(s):  
Sirna Delivery ◽  
Delivery Systems

scholarly journals The Nanosystems Involved in Treating Lung Cancer

Life ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 682
Author(s):  
Andreea Crintea ◽  
Alina Gabriela Dutu ◽  
Gabriel Samasca ◽  
Ioan Alexandru Florian ◽  
Iulia Lupan ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Side Effects ◽  
Chromosomal Rearrangements ◽  
Sirna Delivery ◽  
Delivery Systems ◽  
Inorganic Nanoparticles ◽  
New Methods ◽  
Different Types ◽  
Or Gene ◽  
The Many

Even though there are various types of cancer, this pathology as a whole is considered the principal cause of death worldwide. Lung cancer is known as a heterogeneous condition, and it is apparent that genome modification presents a significant role in the occurrence of this disorder. There are conventional procedures that can be utilized against diverse cancer types, such as chemotherapy or radiotherapy, but they are hampered by the numerous side effects. Owing to the many adverse events observed in these therapies, it is imperative to continuously develop new and improved strategies for managing individuals with cancer. Nanomedicine plays an important role in establishing new methods for detecting chromosomal rearrangements and mutations for targeted chemotherapeutics or the local delivery of drugs via different types of nano-particle carriers to the lungs or other organs or areas of interest. Because of the complex signaling pathways involved in developing different types of cancer, the need to discover new methods for prevention and detection is crucial in producing gene delivery materials that exhibit the desired roles. Scientists have confirmed that nanotechnology-based procedures are more effective than conventional chemotherapy or radiotherapy, with minor side effects. Several nanoparticles, nanomaterials, and nanosystems have been studied, including liposomes, dendrimers, polymers, micelles, inorganic nanoparticles, such as gold nanoparticles or carbon nanotubes, and even siRNA delivery systems. The cytotoxicity of such nanosystems is a debatable concern, and nanotechnology-based delivery systems must be improved to increase the bioavailability, biocompatibility, and safety profiles, since these nanosystems boast a remarkable potential in many biomedical applications, including anti-tumor activity or gene therapy. In this review, the nanosystems involved in treating lung cancer and its associated challenges are discussed.

Doubly dendronized poly(norbornene)s as siRNA delivery systems

Polymer ◽  
2021 ◽  
pp. 123680
Author(s):  
Sung Hyun Cho ◽  
Ho-Joong Kim ◽  
Dong-Hyun Lee ◽  
Si Kyung Yang
Keyword(s):  
Sirna Delivery ◽  
Delivery Systems

Recent advances in siRNA delivery systems for prostate cancer therapy

2021 ◽  
Vol 22 ◽  
Author(s):  
Shahin Aghamiri ◽  
Pourya Raee ◽  
Shiva Shahmohamadnejad ◽  
Sasan Shabani ◽  
Jaber Ghorbani ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Sirna Delivery ◽  
Delivery Systems ◽  
Multi Drug Resistance ◽  
New Paradigm ◽  
Polymeric Systems ◽  
Chemotherapy Drugs ◽  
Prostate Cancer Therapy ◽  
Effective Delivery ◽  
Critical Problems

: The critical problems of conventional prostate cancer therapeutic strategies like nonspecific toxicity and multi-drug resistance prompted the development and application of countless nanoparticle-based siRNA therapeutics. The main challenges to siRNA-based therapeutics becoming a new paradigm in the treatment of prostate cancer stem from the lack of safe and effective delivery systems, immune system stimulation, poor knowledge of nano-bio interactions, and limitations concerning designing, manufacturing, clinical translation, and commercialization. In this review, we provide cutting-edge advances in nanoparticle-mediated siRNA delivery carriers like polymeric systems, lipid systems, specific systems, and rigid nanoparticles for the treatment of prostate cancer. Moreover, co-delivery of conventional chemotherapy drugs with siRNA as a robust revolutionary strategy for prostate cancer combinational therapy is completely covered.

scholarly journals Natural Polysaccharides for siRNA Delivery: Nanocarriers Based on Chitosan, Hyaluronic Acid, and Their Derivatives

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2570 ◽  
Author(s):  
Inés Serrano-Sevilla ◽  
Álvaro Artiga ◽  
Scott G. Mitchell ◽  
Laura De Matteis ◽  
Jesús M. de la Fuente
Keyword(s):  
Drug Delivery ◽  
Hyaluronic Acid ◽  
Small Interfering Rna ◽  
Drug Delivery Systems ◽  
Sirna Delivery ◽  
Delivery Systems ◽  
Low Toxicity ◽  
Interfering Rna

Natural polysaccharides are frequently used in the design of drug delivery systems due to their biocompatibility, biodegradability, and low toxicity. Moreover, they are diverse in structure, size, and charge, and their chemical functional groups can be easily modified to match the needs of the final application and mode of administration. This review focuses on polysaccharidic nanocarriers based on chitosan and hyaluronic acid for small interfering RNA (siRNA) delivery, which are highly positively and negatively charged, respectively. The key properties, strengths, and drawbacks of each polysaccharide are discussed. In addition, their use as efficient nanodelivery systems for gene silencing applications is put into context using the most recent examples from the literature. The latest advances in this field illustrate effectively how chitosan and hyaluronic acid can be modified or associated with other molecules in order to overcome their limitations to produce optimized siRNA delivery systems with promising in vitro and in vivo results.

scholarly journals Switching the intracellular pathway and enhancing the therapeutic efficacy of small interfering RNA by auroliposome

2020 ◽  
Vol 6 (30) ◽  
pp. eaba5379 ◽  
Author(s):  
Md. Nazir Hossen ◽  
Lin Wang ◽  
Harisha R. Chinthalapally ◽  
Joe D. Robertson ◽  
Kar-Ming Fung ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gold Nanoparticles ◽  
Gene Silencing ◽  
Small Interfering Rna ◽  
Sirna Delivery ◽  
Delivery Systems ◽  
Ovarian Cancer Cells ◽  
Interfering Rna

Gene silencing using small-interfering RNA (siRNA) is a viable therapeutic approach; however, the lack of effective delivery systems limits its clinical translation. Herein, we doped conventional siRNA-liposomal formulations with gold nanoparticles to create “auroliposomes,” which significantly enhanced gene silencing. We targeted MICU1, a novel glycolytic switch in ovarian cancer, and delivered MICU1-siRNA using three delivery systems—commercial transfection agents, conventional liposomes, and auroliposomes. Low-dose siRNA via transfection or conventional liposomes was ineffective for MICU1 silencing; however, in auroliposomes, the same dose gave >85% gene silencing. Efficacy was evident from both in vitro growth assays of ovarian cancer cells and in vivo tumor growth in human ovarian cell line—and patient-derived xenograft models. Incorporation of gold nanoparticles shifted intracellular uptake pathways such that liposomes avoided degradation within lysosomes. Auroliposomes were nontoxic to vital organs. Therefore, auroliposomes represent a novel siRNA delivery system with superior efficacy for multiple therapeutic applications.

scholarly journals Pioneering siRNA-Mediated Protection of Mammalian Cells against Zika Virus (MR-766) Infection

Proceedings ◽  
2020 ◽  
Vol 50 (1) ◽  
pp. 45
Author(s):  
Benedita K. L. Feron ◽  
Joachim J. Bugert ◽  
Simon C. W. Richardson
Keyword(s):  
Cell Viability ◽  
Zika Virus ◽  
Mammalian Cells ◽  
Rna Binding ◽  
Sirna Delivery ◽  
Lethal Factor ◽  
Firefly Luciferase ◽  
Delivery Systems ◽  
Zikv Infection ◽  
Huh7 Cells

Here, we present empirical data documenting the siRNA-mediated protection of cells after Zika virus (ZIKV) infection. siRNAs were designed to target well-conserved sequences across the ZIKV genome. Several delivery technologies were utilized. After the electroporation of 100 nM siRNA into human hepatocyte-derived carcinoma (Huh7) cells, the Feron Zv-2 sequence (specific to the ZIKV NS3 gene) yielded a cell viability of 150.3% ± 7.4% (SEM: n = 4) (p = 0.0004) relative to the cells treated only with the virus (33.9% ± 12%, SEM: n = 4). Furthermore, 100 nM siRNA Feron Zv-4 (specific to ZIKV 3’UTR) resulted in 119.1% ± 11.2% cell viability (SEM: n = 4) relative to the control cells treated with ZIKV (p = 0.0021). The cells were electroporated with siRNA prior to ZIKV infection and viability was monitored four days after this. Additionally, two novel siRNA delivery systems were tested. The first utilized recombinant Bacillus anthracis PA83 (octomer-forming mutants), co-incubated with the N-terminal 255 amino acids of B. anthracis lethal factor (LFn) fused in-frame with the RNA binding domain for human protein kinase R (LFn-PKR) at a concentration of 50 µg/mL (each). Here, baby hamster kidney (BHK) cells, treated with 100 nM siRNA Feron Zv-1, yielded 79.0% ± 4.0% viability relative to the control (50.2% ± 1.7%, SEM: n = 3) three days after exposure to ZIKV (p = 0.0096). Finally, HeLa exosomes loaded with siRNA Feron-Zv2 were incubated with Huh7 cells prior to ZIKV infection. For the siRNA-exosome treated cells, a viability of 123% ± 46% (SEM: n = 18), relative to 8% ± 16% (SEM: n = 18) for the same concentration of control HeLa exosomes, was recorded (p = 0.0416). In each instance, 0.3 moI was used and cell viability monitored using the PierceTM Firefly Luciferase Glow Assay Kit by Thermo ScientificTM. Here, we show for the first time that siRNA can significantly reduce ZIKV-induced cell killing. Future work will require quantitating ZIKV mRNA in relation to siRNA treatment, as well as testing the siRNAs and delivery systems within more complex models.

scholarly journals Progress and perspective of inorganic nanoparticle-based siRNA delivery systems

2016 ◽  
Vol 13 (4) ◽  
pp. 547-559 ◽  
Author(s):  
Ying Jiang ◽  
Shuaidong Huo ◽  
Joseph Hardie ◽  
Xing-Jie Liang ◽  
Vincent M. Rotello
Keyword(s):  
Sirna Delivery ◽  
Delivery Systems ◽  
Inorganic Nanoparticle
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