Application of high-resolution MS in the quantification of a therapeutic monoclonal antibody in human plasma

Bioanalysis ◽  
2014 ◽  
Vol 6 (13) ◽  
pp. 1767-1779 ◽  
Author(s):  
Kevork Mekhssian ◽  
Jean-Nicholas Mess ◽  
Fabio Garofolo
Keyword(s):  
Monoclonal Antibody ◽  
High Resolution ◽  
Human Plasma ◽  
Therapeutic Monoclonal Antibody

ZB51: a monoclonal antibody reactive with human plasma cells

1993 ◽  
Vol 84 (4) ◽  
pp. 602-607
Author(s):  
R. Webster ◽  
K. Pulford ◽  
M. Jones ◽  
K. Micklem ◽  
M. Waele ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Plasma ◽  
Plasma Cells

scholarly journals A 2.8-Angstrom-Resolution Cryo-Electron Microscopy Structure of Human Parechovirus 3 in Complex with Fab from a Neutralizing Antibody

2018 ◽  
Vol 93 (4) ◽  
Author(s):  
Aušra Domanska ◽  
Justin W. Flatt ◽  
Joonas J. J. Jukonen ◽  
James A. Geraets ◽  
Sarah J. Butcher
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
High Resolution ◽  
Cultured Cells ◽  
Human Monoclonal Antibody ◽  
Neutralizing Antibody ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Human Parechovirus ◽  
Cryo Electron Microscopy

ABSTRACTHuman parechovirus 3 (HPeV3) infection is associated with sepsis characterized by significant immune activation and subsequent tissue damage in neonates. Strategies to limit infection have been unsuccessful due to inadequate molecular diagnostic tools for early detection and the lack of a vaccine or specific antiviral therapy. Toward the latter, we present a 2.8-Å-resolution structure of HPeV3 in complex with fragments from a neutralizing human monoclonal antibody, AT12-015, using cryo-electron microscopy (cryo-EM) and image reconstruction. Modeling revealed that the epitope extends across neighboring asymmetric units with contributions from capsid proteins VP0, VP1, and VP3. Antibody decoration was found to block binding of HPeV3 to cultured cells. Additionally, at high resolution, it was possible to model a stretch of RNA inside the virion and, from this, identify the key features that drive and stabilize protein-RNA association during assembly.IMPORTANCEHuman parechovirus 3 (HPeV3) is receiving increasing attention as a prevalent cause of sepsis-like symptoms in neonates, for which, despite the severity of disease, there are no effective treatments available. Structural and molecular insights into virus neutralization are urgently needed, especially as clinical cases are on the rise. Toward this goal, we present the first structure of HPeV3 in complex with fragments from a neutralizing monoclonal antibody. At high resolution, it was possible to precisely define the epitope that, when targeted, prevents virions from binding to cells. Such an atomic-level description is useful for understanding host-pathogen interactions and viral pathogenesis mechanisms and for finding potential cures for infection and disease.

scholarly journals Distinct PD-L1 binding characteristics of therapeutic monoclonal antibody durvalumab

2017 ◽  
Vol 9 (1) ◽  
pp. 135-139 ◽  
Author(s):  
Shuguang Tan ◽  
Kefang Liu ◽  
Yan Chai ◽  
Catherine W.-H. Zhang ◽  
Shan Gao ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Binding Characteristics ◽  
Therapeutic Monoclonal Antibody

Detection and identification of a serine to arginine sequence variant in a therapeutic monoclonal antibody

2011 ◽  
Vol 879 (27) ◽  
pp. 2877-2884 ◽  
Author(s):  
Diya Ren ◽  
Jian Zhang ◽  
Ross Pritchett ◽  
Hongbin Liu ◽  
Jennifer Kyauk ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Sequence Variant ◽  
Therapeutic Monoclonal Antibody ◽  
Detection And Identification

An improved capillary isoelectric focusing-mass spectrometry method for high-resolution characterization of monoclonal antibody charge variants

2022 ◽  
Author(s):  
Tian Xu ◽  
Linjie Han ◽  
Alayna M. George Thompson ◽  
Liangliang Sun
Keyword(s):  
Mass Spectrometry ◽  
Monoclonal Antibody ◽  
High Resolution ◽  
Isoelectric Focusing ◽  
Capillary Isoelectric Focusing ◽  
Spectrometry Method ◽  
Mass Spectrometry Method ◽  
Charge Variants

Routine and high-resolution characterization of monoclonal antibody (mAb) charge variants is vital for controlling mAb quality as therapeutics. Capillary isoelectric focusing-mass spectrometry (cIEF-MS) has emerged as a powerful tool for...

Sculpting therapeutic monoclonal antibody N-glycans using endoglycosidases

2022 ◽  
Vol 72 ◽  
pp. 248-259
Author(s):  
Beatriz Trastoy ◽  
Jonathan J. Du ◽  
Mikel García-Alija ◽  
Chao Li ◽  
Erik H. Klontz ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Therapeutic Monoclonal Antibody
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