Free versus total ligand-binding assays: points to consider in biotherapeutic drug development

Bioanalysis ◽  
2011 ◽  
Vol 3 (11) ◽  
pp. 1163-1165 ◽  
Author(s):  
Jihong Yang ◽  
Valerie Quarmby
Keyword(s):  
Drug Development ◽  
Ligand Binding ◽  
Binding Assays

Critical reagents in flow cytometry, instrumentation and application in drug discovery development

Bioanalysis ◽  
2021 ◽  
Author(s):  
Vellalore N Kakkanaiah ◽  
Katie Matys ◽  
Patrick Bennett
Keyword(s):  
Flow Cytometry ◽  
Drug Discovery ◽  
Drug Development ◽  
Ligand Binding ◽  
Specific Interaction ◽  
Flow Cytometer ◽  
Analysis Tool ◽  
Binding Assays ◽  
Cellular Analysis ◽  
Main Components

Flow cytometer is a powerful cellular analysis tool consists of three main components; fluidics, optics and electronics. Flow cytometry methods have been used in all stages of drug development as like ligand binding assays (LBA). Both LBA and flow cytometry methods require specific interaction between the critical reagents and the analytes. Antibodies and their conjugates, viable dyes and permeabilizing buffer are the main critical reagents in flow cytometry methods. Similarly, antibodies, engineered proteins and their conjugates are the main critical reagents in LBA. The main difference between the two methods is the lack of true reference standards for flow cytometry cellular analysis.

Laboratory automation of high-quality and efficient ligand-binding assays for biotherapeutic drug development

Bioanalysis ◽  
2013 ◽  
Vol 5 (13) ◽  
pp. 1635-1648 ◽  
Author(s):  
Jin Wang ◽  
Vimal Patel ◽  
Daniel Burns ◽  
John Laycock ◽  
Kinnari Pandya ◽  
...  
Keyword(s):  
Drug Development ◽  
Ligand Binding ◽  
Laboratory Automation ◽  
Binding Assays ◽  
High Quality

Ligand Binding Assays in Drug Development

2013 ◽  
pp. 647-657
Author(s):  
Jeffrey M. Sailstad ◽  
Ronald R. Bowsher ◽  
Omar F. Laterza ◽  
William Nowatzke
Keyword(s):  
Drug Development ◽  
Ligand Binding ◽  
Binding Assays

scholarly journals Recommendations for Use and Fit-for-Purpose Validation of Biomarker Multiplex Ligand Binding Assays in Drug Development

2015 ◽  
Vol 18 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Darshana Jani ◽  
John Allinson ◽  
Flora Berisha ◽  
Kyra J. Cowan ◽  
Viswanath Devanarayan ◽  
...  
Keyword(s):  
Drug Development ◽  
Ligand Binding ◽  
Binding Assays ◽  
Fit For Purpose

Development of Ligand-Binding Assays for Drug Development Support

2009 ◽  
pp. 39-79 ◽  
Author(s):  
Masood N. Khan ◽  
Proveen D. Dass ◽  
John H. Leete ◽  
Richard F. Schuman ◽  
Michele Gunsior ◽  
...  
Keyword(s):  
Drug Development ◽  
Ligand Binding ◽  
Binding Assays ◽  
Development Support

Application of AlphaLISA in bioanalysis

2020 ◽  
pp. 16-37
Author(s):  
Stanislav Cherepushkin
Keyword(s):  
Drug Development ◽  
Ligand Binding ◽  
High Throughput ◽  
Protein Interactions ◽  
High Throughput Screening ◽  
High Sensitivity ◽  
Medical Diagnostics ◽  
Protein Protein Interactions ◽  
Binding Assays ◽  
Post Translational Modifications

The use and development of biotherapeutics increases and the need for accurate, sensitive and robust bioanalytical methods is also increasing. ELISA and other ligand-binding assays are the most widely used methods for the quantification of macromolecules in complex biological samples. One of the alternatives to ELISA is AlphaLISA — a versatile chemiluminescent ligand binding assay using a homogeneous no-wash protocol. AlphaLISA assays are suited for automation and exhibit high sensitivity, high throughput and wide analytical range. Since the early 2000s, this method has been used in science, medicine, and drug development for wide variety of applications, including the quantification of analytes, immunogenicity, protein-protein interactions, enzyme activity, post-translational modifications and epigenetics. In this review, we describe the principles of the AlphaLISA assay and its application in bioanalytical studies (pharmacokinetics and immunogenicity) and high-throughput screening in drug development, medical diagnostics and pathogens detection.

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