Using a microorganism consortium for consolidated bioprocessing cellulosic ethanol production

Biofuels ◽  
2011 ◽  
Vol 2 (5) ◽  
pp. 569-575 ◽  
Author(s):  
Ran Du ◽  
Shizhong Li ◽  
Xiaoqing Zhang ◽  
Chenyu Fan ◽  
Li Wang
Keyword(s):  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Consolidated Bioprocessing

Enhanced cellulosic ethanol production via consolidated bioprocessing by Clostridium thermocellum ATCC 31924☆

2018 ◽  
Vol 250 ◽  
pp. 860-867 ◽  
Author(s):  
Nisha Singh ◽  
Anshu S. Mathur ◽  
Ravi P. Gupta ◽  
Colin J. Barrow ◽  
Deepak Tuli ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Clostridium Thermocellum ◽  
Consolidated Bioprocessing

scholarly journals Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production

2020 ◽  
Author(s):  
Ce Dong ◽  
Jie Qiao ◽  
Xinping Wang ◽  
Wenli Sun ◽  
Lixia Chen ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Ethanol Production ◽  
Carboxymethyl Cellulose ◽  
Cellulosic Ethanol ◽  
Consolidated Bioprocessing ◽  
Surface Display ◽  
Cell Factory ◽  
Carbohydrate Binding ◽  
Surface Assembly

Abstract Backgrounds: Engineering yeast as a consolidated bioprocessing (CBP) microorganism by surface assembly of cellulosomes has been aggressively utilized for cellulosic ethanol production. However, most of the previous studies focused on Saccharomyces cerevisiae, achieving efficient conversion of phosphoric acid-swollen cellulose (PASC) or microcrystalline cellulose (Avicel) but not carboxymethyl cellulose (CMC) to ethanol, with an average titer below 2 g/L. Results: Harnessing an ultra-high-affinity IM7/CL7 protein pair, here we describe a method to engineer Pichia pastoris with minicellulosomes by in vitro assembly of three recombinant cellulases including an endoglucanase (EG), an exoglucanase (CBH) and a β-glucosidase (BGL), as well as a carbohydrate binding module (CBM) on the cell surface. For the first time, the engineered yeasts enable efficient and direct conversion of CMC to bioethanol, observing an impressive ethanol titer of 5.1 g/L. Conclusions: The research promotes the application of P. pastoris as a CBP cell factory in cellulosic ethanol production and provides a promising platform for screening the cellulases from different species to construct surface-assembly celluosome.

scholarly journals Cellulosic ethanol production via consolidated bioprocessing at 75 °C by engineered Caldicellulosiruptor bescii

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Daehwan Chung ◽  
Minseok Cha ◽  
Elise N. Snyder ◽  
James G. Elkins ◽  
Adam M. Guss ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Consolidated Bioprocessing ◽  
Caldicellulosiruptor Bescii

scholarly journals Correlation of Genomic and Physiological Traits of Thermoanaerobacter Species with Biofuel Yields

2011 ◽  
Vol 77 (22) ◽  
pp. 7998-8008 ◽  
Author(s):  
Christopher L. Hemme ◽  
Matthew W. Fields ◽  
Qiang He ◽  
Ye Deng ◽  
Lu Lin ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Metabolic Flux ◽  
Consolidated Bioprocessing ◽  
Genome Structure ◽  
Whole Genome Sequence ◽  
Comparative Genomic ◽  
Microarray Gene Expression ◽  
Content Type ◽  
Ethanol Yields

ABSTRACTThermophilic anaerobic noncellulolyticThermoanaerobacterspecies are of great biotechnological importance in cellulosic ethanol production due to their ability to produce high ethanol yields by simultaneous fermentation of hexose and pentose. Understanding the genome structure of these species is critical to improving and implementing these bacteria for possible biotechnological use in consolidated bioprocessing schemes (CBP) for cellulosic ethanol production. Here we describe a comparative genome analysis of two ethanologenic bacteria,Thermoanaerobactersp. X514 andThermoanaerobacter pseudethanolicus39E. Compared to 39E, X514 has several unique key characteristics important to cellulosic biotechnology, including additional alcohol dehydrogenases and xylose transporters, modifications to pentose metabolism, and a complete vitamin B12biosynthesis pathway. Experimental results from growth, metabolic flux, and microarray gene expression analyses support genome sequencing-based predictions which help to explain the distinct differences in ethanol production between these strains. The availability of whole-genome sequence and comparative genomic analyses will aid in engineering and optimizingThermoanaerobacterstrains for viable CBP strategies.

Production and Application of Lignin-Based Chemicals and Materials in the Cellulosic Ethanol Production: An Overview on Lignin Closed-Loop Biorefinery Approaches

2021 ◽  
Author(s):  
Carlos Eduardo de Araújo Padilha ◽  
Cleitiane da Costa Nogueira ◽  
Bárbara Ribeiro Alves Alencar ◽  
Íthalo Barbosa Silva de Abreu ◽  
Emmanuel Damilano Dutra ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Closed Loop

scholarly journals Single-step ethanol production from raw cassava starch using a combination of raw starch hydrolysis and fermentation, scale-up from 5-L laboratory and 200-L pilot plant to 3000-L industrial fermenters

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Morakot Krajang ◽  
Kwanruthai Malairuang ◽  
Jatuporn Sukna ◽  
Krongchan Rattanapradit ◽  
Saethawat Chamsart
Keyword(s):  
Ethanol Production ◽  
Ethanol Concentration ◽  
Consolidated Bioprocessing ◽  
Scale Up ◽  
Cassava Starch ◽  
Single Step ◽  
Starch Hydrolysis ◽  
Yield Coefficient ◽  
Raw Starch ◽  
Raw Starch Hydrolysis

Abstract Background A single-step ethanol production is the combination of raw cassava starch hydrolysis and fermentation. For the development of raw starch consolidated bioprocessing technologies, this research was to investigate the optimum conditions and technical procedures for the production of ethanol from raw cassava starch in a single step. It successfully resulted in high yields and productivities of all the experiments from the laboratory, the pilot, through the industrial scales. Yields of ethanol concentration are comparable with those in the commercial industries that use molasses and hydrolyzed starch as the raw materials. Results Before single-step ethanol production, studies of raw cassava starch hydrolysis by a granular starch hydrolyzing enzyme, StargenTM002, were carefully conducted. It successfully converted 80.19% (w/v) of raw cassava starch to glucose at a concentration of 176.41 g/L with a productivity at 2.45 g/L/h when it was pretreated at 60 °C for 1 h with 0.10% (v/w dry starch basis) of Distillase ASP before hydrolysis. The single-step ethanol production at 34 °C in a 5-L fermenter showed that Saccharomyces cerevisiae (Fali, active dry yeast) produced the maximum ethanol concentration, pmax at 81.86 g/L (10.37% v/v) with a yield coefficient, Yp/s of 0.43 g/g, a productivity or production rate, rp at 1.14 g/L/h and an efficiency, Ef of 75.29%. Scale-up experiments of the single-step ethanol production using this method, from the 5-L fermenter to the 200-L fermenter and further to the 3000-L industrial fermenter were successfully achieved with essentially good results. The values of pmax,Yp/s, rp, and Ef of the 200-L scale were at 80.85 g/L (10.25% v/v), 0.42 g/g, 1.12 g/L/h and 74.40%, respectively, and those of the 3000-L scale were at 70.74 g/L (8.97% v/v), 0.38 g/g, 0.98 g/L/h and 67.56%, respectively. Because of using raw starch, major by-products, i.e., glycerol, lactic acid, and acetic acid of all three scales were very low, in ranges of 0.940–1.140, 0.046–0.052, 0.000–0.059 (% w/v), respectively, where are less than those values in the industries. Conclusion The single-step ethanol production using the combination of raw cassava starch hydrolysis and fermentation of three fermentation scales in this study is practicable and feasible for the scale-up of industrial production of ethanol from raw starch.

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