scholarly journals The E3 ubiquitin ligase Cbl-b inhibits tumor growth in multidrug-resistant gastric and breast cancer cells

Neoplasma ◽  
2017 ◽  
Vol 64 (06) ◽  
pp. 887-892 ◽  
Author(s):  
X. CHe ◽  
Y. Zhang ◽  
X. Qu ◽  
T. Guo ◽  
Y. Ma ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Growth ◽  
Cancer Cells ◽  
Ubiquitin Ligase ◽  
Breast Cancer Cells ◽  
E3 Ubiquitin Ligase ◽  
Multidrug Resistant

scholarly journals Targeting β-Transducin Repeat–Containing Protein E3 Ubiquitin Ligase Augments the Effects of Antitumor Drugs on Breast Cancer Cells

2005 ◽  
Vol 65 (5) ◽  
pp. 1904-1908 ◽  
Author(s):  
Weigang Tang ◽  
Ying Li ◽  
Duonan Yu ◽  
Andrei Thomas-Tikhonenko ◽  
Vladimir S. Spiegelman ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Ubiquitin Ligase ◽  
Breast Cancer Cells ◽  
E3 Ubiquitin Ligase ◽  
Antitumor Drugs

scholarly journals JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Yanlin Ren ◽  
Dongyin Chen ◽  
Zurong Zhai ◽  
Junjie Chen ◽  
Aiping Li ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Ubiquitin Ligase ◽  
Breast Cancer Cells ◽  
E3 Ubiquitin Ligase ◽  
Her2 Positive ◽  
Her2 Positive Breast Cancer ◽  
Positive Breast Cancer

AbstractThe overexpression of HER2 is associated with a malignant proliferation of breast cancer. In this study, we developed a non-cytotoxic JWA gene activating compound 1 (JAC1) to inhibit the proliferation of HER2-positive breast cancer cells in vitro and in vivo experimental models. JAC1 increased the ubiquitination of HER2 at the K716 site through the E3 ubiquitin ligase SMURF1 which was due to the decreased expression of NEDD4, the E3 ubiquitin ligase of SMURF1. In conclusion, JAC1 suppresses the proliferation of HER2-positive breast cancer cells through the JWA triggered HER2 ubiquitination signaling. JAC1 may serve as a potential therapeutic agent for HER2-positive breast cancer.

scholarly journals Smurf2 E3 ubiquitin ligase modulates proliferation and invasiveness of breast cancer cells in a CNKSR2 dependent manner

Cell Division ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. 2 ◽  
Author(s):  
Diana David ◽  
Sankar Jagadeeshan ◽  
Ramkumar Hariharan ◽  
Asha Nair ◽  
Radhakrishna Pillai
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Ubiquitin Ligase ◽  
Breast Cancer Cells ◽  
E3 Ubiquitin Ligase ◽  
Dependent Manner

scholarly journals The Agonist of JWA Gene JAC1 Suppresses Proliferation of Breast Cancer Through the JWA/p38/SMURF1/HER2 Signaling

2020 ◽  
Author(s):  
Yanlin Ren ◽  
Dongyin Chen ◽  
Junjie Chen ◽  
Zurong Zhai ◽  
Aiping Li ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Ubiquitin Ligase ◽  
Breast Cancer Cells ◽  
E3 Ubiquitin Ligase ◽  
Her2 Positive ◽  
Her2 Positive Breast Cancer ◽  
Her2 Signaling ◽  
Positive Breast Cancer

Abstract Background The overexpression of HER2 is associated with malignant proliferation and invasiveness in breast cancer. Although HER2-targeting drugs have been clinically applied for cancer treatment, none of them could reduce overexpressed HER2. In this study, we reported that JAC1 could suppress proliferation of breast cancer cells via degrading HER2. Methods JWA-HER2 association was analyzed by IHC in 90 paired cases of breast cancer and adjacent non-cancerous tissues. Regulatory effect of JAC1, the agonist of JWA gene, on HER2-positive breast cancer cells was studied using colony formation assay. The effect of JAC1 on the localization of HER2 was detected by immunofluorescence microscopy assay. Western blotting, RT-PCR and immunoprecipitation assay were utilized to investigate the mechanisms of JWA on regulating HER2. Finally, xenograft mouse models were established in nude mice using BT474 cells to confirm the effect of JAC1 in vivo. Results JAC1, a small molecule agonist of JWA gene, dose-dependently suppressed proliferation in HER2-positive breast cancer in vitro and in vivo through degrading HER2. The mechanistic evidences showed that JAC1 increased the ubiquitination of HER2 at the K716 through the E3 ubiquitin ligase SMURF1. Furthermore, SMURF1 was activated due to reduced expression of NEDD4, an E3 ubiquitin ligase for SMURF1 through the JWA-p38-GATA-1-NEDD4 axis. Conclusions JAC1 suppresses the proliferation in HER2-positive breast cancer through the JWA/p38/GATA-1/NEDD4/SMURF1/HER2 signaling. JAC1 may serve as a novel therapeutic agent to breast cancer.

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