scholarly journals Effect of ionizing radiation on acinar morphogenesis of human prostatic epithelial cells under three-dimensional culture conditions

Neoplasma ◽  
2012 ◽  
Vol 59 (03) ◽  
pp. 269-281 ◽  
Author(s):  
T. WANG ◽  
S. MA X. ◽  
D. KONG ◽  
H. YI ◽  
X. WANG ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Ionizing Radiation ◽  
Three Dimensional ◽  
Culture Conditions ◽  
Three Dimensional Culture ◽  
Acinar Morphogenesis

Comparison of gene expression and activation of transcription factors in organoid-derived monolayer intestinal epithelial cells and organoids

2021 ◽  
Author(s):  
Yu Takahashi ◽  
Yu Inoue ◽  
Keitaro Kuze ◽  
Shintaro Sato ◽  
Makoto Shimizu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Regulation ◽  
Intestinal Epithelial Cells ◽  
Three Dimensional ◽  
Culture Conditions ◽  
Dependent Manner ◽  
Intestinal Epithelial

Abstract Intestinal organoids better represent in vivo intestinal properties than conventionally used established cell lines in vitro. However, they are maintained in three-dimensional culture conditions that may be accompanied by handling complexities. We characterized the properties of human organoid-derived two-dimensionally cultured intestinal epithelial cells (IECs) compared with those of their parental organoids. We found that the expression of several intestinal markers and functional genes were indistinguishable between monolayer IECs and organoids. We further confirmed that their specific ligands equally activate intestinal ligand-activated transcriptional regulators in a dose-dependent manner. The results suggest that culture conditions do not significantly influence the fundamental properties of monolayer IECs originating from organoids, at least from the perspective of gene expression regulation. This will enable their use as novel biological tools to investigate the physiological functions of the human intestine.

scholarly journals Integrin α6β4E variant is associated with actin and CD9 structures and modifies the biophysical properties of cell–cell and cell–extracellular matrix interactions

2019 ◽  
Vol 30 (7) ◽  
pp. 838-850 ◽  
Author(s):  
Mengdie Wang ◽  
James P. Hinton ◽  
Jaime M. C. Gard ◽  
Joe G. N. Garcia ◽  
Beatrice S. Knudsen ◽  
...  
Keyword(s):  
Amino Acids ◽  
Extracellular Matrix ◽  
Epithelial Cells ◽  
Three Dimensional ◽  
Cytoplasmic Domain ◽  
Culture Conditions ◽  
Basal Cells ◽  
Biophysical Properties ◽  
Integrin Α6β4 ◽  
Cell Cell

Integrin α6β4 is an essential, dynamic adhesion receptor for laminin 332 found on epithelial cells, required for formation of strong cell–extracellular matrix (ECM) adhesion and induced migration, and coordinated by regions of the β4C cytoplasmic domain. β4E, a unique splice variant of β4 expressed in normal tissue, contains a cytoplasmic domain of 231 amino acids with a unique sequence of 114 amino acids instead of β4C’s canonical 1089 amino acids. We determined the distribution of α6β4E within normal human glandular epithelium and its regulation and effect on cellular biophysical properties. Canonical α6β4C expressed in all basal cells, as expected, while α6β4E expressed within a subset of luminal cells. α6β4E expression was induced by three-dimensional culture conditions, activated Src, was reversible, and was stabilized by bortezomib, a proteasome inhibitor. α6β4C expressed in all cells during induced migration, whereas α6β4E was restricted to a subset of cells with increased kinetics of cell–cell and cell–ECM resistance properties. Interestingly, α6β4E presented in “ringlike” patterns measuring ∼1.75 × 0.72 microns and containing actin and CD9 at cell–ECM locations. In contrast, α6β4C expressed only within hemidesmosome-like structures containing BP180. Integrin α6β4E is an inducible adhesion isoform in normal epithelial cells that can alter biophysical properties of cell–cell and cell–ECM interactions.

KGF, rather than HGF, is a paracrine growth factor in the three-dimensional culture conditions

1998 ◽  
Vol 16 ◽  
pp. S84
Author(s):  
Makoto Tsunenaga ◽  
Yukiko Kurihara ◽  
Satoshi Amano ◽  
Toshio Nishiyama ◽  
Yasuhisa Nakayama ◽  
...  
Keyword(s):  
Growth Factor ◽  
Three Dimensional ◽  
Culture Conditions ◽  
Three Dimensional Culture ◽  
Paracrine Growth Factor

Macrophage involvement affects matrix stiffness-related influences on cell osteogenesis under three-dimensional culture conditions

2018 ◽  
Vol 71 ◽  
pp. 132-147 ◽  
Author(s):  
Xiao-Tao He ◽  
Rui-Xin Wu ◽  
Xin-Yue Xu ◽  
Jia Wang ◽  
Yuan Yin ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Culture Conditions ◽  
Matrix Stiffness ◽  
Three Dimensional Culture
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